Biomedical subjects

H Goos

Publications and source records attributed to H Goos.

26 records · Page 2Linked to original sources

PubMed1987

Influence of verapamil and its combination with glucose-insulin-potassium-infusion on acute myocardial ischemia in dogs.

The influence of verapamil (V) and of V combined with glucose-insulin-potassium (VG) on ischemic injured myocardium was investigated in dogs after ligation of the left anterior descending coronary artery. Three hours after coronary artery ligation with VG application during the last two hours the left ventricular end diastolic pressure (LVEDP) and the pressure rate product were decreased in contrast to the behaviour after V infusion. Contents of ATP and creatine phosphate were preserved in equal extent by V and VG, but the lowest content in inorganic phosphate was found in ischemic and nonischemic left ventricular tissue after VG application. Thus, VG seems to enable the tissue to save more effective energy rich phosphates and to contribute to the economization of cardiac work by reduction of preload.

Adenosine Triphosphate↗

PubMed1986

Beneficial effect of combined glucose-insulin-potassium and mechanical support in acute myocardial ischaemia.

The influence of glucose-insulin-potassium infusion in combination with a mechanical assist device (intraaortic balloon pumping, IABP) on the levels of high energy phosphates in the canine heart after coronary artery ligation was compared with the effect of a separate application of these measures to protect the acute ischaemic myocardium. The combined method normalized the tissue content of creatine phosphate in the nonischaemic tissue contrary to the application of the mechanical or pharmacological assistance alone. In the ischaemic cardiac tissue only the combination of both methods reduced the loss of creatine phosphate, ATP and the sum of adenine nucleotides. With the balloon pumping a reduction in lactate accumulation was achieved, which, however, was significantly lower than that obtained by the combination of heart protective measures: IABP plus glucose-insulin-potassium.

Adenine Nucleotides↗

PubMed1985

Ultrastructural morphometric examinations on the protection provided by prostacyclin against myocardial ischaemia.

Myocardial ischaemia in the dog lasting for three hours is followed by swellings of the mitochondria and dilatation of the sarcoplasmic reticulum. The volume density (Vv) of mitochondria in the peripheral ischaemia zone is 0.2890, that of sarcoplasmic reticulum is 0.0101. Prostacyclin was administered either i.v. or by triggering in conjunction with an intra-aortic balloon pump which led to an increase in the volume density of the mitochondria, mainly as a result of an increase in the volume of average mitochondria. The dilatation of the sarcoplasmic reticulum is reversed. It was not possible to prove in our investigations that prostacyclin provides protection against myocardial ischaemia.

Animals↗

PubMed1984

Improved protection in myocardial ischemia by combined prostacyclin administration and intraaortic balloon pumping.

The influence of prostacyclin (PGI2) alone or in combination with intraaortic balloon pumping (IABP) on the levels of energy-rich phosphate compounds was investigated before and after coronary artery ligation in canine myocardium. There was a higher level in creatine phosphate in the ischemic as well as non-ischemic areas of the myocardium after treatment with PGI2, however the most protective effect was registered after a combination of PGI2 and IABP. PGI2 also reduces the release of cathepsin D activity into the blood independently whether or not a mechanical support of the heart after coronary artery ligation was performed.

Animals↗

PubMed1982

[Improved protection of the myocardium with a combination of intra-aortic balloon counter-pulsation and aimed substrate administration in acute myocardial infarct--a comparative study].

In comparative studies in 36 mongrel dogs it was tried to find out how far intraaortic balloon pumping (IABP) applied solely and in combination with glucose-insulin-potassium (GIK) might influence the degree of acute myocardial ischemia. The ischemia lasted 3 hours. After 60 min myocardial ischemia IABP, the isolated GIK infusion, or the combination of IABP plus GIK were used. The directed GIK application into the aortic root was carried out by electronic triggering during the diastolic augmentation via a precoronary catheter. In the ischemic and in the non-ischemic myocardium of the left ventricle, ATP, creatine phosphate, and lactate were determined. Electronmicroscopical studies were carried out qualitatively as well as quantitatively. The isolated use of IABP suggested no protection of the energy-rich phosphates. The most significant morphological changes were found when exclusively the coronary ligature was applied and when only IABP was used, respectively. The best protective effect was achieved by our technique of the triggered GIK infusion via the precoronary catheter in combination with IABP. The ATP value (n mol/mg w.w.) in the ischemic area was 3.06 and in the non-ischemic one 4.96 in comparison to 1.96 and 3.99, respectively, in the control group. The creatine phosphate (n mol/mg w.w.) was 4.18 in the ischemic and 9.38 in the non-ischemic area in contrast to 2.36 and 6.99, respectively, in the control group. The technique of the triggered drug supply in combination with IABP offers the following advantages: --additive summation of IABP and substrate supply directed to the myocardium --high drug concentration in the myocardium --smaller drug load of the whole organism --clinical use of instable substances.

