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H Fritsche

Publications and source records attributed to H Fritsche.

32 records · Page 2Linked to original sources
PubMed1990

Immunohistochemical analysis of P-glycoprotein expression correlated with chemotherapy resistance in locally advanced breast cancer.

We analyzed the expression of P-glycoprotein in samples from 48 patients with locally advanced breast cancer. Tumor samples from 40 patients were obtained at mastectomy, which was performed after three cycles of induction chemotherapy consisting of doxorubicin, cyclophosphamide, vincristine, and prednisone. P-glycoprotein expression distributed focally was observed in 20 tumors by the immunoperoxidase method using the anti-p170-monoclonal antibody C219. The percentage of the tumor cell population expressing P-glycoprotein varied from less than 5% to greater than 30%; expression was observed significantly more often in tumors that showed less than partial response to the preoperative chemotherapy. Furthermore, P-glycoprotein was not expressed in eight tumor specimens obtained at the time of diagnosis, prior to chemotherapy, from patients who subsequently had pathologic complete responses. A comparative study of P-glycoprotein expression before and after chemotherapy and upon recurrence of tumor was done on a limited number of samples. No significant differences in P-glycoprotein expression were found. Therefore, it is possible that an intrinsic, rather than acquired, drug resistance may play a role in the failure of induction chemotherapy for locally advanced breast cancer.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1990

Squamous cell carcinoma antigen (TA-4) in penile carcinoma.

TA-4 antigen, originally isolated from women with squamous cell carcinoma of the cervix, is elevated in the sera of patients with squamous cell carcinomas of several sites, including esophagus, lungs, and head and neck. In this study, we compared the serum levels of TA-4 in normal volunteers, patients with resected penile squamous cell carcinoma, and patients with metastatic penile squamous cell carcinoma. TA-4 values were elevated in 5 of 11 patients (45%) who had metastatic disease. In 2, TA-4 was normal the first time metastasis was clinically detected but rose as the disease progressed. Moreover, in 3 patients in whom serial determinations were made, serum TA-4 values correlated well with disease progression and response to treatment. We conclude that TA-4 values are elevated in some patients with metastatic squamous cell carcinoma of the penis and may become a useful marker for monitoring response to therapy.

Antigens, Neoplasm↗
PubMed1989

c-erbB-2 amplification in node-negative human breast cancer.

c-erbB-2 gene analysis by Southern and DNA dot blot methods was done in 66 tumor samples from patients with histologically node-negative breast cancer. The c-erbB-2 gene was amplified 2- to greater than 8-fold in 13 tumors (20%). None of 59 tumors that were examined by the Southern method showed c-erbB-2 gene rearrangement. c-erbB-2 amplification was analyzed in relation to other prognostic factors. The c-erbB-2 gene was amplified in five of 36 (14%) diploid and eight of 30 (27%) aneuploid tumors. Thirteen of 54 (24%) tumors with nuclear Grade 1 or 2 displayed c-erbB-2 amplification, whereas none of 12 tumors with nuclear Grade 3 did. No correlation was observed with estrogen receptor content, tumor size, histological type, or age of patients. The median follow-up date for these patients was 85+ mo. Of 13 patients whose tumors showed c-erbB-2 amplification, six patients (46%) developed recurrence, and five patients (38%) died of metastatic disease. In contrast, of 53 patients whose tumors did not show c-erbB-2 amplification, 15 patients (28%) developed recurrence, and seven patients (13%) died of disease. In conclusion, our results show that c-erbB-2 gene amplification was more frequent in aneuploid tumors and tumors with poor nuclear grade. c-erbB-2 amplification may be considered a possible prognostic factor in node-negative breast cancer.

Adult↗
PubMed1989

[Automated microfluorometric absolute count and maturation analysis of reticulocytes].

The absolute reticulocyte count and the maturation distribution of blood reticulocytes can be obtained by automated microfluorometric analysis in a deposit system called AURAS (Automated Reticulocyte Analysis System). Moreover visual measurements are possible on the same specimen for cell individual control. Visual reticulocyte counts are of high reproducibility and accuracy and therefore deliver practicable reference data. Results from experimental work indicate the value of maturation distribution changes with respect to Erythropoietin effects.

Erythrocyte Count↗
PubMed1988

An enzyme immunoassay for carcinoembryonic antigen which employs simultaneous incubation of specimen with solid phase and enzyme-conjugated antibodies.

The Abbott CEA-EIA Monoclonal One-Step procedure was evaluated and compared to the Abbott CEA-EIA Monoclonal, a two-step assay. The reproducibility, sensitivity, recovery and dilution linearity were similar for both assays. Excellent correlations were found between the carcinoembryonic antigen (CEA) values obtained with these assays for healthy donors (n = 261, r = 0.951), patients with benign diseases (n = 171, r = 0.994) and cancer patients (n = 585, r = 0.997). In serial monitoring studies, one-step CEA values paralleled the CEA values determined with the two-step assay regardless of the type of cancer. The advantages of the one-step CEA assay over the two-step assay include significant reduction in both attended and total assay times, reduced sample volume, dry antibody-coated beads, color-coded reagents and an extended range for the standard curve. These test improvements were achieved without affecting the reproducibility, sensitivity and accuracy of the CEA measurement.

