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Biomedical subjects

H Güner

Publications and source records attributed to H Güner.

12 recordsLinked to original sources
PubMed1997

Detection and typing of human papillomavirus DNAs by restriction endonuclease mapping of the PCR products.

Human papillomavirus (HPV) has been implicated strongly with human cervical, anal and penile cancers. The polymerase chain reaction (PCR) was used to detect HPV in cervical specimens of 88 women working at the public whorehouse. Using consensus primers which encode the L1 region of the HPV genome, the presence of HPV DNA was demonstrated in 2 specimens. Restriction endonuclease digestion of the amplified products was carried out for accurate typing. Samples which were positive by L1 PCR were digested with Hae III, BstN I and Dde I restriction enzymes. The patterns produced by digestion were identified as HPV types 6b and 16.

Adult↗
PubMed1996

Surgical treatment alternatives in stress incontinence.

OBJECTIVES: This study was performed to evaluate the different surgical treatment alternatives for urinary stress incontinence and their clinical results. METHODS: Of the patients who visited our clinic between January 1989 and June 1994 complaining of urinary stress incontinence, 408 were found to have genuine stress incontinence (GSI) after undergoing clinical and laboratory investigations. These 408 patients were treated with different surgical techniques. Their postoperative results are discussed. RESULTS: After postoperative follow-up, the success rates of the different surgical techniques were as follows: anterior colporrhaphy (Kelly plication) 66.2%, Marshall-Marchetti-Krantz 88.7%, Burch procedure 94.1%, and sling urethropexy 97.5%. CONCLUSIONS: Patients with a clear-cut diagnosis of GSI must be surgically treated. There are several different techniques, all of which have advantages and disadvantages. If the right technique is used for the right patient then the success rate is high.

Adult↗
PubMed1996

Endometrial assessment by vaginal ultrasonography might reduce endometrial sampling in patients with postmenopausal bleeding: a prospective study.

The purpose of this study is to compare transvaginal sonographic endometrial assessment with histology obtained by endometrial curettage in postmenopausal patients and to determine a cut-off point for endometrial thickness to reduce unnecessary diagnostic curettage for postmenopausal bleeding. A total number of 289 postmenopausal women were studied prospectively; 192 patients with postmenopausal bleeding and 97 postmenopausal women without bleeding comprised the study and control groups respectively. All patients were scanned by ultrasound transvaginally, then dilatation and curettage was performed for endometrial evaluation. In all women with and without postmenopausal bleeding, endometrial thickness of 4 mm or less as depicted by sonography correlated well with 'atrophic endometrium' (100%). Of 158 women with an endometrium > 4 mm in both groups, 40 women (25.1%) had a negative result (tissue insufficient for diagnosis or atrophic endometrium), while 118 patients (74.8%) had a positive result which included proliferative and secretory endometrium, endometrial hyperplasia, polyps and cancer. As the endometrial thickness increased, the probability of finding pathology with curettage was increased linearly in postmenopausal bleeding (PMB) positive and negative groups and there was a significant linear positive correlation between PMB (+) and (-) cases (Linear regression, R = 0.91, p < 0.03). A significant difference was found between endometrium carcinoma and all other endometrial pathologies concerning the mean endometrial thickness of 22.7 +/- 10.2 mm (chi-square, p < 0.0001). Also, the mean endometrial thickness of 4.7 +/- 3.3 mm for atrophic endometrium and 6.1 +/- 3.7 mm for 'tissue insufficient for histological diagnosis' were found significantly different than all other endometrial pathologies (chi-square, p < 0.0001). Endometrial thickness of < or = 4 mm may serve as cut-off point for predicting pathology negative cases with an accuracy of 100% in postmenopausal bleeding positive and negative cases. Then as the endometrial thickness increases, the probability of finding endometrial pathology in curettage increases linearly with a positive predictive value of 74.6%.

Adult↗
PubMed1995

Adult extrarenal Wilms' tumor. A case report.

Wilms' tumor or nephroblastoma is a malignant tumor of childhood and there are less than 200 cases in adults reported in the literature. An extrarenal location is also a rare situation for these tumors. A 48-year-old patient, referred to our gynecology department because of an abdominal mass, was operated on with a preoperative diagnosis of a possible ovarian tumor. The diagnosis of an extrarenal Wilms' tumor (ERWT) was made pathologically. Since it is a very rare entity, ERWT of adult origin is presented and the related literature is reviewed.

Abdominal Neoplasms↗
PubMed1995

Morphometric analysis of gap junctions in nonpregnant and term pregnant human myometrium.

