Burkitt's lymphoma cells: membrane properties and surface morphology as seen by scanning electron microscopy.
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Biomedical subjects
H Gamliel
Publications and source records attributed to H Gamliel.
Virus-cell interactions as seen by scanning electron microscopy.
Scanning electron microscopy (SEM) has been used during the past few years to determine surface alterations of cells infected with a variety of RNA and DNA viruses. Infection of different cultured cells by RNA and DNA viruses usually revealed dramatic shape changes with surface alterations. The most frequent changes seen were "rounding up" of cells and the development of surface microprojections such as ruffles and microvilli. In the light of available knowledge, suggestions are made in this review for future SEM studies in this field with more sensitive methodologies, which will enable even better characterization of virus-induced cell surface changes.
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Scanning immunoelectron microscopy markers.
Ultrastructural analysis of the cell surface and of membrane components such as antigens or receptor sites by scanning immunoelectron microscopy (immuno-SEM) has been the subject of extensive investigation during the past few years. We review the various immunologic and cytochemical techniques applied to SEM, which have employed latex particles, viruses, bacteriophages, protein A, ferritin, gold granules, hemocyanin and peroxidase as markers, and the advantages and disadvantages of these techniques. From current data, it is clear that immuno-SEM has much to offer in determining the distribution of specific cell surface sites and in positive and unambiguous identification of cell types in heterogeneous cell populations.
Antigen-Antibody Reactions↗
Surface morphology and ultrastructure of isolated hepatic Kupffer and endothelial cells.
Isolated non-parenchymal cells from the rat liver were separated by centrifugal elutriation into two fractions containing structurally intact Kupffer and endothelial cells with purities of over 90% in both fractions. These two cell types were then examined by transmission and scanning electron microscopy. It is concluded that Kupffer and endothelial cells are readily distinguished under the scanning electron microscope on the basis of their different surface features. Kupffer cells show ridges and ruffles while endothelial cells have microvilli and blebs and lack ruffles. As in earlier studies, transmission electron micrographs show that Kupffer cells are larger, have a smaller nucleus--cytoplasm ratio and contain lysosomes while endothelial cells are smaller, have a higher nucleus-cytoplasm ratio and show extensive sieve-plates and fenestrations.
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Myeloma cells: surface morphology as seen by scanning and transmission electron microscopy.
Cells from cultured murine myeloma cell lines and circulating leukemic plasma cells from two patients with generalized myeloma were studied by transmission and scanning electron microscopy. Both circulating and cultured cells exhibited consistent surface architectures. Microvilli and varying numbers of prominent blebs of different sizes were seen. The presence of surface blebs is a characteristic feature of secreting and nonsecreting myeloma cells.
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