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H Fields

Publications and source records attributed to H Fields.

43 records · Page 3Linked to original sources

Nurse education in the market place.

The education and training of health service professionals and non-professionals is set to be driven by a market-place philosophy which will exert and endorse competition and control via rapidly evolving change and reform. Helen Field's article seeks to provide an outline of such reform within the National Health Service education and training arena. In particular, it considers how the education of nurse professionals will fare in a system which focuses more accurately on efficiency, cost effectiveness, quality and marketing of educational provision.

Economic Competition↗

[Characterization of a panel of monoclonal antibodies to hepatitis C NS3 recombinant protein ].

Recombinant protein rNS3 imitating helicase region (1356-1459 amino acid residues) of hepatitis C virus (HCV) was expressed in E. coli cells and used for BALB/c mice immunization. Seven hybrydoma clones producing monoclonal antibodies (MAbs) to rHS3 were obtained. All MAbs reacted in ELISA with NS3 protein from Murex anti-HCV Version III and in immunoblotting from RIBA 3. These MAbs detect 5 individual epitopes, 4 of which were conformational and 1 discontinuous. All MAbs could compete for rNS3 binding with serum antibodies from patients with chronic hepatitis C, which suggests that these MAbs can recognize the natural HCV NS3 protein.

Animals↗

Orthodontic diagnosis and treatment planning in the primary dentition.

Early recognition of malocclusions is in the hands of the primary care providers. Pediatric and general dentists should be familiar with conditions that are known to interfere with growth and development of a child. Conditions such as early loss of primary teeth, anterior and posterior crossbites, and some Class III skeletal problems can be treated in the primary dentition to facilitate normal growth and development and offered some benefit to the patients. On the other hand, aggressive arch length modulation, Class II correction and intervention for vertical problems, including non-nutritive sucking habits, is largely unwarranted.

Child, Preschool↗

[Immunochemical properties and specificity of monoclonal antibodies against N- and C-terminal sites of the recombinant protein of the nucleocapsid of hepatitis C virus (HCcAg)].

Highly affine murine monoclonal antibodies (MAB) to recombinant nucleocapsid (core) protein of hepatitis C virus (rHCcAg) expressed in E. coli were obtained. The MABs were analyzed by solid-phase enzyme immunoassay (EIA), immunodot, immunoblotting, and competitive immunochemical analysis. For estimating the epitope specificity of MAB, several immunoreactive fragments of different length were cloned from the HCcAg region overlapping 160 N-terminal amino acid (a. a.) residues. Use of these fragments and the competitive EIA demonstrated that MAB recognize 4 non-overlapping epitopes, 2 of which are localized in the 1-80 a. a. and 2 other in the 80-150 a. a. regions. A protocol of EIA for detecting HCcAg using MABs to two nonoverlapping HCcAg epitopes has been designed. The sensitivity of double-site sandwich is 1 ng/ml for the recombinant protein.

Animals↗