BACKGROUND AND METHODS: Some structures in the human myometrium, namely; caveolae, extracellular space and gap junctions were quantified in tissues from nonpregnant women, women who were not in labor, and women in spontaneous labor or oxytocin-induced labor, in order to reveal morphologic differences that would contribute to explaining the events leading to labor. Transmission electron micrographs taken from tissues from twenty-four pregnant and ten nonpregnant women were studied. RESULTS: Differences were observed in cell growth which was accompanied by enlargement of the extracellular space, and in gap junctions. Gap junctions were demonstrated in nonpregnant women, and even in one postmenopausal woman. Their frequency and size increased gradually between groups of pregnant women in the order of non-labor, oxytocin-induced labor and spontaneous labor. The increase in size of the junctions was less than the increase in number. CONCLUSIONS: Gap junctions are most frequently found in human myometrial cells in spontaneous labor, which strongly suggests a role for these structures in the termination of pregnancy. The effect of oxytocin on the appearance of gap junctions was negligible; hence, the contribution of oxytocin to establish myometrial contractile synchronicity is thought to be minor. Caveolae are probably not relevant for the initiation of labor as their distribution did not differ between the groups.

Adolescent↗
PubMed1995

Synthesis of some N,N-disubstituted carbamodithioic acid esters tested for antifungal activity.

The synthesis of 12 new 4-[(N,N-disubstituted thiocarbamoylthio)acetamido]antipyrines and their antifungal activity are reported. Of these compounds, only 3e, showed good antifungal activity against C. tropicalis KUEN 1021, C. krusei KUEN 1001, C. stellatoidea KUEN 1018, C. pseudotropicalis KUEN 1012, C. albicans ATCC 10231 when compared with clotrimazole and miconazole (MIC = 12.5 micrograms/ml).

Antifungal Agents↗
PubMed1995

Sample adequacy in detecting Chlamydia trachomatis.

OBJECTIVE: Chlamydia trachomatis is an important etiological agent in female genital infection and may result in infertility. In recent years rapid diagnostic methods have become widely used as alternatives to cell culture. Our objective was to evaluate the technique of direct fluorescence assay (DFA) in estimating the presence of C. trachomatis. METHODS: Specimens taken from 40 infertile and 20 fertile women were examined by DFA for the presence of C. trachomatis. RESULTS: Six of forty (15%) infertile women were found to be positive whereas no positive specimens were detected in the control group. When the specimens were grouped into those which were adequate or inadequate, 19 and five specimens, respectively, were adequate in the infertile and healthy groups. If only adequate specimens are included in estimating the presence of Chlamydia, the percentage is 31.6%. CONCLUSIONS: In order to use DFA as a more reliable and rapid diagnostic test of C. trachomatis in female genital infection, false-negativity must be eliminated. Specimens must be collected adequately and concentrated in order to achieve optimal diagnostic success.

Cervix Uteri↗
PubMed1994

Prevalence of hepatitis B surface antigen among pregnant women in a low-risk population.

OBJECTIVES: To estimate the prevalence of chronic hepatitis B carriers among our low-risk obstetric population and to determine the need for routine screening. METHODS: A group of 1224 pregnant women, admitted to Gazi University Medical Center, were screened for HBsAg using an enzyme-immunoassay technique, and the obstetrical care records were reviewed. RESULTS: Fifty-three patients were seropositive with a prevalence of 4.33% for HBV chronic carrier status in our obstetric population. CONCLUSIONS: Routine HBsAg screening is advisable in our antenatal population.

Carrier State↗
PubMed1994

Seroprevalence of rubella virus in Turkish pregnant women.

OBJECTIVES: To assess the immune status of a group of Turkish pregnant women who had never been immunized. METHODS: Between June 1990 and April 1993, the seroprevelance of rubella was determined in the study group of 1351 women. RESULTS: A total of 242 (17.9%) pregnant women were found to be susceptible to rubella infection and the seropositivity rate related to prior infection was 82.1%. CONCLUSIONS: The importance of Rubella IgG determination in Turkish pregnant women is discussed and recommendations for prevention and control of congenital rubella syndrome (CRS) are suggested.

Antibodies, Viral↗
PubMed1990

Prevalence of Chlamydia trachomatis infection in the Turkish female population.

Chlamydia trachomatis infection is the most common of the sexually transmitted diseases. In this study of screening for C. trachomatis infection in the Turkish female population, Chlamydia IgG enzyme immunoassay (Ch IgG EIA) was used to detect the exposure to C. trachomatis. Ch IgG EIA was studied in 469 women attending the Gynecology and Obstetrics Clinic of the University of Gazi School of Medicine. Ch IgG EIA was found positive in 35 (7.46%) blood specimens. The highest ratio of seropositivity was detected among the infertile population (11.17%).

Adult↗