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Biomedical subjects

H Grzybek

Publications and source records attributed to H Grzybek.

At least 19 recordsLinked to original sources
PubMed1996

[The use of x-ray microanalysis and transmission microscopy in studies of the chemical composition and ultrastructure of the microregions of precancerous tissues of the larynx].

The authors have presented the results of studies concerning the changes of chemical composition and ultrastructure of tissue microregions at patients with laryngeal precancerous states. Microanalytical studies were made using X-ray JEOL JSM 35 microprobe analyser. The chemical composition of laryngeal precancerous tissue taken from the patients and a control group was estimated. The results of studies were verified using X-ray PHILIPS XL-30 microanalyser. Studies of tissue ultrastructure changes were conducted using JOEL JEM 100 XC transmission microscope. Some heavy metals like, lead, cadmium, nickel, iron, copper and caesium were found in the tissue from larynx precancerous state. Further more, in analysed biologic material there were noticed various degenerative changes within stratified squamous epithelium.

Adult↗
PubMed1993

Transmission electron microscopy of microvilli of intestinal epithelial cells in celiac disease in remission and transient gluten enteropathy in children after a gluten-free diet.

The structure of microvilli of intestinal epithelial cells was investigated in 70 children: 34 with celiac disease in remission, 28 with transient gluten enteropathy after a gluten-free diet, and eight controls. Transmission electron microscopy was used to determine the mean thickness of the glycocalyx layer covering the microvilli, the mean length and width of microvilli, and the number of microvilli per 1 micron length of enterocyte surface. The structure of the glycocalyx was found to be intact, but in some children with treated celiac disease the layer of glycocalyx was either thin or absent on the surface of individual cell microvilli. In children with treated celiac disease, microvilli were statistically significantly shorter than those in children with transient gluten enteropathy and controls. Microvillous width in treated celiac disease was greater as compared with that in controls. There was no difference in the number of microvilli on the enterocyte surface in the three groups.

Biopsy↗
PubMed1993

[Cytoenzymatic and ultramicroscopic investigations of blood in coke oven workers].

White blood cell count, cytoenzymology (acid and alkaline phosphatase, beta-glucuronidase and myeloperoxidase of granulocytes) and ultrastructure of granulocytes and NBT reduction test were performed in peripheral blood obtained from cokery plant workers. All the subjects were divided into groups according to degree of exposure to BaP. Occupational exposure to many factors during coke production, especially to high concentration of BaP cause perceptible changes of NBT reduction test in the group more exposed workers. A statistically significant of the totally activity of the acid phosphatase and beta-glucuronidase of granulocytes were found in this risk group. The changes in granulocyte function correlated with ultrastructural changes. The coking plant environment represent a strong stimulator of the neutrophil metabolism.

Adult↗
PubMed1992

[Morphologic appearance of intestinal microvilli in children with coeliac disease and secondary malabsorption syndromes after gluten-free diet and gluten challenge].

The glycocalyx and the structure of the microvilli were analysed in electron microscopy in 122 children with coeliac disease and secondary syndromes of malabsorption. Slight differences were noted in glycolayx structure between both groups of children. In children with coeliac disease its reduction was noted (after gluten challenge). Changes were observed also in the structure of the microvilli. They were lower and often with abnormal shape in coeliac disease, while they were high and of normal structure in secondary malabsorption syndromes.

Celiac Disease↗
PubMed1991

The small intestinal enterocytes of rats during lead poisoning: the application of the Timm sulphide silver method and an ultrastructural study.

Young male rats were divided into 3 groups which received approximately 100 mg of a lead acetate/kg b.w. per day in their drinking water during 2, 30 and 60 d, respectively. Samples from the jejunum were processed for transmission electron microscopy (TEM), and after 60 d of exposure blocks of tissue were also processed to evaluating the Timm sulphide silver reaction sites in the epithelial absorptive cells in TEM according to Dancher and Zimmer (5). The ultrastructure of enterocytes in poisoned rats at 2 days was similar to the controls. A marked feature of about one third of the rat enterocytes exposed to lead for 30 d was the presence of numerous, small rough-membraned vesicles and prominent, dilated Golgi complexes in their cytoplasm. Most of the enterocytes at the 60th d of lead-exposed rats had a vacuolated cytoplasm associated with the prominent Golgi complexes and vacuoles of various size. They also had pleomorphic rough-membraned vesicles and dilated cisternae of the RER. The presence of the Timm reaction deposits has been observed on the microvillar surface of the brush border in connection with the enterocyte plasma membrane, and in the extracellular space between epithelial cells. Furthermore, Timm precipitates were found in extravascular spaces surrounding capillaries, between endothelial cells, and in the capillary lumen.