Antibodies, Monoclonal↗
PubMed1986

[Automation of hematologic diagnosis: developmental status of instruments for counting and differentiating blood cells].

There are numerous instruments for routine hematology. Most of them are flow systems and work according to the conductometric principle (Coulter), some according to scatter light or absorption principle. They provide a different number of hematologic parameters automatically or semiautomatically. For research purpose, i.e. cell or particle analysis, are different systems available, operating with scatter light or fluorescence activation; some instruments are able to sort cells in different subpopulations. Other apparatus deliver the complete differential leucocyte count by pattern recognition of conventionally stained smears.

Animals↗
PubMed1985

Beta 2 microglobulin in multiple myeloma.

Serum beta 2 microglobulin levels (B2M) were evaluated before and during chemotherapy in 97 previously untreated patients with multiple myeloma. Pretreatment values were useful in confirming tumor mass grade, and marked reductions following chemotherapy correlated well with the onset of remission. No gain was evident from correcting the B2M for the level of serum creatinine. A pretreatment B2M value greater than 6 mg/L correlated with a low response rate and was the most important variable that predicted a short survival time.

Aged↗
PubMed1982

Ploidy, proliferative activity and estrogen receptor content in human breast cancer.

Tumor samples from 80 patients with breast cancer (43 primary, 37 metastatic) were analyzed for ploidy and proliferative activity using DNA flow cytometry. Sixty-one tumors (40 primary, 21 metastatic) were also analyzed for estrogen receptor content. Eighty-five percent of all tumors had an abnormal DNA content. The majority of tumors were hyperdiploid (65%). Seventy-three tumors had a unimodal ploidy distribution, while in seven cases two distinct aneuploid subpopulations were identified. The degree of ploidy abnormality was not related to extent of disease or menopausal status, but was higher (DNA index greater than 1.5) in poorly differentiated tumors (p = 0.05). ER-positive tumors were more often diploid (7 of 31) than ER-negative tumors (3 of 30, p = 0.16). DNA content was constant in biopsies from multiple sites in 5 patients with metastatic disease and in serial biopsies over the course of the disease. High proliferative activity (percent cells in S-phase) was more often associated with ER-negative tumors than ER-positive tumors (p = 0.03). However, in all subgroups analyzed a wide range of values was noted. We conclude that flow cytometric analysis of cellular DNA content provides a rapid means of quantitating ploidy and proliferative activity in human breast cancer. Ploidy abnormalities were common, stable, and usually unimodal. Proliferative activity was inversely related to estrogen receptor content.

Aneuploidy↗
PubMed1979

[Diagnosis and therapy of idiopathic thrombocytopenic purpura].

To the diagnostics of the idiopathic thrombopenia caused by autoantibodies belong: 1. the clinical findings such as type of haemorrhage and the controls of the capillary function. 2. the proof of antibodies, in which case positive findings are proving, but negative ones do not exclude the immune thrombopenia, 3. smear of the sternal marrow with judgment of the megakaryocytes and formation of thrombocytes, 4. isotopic control of the survival time of the thrombocytes, which is reserved to special insitutions. As therapy essentially three forms have developed: 1. Therapy with glucocorticoids, 2. Immunosuppressive drugs, 3. Splenectomy. In own observations of 48 patients the glucocorticoids bring an actual improvement only for 7 patients, the others fail in therapy or show only a partial remission. The least prospects are in the immunosuppressive drugs, being equal whether preceding or accompanying a glucocorticoid therapy was performed. Most successes (11 out of 16 patients) are to be stated in splenectomy. Following an after-control of all therapeutic methods together a successful therapy results only in one third, in the next two thirds the therapy either fails or at most evokes a partial remission.

Coombs Test↗
PubMed1977

[Fluorescence microscopic demonstration of eosinophile leucocytes with different fluorochromes (author's transl)].

A screening program involving blood from men and horse resulted in about 80 fluorochromes admitting microscopic differentiation of eosinophile leucocytes (properly their granules) in blood smears. Fluorescence distinguishes either selective, i. e. only eosinophile cells show fluorescence, or specific, that means, eosinophilic granules exhibit fluorescence differing from non-eosinophilic cells by colour and/or intensity.

Animals↗
PubMed1975

[Gestation in patients with heart disease].

A brief report of the special management of pregnant women suffering from heart-disease has been given. There were 188 (0,72%) of all together 26233 deliveries in 170 women with heart-failure. Cesarean section by cardiological indications as a matter of course will be carried out only in few special cases. The management tends to vaginal delivery with shortening the last period by operating. In comparison with the period from 1946 to 1962 the mortality of the mothers decreased from 7,8% to 0,5% the mortality of the infants from 9,8% to 3,2% and the prematurity by weight from 25,5% to 8,5%.

Adult↗