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Biomedical subjects

H Fleury

Publications and source records attributed to H Fleury.

At least 19 recordsLinked to original sources
PubMed1992

Comparative assessment of quantitative HIV viraemia assays.

OBJECTIVE: To compare two published methods for determining plasma viraemia in HIV-seropositive patients, with reference to a cellular viraemia assay. PATIENTS, PARTICIPANTS: Three patient groups were defined according to CD4 cell count: group I, less than 200 x 10(6)/l (23 patients); group II, 200-500 x 10(6)/l (18 patients); and group III, greater than 500 x 10(6)/l (13 patients). METHODS: The two reported methodologies were applied to all fresh samples, simultaneously and on the same day. RESULTS: The two techniques did not differ significantly in the detection of plasma viraemia: 82.3% of group I patients and 55% of group II patients were positive, while all group III patients were negative. Cellular viraemia was positive for 96% of the overall population. CONCLUSIONS: These results, obtained in a network of seven French laboratories involved in clinical trials, confirm that both plasma viraemia and cellular viraemia are useful virological markers.

AIDS Serodiagnosis↗
PubMed1991

[Virologic diagnosis of meningoencephalitis].

Many viruses can be responsible for meningoencephalitis. The principal ones are listed and the main features of their virological diagnosis are described. It must be borne in mind that a virological diagnosis must always be guided by the clinical context and that viral meningoencephalitis, relatively uncommon in Europe, is much more frequent in tropical countries.

Acute Disease↗
PubMed1988

Isolation of HIV in a seronegative demented patient without symptoms of immune deficiency.

A 60-year-old male patient, originating from West Africa, developed acute and regressive neurologic symptoms associated with aphasia, apraxia, acalculia, behavioral impairments, and an epileptic phase. Eighteen months after the onset of the disease, the patient was almost normal. All along the clinical course, biological abnormality patterns were minor. We noted only a mild neutropenia in the blood. We also observed a weak lymphocytosis and elevated protein content in the cerebrospinal fluid. Electroencephalogram examination revealed slow waves which disappeared after remission. A weak ventricular dilatation was detected on CT scan. Neither vascular, nor tumoral, nor a classical infectious origin could be identified. While the patient was seronegative to HIV, a HIV-like virus was isolated twice from his peripheral blood lymphocytes during the disease. Eighteen months later, the patient remained seronegative. He developed neither AIDS nor immunodeficiency. The subtype of HIV has been isolated and characterized, and its neurotropism is being investigated.

Antibodies, Monoclonal↗
PubMed1987

[Particles with retrovirus appearance and reverse transcriptase activity in cell cultures derived from lymph node biopsies in Hodgkin's disease].

Long term cultures of Reed-Sternberg like cells were obtained from lymph node biopsies of two patients suffering from Hodgkin's disease. As soon as the 15th day of culture, a weak magnesium-dependent reverse transcriptase activity was observed in the cell culture supernatants. Retroviral-like particles were observed in cell cultures as well as in their supernatants.

Adult↗
PubMed1984

[Prevention of poliomyelitis: live or killed vaccine? Remarks apropos of 2 cases of poliomyelitis].

The attenuated poliovirus vaccine is remarkably effective in preventing the disease but its innocuity has always been questioned. It seems to be responsible for occasional cases of spinal paralysis occurring in developed countries where poliomyelitis has virtually been eradicated. The authors report two cases of acute anterior poliomyelitis in young adults, in which the poliovirus recovered from the faeces might have been of vaccinal origin. Virological techniques do not always characterize the strains isolated, nor distinguish between a vaccinal mutant and a wild poliovirus. These data, together with previously published cases, have prompted the authors to discuss whether the use of a live poliovaccine is justified.

Adult↗
PubMed1982

[45Ca flow from cultured pituitary cells: effect of TRH and potassium].

Potassium (KCl: 50 mM) increases the uptake of 45Ca by GH3/B6 pituitary cells whereas TRH (100 nM) does not modify the uptake. On the contrary, 45Ca efflux is quickly and transiently increased by TRH whereas KCl has no influence on the 45Ca outflow. Our results suggest that these two secretagogues act on the GH3 pituitary cells by different processes.