Animals↗

PubMed1978

[Changes in the excitation contraction coupling in the myocardium of rabbits fed a cholesterol-containing diet].

It was of interest whether a hypercholesterolemia after feeding a diet containing 2 g cholesterol in sunflower oil per day during 12 weeks induces changes of the myocardiac cells of rabbits which must not necessarily be the consequence of a diminished bloodstream on account of coronary stenosis or the consequence of a lesion of the wall of the vessels. The contractile force and the indirectly measured shortening velocity of the isometrically working papillary muscle out of the left ventricle were not reliably changed. As a result of the feeding the isometric force rose after paired pulse stimulation and after a pause during the rhythmic action, although a considerable deposition of fat within the cells and disseminated necrosis in the myocardium could be observed. Because of the increasing loss of sodium into sodium-free solution in parallel with an increasing sodium content of the myocardium it has been concluded that the permeability of the cell membrane for this ion should be elevated. Furthermore it is assumed that the activation of the ionic pumps is elevated which is recognizable from a higher positive inotropic action of both the application of strophanthine and the substitution of NaCl by LiCl. But it seems that one elevation could not completely compensate the other one. The mean value of the velocity of relaxation related to the isometric force was increased. It has been postulated that after the diet rich in cholesterol and sunflower oil the cardiac cell shows a retarded restitution of the contraction during the interval between beats and elevated content of calcium. This may be one of the causes for the observed necrosis besides the ischemic component of the injurant feeding.

Animals↗

PubMed1977

Histological, immuno- and enzyme-histochemical investigations on the adenohypophysis of the urodeles, Mertensiella caucasica and Triturus cristatus and the caecilian, Chthonerpeton indistinctum.

The pituitary glands of two urodelan species (Mertensiella caucasica, Triturus cristatus) and one one caecilian species (Chthonerpeton indistinctum) were examined with histological (Alcian blue, Brookes' trichrome stain), enzyme histochemical (acid phosphatase, alpha-naphthylacetate-esterase, acetylcholinesterase) and immunofluorescence techniques (anti-carp GTH, anti-ovine prolactin, anti-synthetic alpha-MSH). In the pituitary gland of Mertensiella and Triturus six chromophilic cell types could be distinguished. A strong fluorescence was observed in the MSH-, GTH- and TSH-cells. In the pituitary gland of Chthonerpeton only five chromophilic cell types could be distinguished: in the rostral part of the pituitary gland the B3-cell; in the basal region of the central area the B2-cell; dorsocaudally the B1-cell. The acidophilic cells were found in the central and caudal part of the pars distalis. The basophils of the pars intermedia could be observed in the dorsocaudal part of the pituitary gland surrounding the neurohypophysis. All acidophilic cells showed a strong immunofluorescence with anti-ovine prolactin (LTH).

Acetylcholinesterase↗

PubMedDate not supplied

Prostacyclin and iloprost: equal efficiency in preserving high energy phosphates in the dog heart following coronary artery ligation.

The influence of PGI2 and iloprost on the contents of high energy phosphates, cAMP, glycolytic metabolites and on cardiac performance was investigated in open-chest dogs subjected to 3 h of ischaemia, induced by occlusion of the left anterior descending coronary artery. The administration of drugs for 2 h, starting 1 h after the onset of ischaemia, resulted in a normalization and an appreciable reduction in the loss of high energy phosphates in the non-ischaemic and ischaemic left ventricular muscle, respectively, leading to an improvement in the phosphate potential of the cardiac tissue. A reduction in the ischemia-induced rise in cAMP levels was observed, indicating a lower catecholamine overflow in the presence of these drugs. PGI2 and iloprost in the dose employed significantly decreased cardiac preload, which was found to be elevated in the untreated animals after coronary artery ligation. The observed effect of PGI2 and its mimetic on the haemodynamics, as well as on myocardial cAMP and high energy phosphate levels of the heart, may be considered helpful in the phase of temporary imbalance between energy demand and supply during acute ischaemia.

Adenosine Triphosphate↗