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Biomedical subjects

H GREEN

Publications and source records attributed to H GREEN.

At least 55 records · Page 3Linked to original sources
PubMed1959

Effect of antibody and complement on permeability control in ascites tumor cells and erythrocytes.

Rabbit antibody + complement alters the permeability properties of mouse Krebs ascites tumor cells and erythrocytes. When antibody + C' acts on ascites tumor cells in a low protein medium, intracellular K(+) is lost from the cells at a rate far greater than the normal leak rate. At the same time the cells lose amino acids and ribonucleotides and become fully permeable to the Na(+) of the medium. When antibody + C' acts in a low protein medium, the cells swell extensively and lose most of their macromolecules to the medium (hemoglobin from erythrocytes, protein and RNA from the ascites tumor cells). If the antibody + C' acts in a medium containing protein in sufficient concentration to balance the colloid osmotic pressure of the cells, the swelling is prevented; no macromolecules are then lost from the cells, but the loss of K(+) and entrance of Na(+) are not altered, and the loss of amino acids and ribonucleotides is only slightly affected. It therefore appears that the action of antibody + C' is to produce functional "holes" in the animal cell membrane which permit the equilibration of cations and small molecules between cell and medium. This leads to an increase in the osmotic pressure of the cell and a rapid influx of water. The cell membrane and its "holes" are thereby stretched, permitting macromolecules to escape from the cell.

Animals↗
PubMed1959

The cytotoxic action of immune gamma globulin and complement on Krebs ascites tumor cells. I. Ultrastructural studies.

Electron microscopic studies of Krebs ascites tumor cells treated in vitro with rabbit immune gamma globulin revealed a distinctive alteration in the structure of the cellular surface membrane. The subsequent addition of complement to this system severely damaged the cytoplasmic matrix, mitochondria, and endoplasmic reticulum. This sequence of events provides a structural basis for the cytotoxic action of antibody and complement.

Agammaglobulinemia↗
PubMed1959

The cytotoxic action of immune gamma globulin and complement on Krebs ascites tumor cells. II. Chemical studies.

The in vitro exposure of Krebs ascites tumor cells to the action of rabbit immune gamma globulin alone does not result in any changes in the cell concentration of amino acids, ribonucleotides, RNA, DNA, or protein, nor in the rate of entry of potassium into the cell. The exposure of the cells to antibody + complement results in the following changes within a few minutes:- (a) Loss of about two-thirds of the free amino acids and ribonucleotides. (b) Loss of about 90 per cent of the intracellular potassium. (c) Loss of about three-quarters of the cell RNA to the medium, part appearing as TCA-soluble and the rest as TCA-insoluble products. There were no changes detectable in DNA. (d) A small increase in total free amino acid of the cell suspension. (e) Loss of from 30 to 60 per cent of the cell protein. The loss of these substances is believed to occur through a cell membrane which is still intact, as judged by phase and electron microscopy, and still able to discriminate to a small degree against passage of larger molecules.

Agammaglobulinemia↗
PubMed1957

The role of a natural antibody in the rejection of mouse tumor cells by the chick embryo.

Cells of the Krebs ascites tumor of mice grow well in the body of the chick embryo until about the 17th day of incubation, when degeneration of the tumor can be seen in tissue sections and viable tumor cells begin to disappear from the internal organs of the embryo. This death of tumor cells follows the appearance in the chick embryo of serum gamma globulins. Among these are antibodies which can agglutinate the tumor cells in vitro, and destroy their viability. These antibodies occur in the blood without the introduction of any foreign antigen. Their possible origin is discussed. Small numbers of mouse tumor cells growing in the chick embryo are completely eliminated shortly after the time when antibodies ordinarily become detectable. When the number of cells present is larger, viable cells persist longer, and at still higher cell numbers, the embryo or chick is unable to eliminate the tumor, and is itself killed by it. Gamma globulins of older birds injected into young chick embryos bearing growing tumor clear the embryonic organs of viable tumor cells.

Animals↗