Animals↗
PubMed1979

Membrane effects of thyrotropin-releasing hormone and estrogen shown by intracellular recording from pituitary cells.

The effects of thyrotropin-releasing hormone and 17 beta-estradiol on the electrical membrane properties of a prolactin-secretin pituitary cell line (GH3/B6) were studied with intracellular microelectrode recordings. Of the cells tested, 50 percent were excitable and displayed calcium-dependent action potentials when depolarized. When injected directly on the membrane of an excitable cell, thyrotropin-releasing hormone and 17 beta-estradiol induced action potentials within 1 minute. The spiking activity was preceded by a progressive increase of the input resistance without any detectable change in the resting membrane polarization. The results reveal a rapid effect of both substances on the membrane of GH3/B6 cells. In the case of thyrotropin-releasing hormone, which has both a short-term effect on release of prolactin and a long-term effect on its synthesis, the induced electrical activity may be associated with the stimulation of prolactin production. The physiological implication of 17 beta-estradiol-induced, calcium-dependent spiking activity remains to be elucidated.

Action Potentials↗
PubMed1977

An in vitro study of the susceptibility of cells from a meningioma of human origin for measles virus.

A cell culture from a meningioma of human origin was found permissive for the Edmonston strain of measles virus. Virus was isolated from the supernatant of infected cell sheet as soon as 48 hr post-inoculation. Cytopathic effect, observed by light microscopy, exhibited syncitia plus intracytoplasmic and intranuclear inclusions. Electron microscopy enabled viral nucleocapsids to be observed first in the cytoplasm and secondly within the nucleus of infected cells.

Brain Neoplasms↗
PubMed1975

[Lymphoblast transformation test in osteoarticular tuberculosis].

Bacteriological proof of an osteoarticular tuberculous lesion is difficult to obtain, notably in Pott's disease. The authors, on the basis of earlier work in the field of pulmonary pathology, wished to assess the value of the lymphocyte transformation test in the diagnosis of this disease. Old tuberculin from the Pasteur Institute and LP48 tuberoulin were used at different concentrations. Out of 10 tuberculous patients (8 with Pott's disease and 2 with white tumours), 9 had positive tests, of which 7 were strongly positive ; the average transformation value was 13 percent. This positiveness was a function of the condition of the patient, of the extent of the lesions, and of the treatment. Of the 25 control patients affected by an infectious, nonmicrobial or other osteoarticular process, 17 gave negative results in the test, and 8 were positive, two of whom had high values. The average transformation value was 4 percent. From this study it seems that a negative result in the LTT is a valuable argument against the diagnosis of osteoarticular tuberculois ; a weak positive result indicates tuberculin hypersensitivity without absolutely defining evolution of tuberculois ; a strongly positive test may be encountered in hyperergic states connected with prolonged infectious conditions in bones or joints.

Bone Diseases↗
PubMedDate not supplied

Dopamine inhibition of action potentials in a prolactin secreting cell line is modulated by oestrogen.

Secretory activity of the anterior pituitary gland is regulated by the brain through stimulatory and inhibitory substances released from nerve endings in the median eminence of the hypothalamus and carried by the adenohypophysial portal blood system to their respective target cells. These hypothalamic influences are modulated by the feedback action of peripheral hormones. Prolactin (PRL) secreting cells are, at least partially, under the stimulatory influence of thyrotropin releasing hromone (TRH) and of oestrogens. However, they are mainly controlled by inhibitory substances among which dopamine (DA) is one of the most potent in vivo as well as in vitro. The inhibitory effect of DA is reversed by oestrogen in vitro. The mechanism by which these factors interact on their target cells is poorly understood. The recent discovery that anterior pituitary cells are excitable and that they are able to generate Ca2+-dependent action potentials has led to the suggestion that these effects are involved in a stimulus-secretion coupling at the membrane level. In this paper, we report that DA inhibits both the spontaneous and TRH-induced action potentials in clonal PRL pituitary cells. In addition, oestradiol-17 beta is able to reverse the inhibitory effect of DA.

Action Potentials↗