Administration, Oral↗
PubMed1991

[Peroxisomes and peroxisomal diseases].

This paper is on peroxisomes ("microbodies") dealing with various aspects of their physiological significance including the central question of biogenesis of peroxisomes. Attention is also focused on how peroxisomes are involved in human pathology and the so-called peroxisomal diseases are described.

Adrenoleukodystrophy↗
PubMed1990

Application on Timm sulphide silver method for electron microscope localization of lead ions in blood cells.

The application of the Timm sulphide silver method for the demonstration of lead ions in peripheral blood cells is investigated, using male white adult Wistar rats treated with a single dose of lead acetate, range 150 mg/kg b.w. To improve the Timm reaction for electron microscopy, fixation of whole cells with glutaraldehyde fixative solution saturated with H2S, an agarose embedding and physical development of thick sections without prior cryostat sectioning are presented. At 6.0 hours after injection both erythrocytes as well as white cells reveal the positive Timm reaction. All types of blood cells contain numerous cytoplasmic precipitates illustrating the intracellular lead accumulation. It is shown that the invaginations of white cell nuclear membrane possess a storing function as areas of lead depots. As a rule, the neutrophils display a highest amount of cytoplasmic precipitates and exclusively a low amount of reaction products in basophils is observed. At 14 days after injection, precipitates are present only in erythrocytes and monocytes. A suggestion of a possible functional significance of changes in the Timm staining pattern in blood cell types is discussed in this paper.

Animals↗
PubMed1988

Ultrastructural aspects of the small intestinal lead toxicology. Part II. The small intestine goblet cells of rats during lead poisoning.

The purpose of the present study was to evaluate the detoxification significance of the small intestinal goblet cells in rats on prolonged treatment with 0.01% lead acetate in the drinking water. These goblet cells were analysed using a submicroscopic demonstration of heavy metals with the sulphide silver method and the X-ray microanalysis probe. Male Wistar rats, weighing 80 g, were sacrificed after 2, 30 and 60 d of exposure to oral administration of lead. The small intestines were perfused with Sörensen buffer with H2S, then blocks of tissue were removed from the border between the duodenum and jejunum and processed for transmission electron microscopy according to Grzybek (3) and Dancher (2). At first the silver containing salt precipitates were distributed in the extracellular space between epithelial cells after 2 d of the experiment. Treatment of rats with lead acetate for 30 d produced the characteristic appearance of the goblet cells at the electron microscopic level. The presence of lead in conjunction with the goblet cell membrane has been observed. If administration of lead to rats is continued longer than 30 d, in the mucus droplets located in the cytoplasm of goblet cells some deposits of silver salts may be demonstrated. For the electron probe X-ray microanalysis the bulk specimens were prepared using a critical point drying apparatus. The specimens were scanned along a line on the villous surface parallel to its long axis. The X-ray studies indicated that accumulation of lead in the epithelial cells increased during the time of experiment.

Animals↗
PubMed1988

Ultrastructural aspects of the small intestinal lead toxicology. Part I: Surface ultrastructure of the small intestine mucosa in rats with lead acetate poisoning.

The effects of low concentration of lead acetate on the apical surface of the jejunal enterocytes were studied. Young male rats were divided into 2 groups which received 0.01% lead acetate solution in drinking water during 30 and 60 d respectively. Blood lead concentrations of poisoned rats were elevated to 30.33 micrograms Pb/100 ml at d 30 of intoxication, then slightly depressed at the end of experiment. Weight gain was impaired only in the 60-d group in comparison with controls. Samples from jejunum were processed for scanning electron microscopy using a critical point drying method and gold evaporation. The fine structure of the surface enterocytes was always determined in the epithelial bands above the levels of crypts nearly half a distance from crypt to villous top. The shape of the jejunal villi in poisoned rats was similar to that in non-poisoned rats. A marked feature of the rats' jejunum exposed to heavy metal for 30 d was a rough appearance of the surface villi, probably associated with distortion of the glycocalyx layer. Extensive areas with degenerative lesions were observed on the surface of the most villi on the 60th d of intoxication. Microvilli of enterocytes distributed within these areas were deformed and sometimes could be completely absent. All enterocytes exhibited various degrees of glycocalyx disturbance. It was concluded that the pronounced toxic effects of lead were related to modification of biochemical properties of the surface coat of enterocytes. This abnormal function of the glycocalyx could result in damage and microvillous malformations.

Animals↗