Biomedical subjects

H Fisch

Publications and source records attributed to H Fisch.

39 records · Page 3Linked to original sources

PubMedDate not supplied

Testicular sperm aspiration (TESA) and its application in oocyte donation.

This study was conducted to assess the outcomes of TESA in women undergoing oocyte donation. Descriptive reports from a university IVF practice were reviewed to evaluate the outcomes of women (n = 10) who underwent 12 cycles of ovum donation and required testicular sperm aspiration due to obstructive azoospermia (n = 6) and nonobstructive azoospermia (n = 4). Percutaneous needle aspiration of the testes was performed prior to oocyte retrieval to recover sperm, and intracytoplasmic sperm injection was subsequently performed on the donated oocytes. Fertilization was documented 16-18 h later and transcervical embryo transfer performed 72 h postretrieval. Successful retrieval of testicular spermatozoa utilizing the percutaneous technique was achieved in all 12 cycles. All cycles resulted in an ET with the achievement of 7 (58%) pregnancies, of which 5 (42%) are ongoing or delivered. The individual embryo implantation rate was 18.4% (9/49). Two cycles (16.6%) produced supernumerary embryos for cryopreservation. TESA represents a simple procurement technique associated with minimal morbidity. It may be offered even in cases of nondestructive azoospermia.

Adult↗

PubMedDate not supplied

Modulation of porcine peripheral blood-derived macrophage functions by in vitro infection with African swine fever virus (ASFV) isolates of different virulence.

Macrophages play an important role in defense against invading pathogens and neoplastic diseases. Their ability to maintain their functional characteristics is also important in the survival of the host. Loss of macrophage function during infection may be important in determining resistance or susceptibility of a host species. In this communication, we describe the effect of ASFV replication, in macrophages in vitro, on the functional characteristics of these cells. Infection with ASFV did not alter the expression of Fc receptors nor their ability to mediate ADCC. However, African swine fever virus was shown to modulate antibody mediated phagocytosis, chemiluminescence and chemotaxis. Similar modulation of certain macrophage functions was observed with ASFV isolates that differed in their virulence. We were therefore, unable to correlate the virulence of ASFV with the ability to alter macrophage function.

African Swine Fever↗

PubMedDate not supplied

Variation in antisperm antibody response following transection of male genital tract in Lewis rats.

Genital tracts of male Lewis rats were transected at various levels to determine whether this may influence antisperm antibody response. Adult male rats underwent bilateral transection of the vas deferens (group I, n = 9), mid-epididymis (group II, n = 10), and efferent duct (group III, n = 9). Group IV (n = 10) underwent a sham operation. Sera were collected by retro-orbital puncture before the operative procedure and monthly for 3 months postprocedure. Sperm-reactive immunoglobulins IgG, IgA, and IgM were measured individually as well as combined in serum by enzyme-linked immunosorbent assay (ELISA) using lithium diiodosalicylate (LIS)-solubilized washed rat caudal epididymal sperm. The maximal immune response was seen in all groups at 2 months postprocedure. Antibody response defined as the net ELISA absorbance reading for the combined immunoglobulin group were (mean +/- SEM): group I = 120 +/- 16, group II = 156 +/- 23, group III = 190 +/- 20, and group IV = 116 +/- 22. The highest antibody response was noted in the efferent duct group, which was statistically (p < .05) greater than the sham-operated and vas deferens groups. In the efferent duct group the highest immunoglobulin response was observed in the IgG class, which was significantly higher (p < .05) than the IgA and IgM classes. The transection of the male genital tract at different levels leads to variation in antisperm antibody response and that sperm located at different sites along the genital tract may differ in their autoantigenic potential.

Animals↗