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Biomedical subjects

H Gómez-Estrada

Publications and source records attributed to H Gómez-Estrada.

At least 19 recordsLinked to original sources
PubMed1983

Circulating immune complexes in hypertensive disease of pregnancy.

Circulating immune complexes in the blood of patients with hypertensive diseases of pregnancy were investigated. Sixty women with pregnancies between 30 and 40 weeks were studied. Of these patients, 22 were normal, 18 developed preeclampsia, 2 developed eclampsia, 14 had essential hypertension, and four had hypertension of renal origin. Circulating immune complexes were determined by the method of phagocytosis and immunofluorescence. They were found only in patients with preeclampsia or eclampsia, and were constituted of IgG and C3. It is suggested that the detection of circulating immune complexes by this method can be useful in the differential diagnosis of preeclampsia from other hypertensive diseases of pregnancy.

Adult↗
PubMed1981

Levamisole does not produce chromosome fractures.

Levamisole is a drug chemically related to metronidazole. Metronidazole produces chromosome alterations in animals and humans. The aim of this work was to investigate if levamisole could produce chromosome fractures in mice. Levamisole and metronidazole were injected daily during five days, subcutaneously, at pharmacological doses of 2.5 and 6.2 microgram per g of body weight respectively to BALB/c mice. Three injected and control mice were sacrificed each day and chromosome studies were performed with hematopoietic sternum cells. In control mice, forty acchrocentric chromosomes were found without fractures. Metronidazole-injected mice showed increasing percentages of chromosome fractures from the first to the fifth day of the study: 0, 1.46, 1.72, 7.70 and 9.10. Levamisole-injected mice did not show chromosome fractures at that time.

Animals↗
PubMed1980

The presence of anti-leukoblast antibodies as complement fixation agents in leukemia patients imm-nized with leukoblats treated with neuraminidase and radiation.

Six adult patients with acute myeloblastic leukemia were treated with chemotherapy and immunotherapy. This was done with cultured autologous leukoblasts, treated with neuraminidase of Vibrio cholerae and radiation, and injected six to ten times each eight to ten days between the chemotherapy cycles. Class IgC leukemic anti-leukoblast antibodies which are able to activate the C3 component of the complement system were found in the serum of patients. Five patients have been in complete remission for more than twenty months and the sixth patient for ten months. Antibodies for C3 fixation were not found in the serum of five other patients who were not immunized, nor in five healthy control subjects.

Adolescent↗
PubMed1980

Phagocytic activity of human polymorphonuclear leukocytes at different temperatures.

Phagocytic activity from human PMN obtained from defibrinated peripheral blood of young adults of both sexes was studied. Defibrinated blood was distributed in volumes of 0.6 ml over 12 glass slip covers, and incubated at 37 degrees C in humid chamber for 30 minutes, in order to permit the PMN adherence to glass. Then the PMN were rinsed with culture medium, covered with 0.6 ml of 1 x 10(6) latex particles (0.81 microns, Bacto-latex Difco) suspension in autologous serum by milliliter, and incubated by triplicate per 30 minutes at 35 degrees C, 37 degrees C and 39 degrees C. Then slip covers were rinsed with culture medium and stained with Wright stain. Phagocytic index was calculated as the average of phagocytized latex particles per 300 PMN. The results obtained were: at 35 degrees C, 17.26 +/- 6.1; at 37 degrees C, 19.80 +/- 6.26; at 39 degrees C, 21.94 +/- 6.94; and at 41 degrees C, 25.58 +/- 6.69. The correlation coefficient between this temperature increase and the phagocytic indexes was 0.41. This allow to conclude that a direct linear increment exists between the PMN phagocytic indexes and the temperature increase.

Adult↗
PubMed1980

Location of tumoral antigens in L5178Y murine lymphoma cells.

Location of neoplasia specific tumoral antigens (ANE) were investigated in L5178Y murine cells. Cells were treated by means of concanavalin A (CoA), Vibrio cholerae neuraminidase (NVC) or a combination of both (Coa-NVC) and were inactivated by 6,000 rad irradiation. With 5 x 10(6) treated cells four groups, 15 mice in each group of the BALB/c strain were immunized weekly and 10 days latter immunity to L5178Y lymphoma cells was tested by means of intraperitoneal inoculation of 5 x 10(7) intact, viable L5178Y cells. None of the mice immunized with concanavalin A treated and irradiated cells developed the inoculated lymphoma. However all mice immunized with cells treated with CoA or CoA-NVC developed a lymphoma. Electron microscopy studies of normal L5178Y cells, irradiated or treated with NVC showed the exterior cell coating or glycocalix that was absent in those treated with CoA or CoA-CoA-NVC. These results allowed us to conclude that ANE of L5178Y cells susceptible to modification by NVC as if to induce an immunologic rejection response are located in the glycocalix of these cells.

Animals↗
PubMed1979

[Postnatal phagocytic maturation (author's transl)].

Seventy two children were studied including 12 prematures, 31 newborns, 25 infants and 4 preschool children. They were clinically healthy with exception of prematurity factor. The phagocytic index (PI) of their polymorphonuclear leukocytes was investigated against latex particles suspended in autologous serum. In prematures PI levels were from 5.55 to 11.05; in newborns and infants, 11.09 to 15.00; in preschool children, 15.08 to 21.97 and in normal adults 19.21 to 22.70. PI value showed marked increase after the first neonatal month or when children reached a body weight of over 4.250 Kg.

Aging↗