PubMed Health⌕ Search

Biomedical subjects

H H Fudenberg

Publications and source records attributed to H H Fudenberg.

At least 217 records · Page 12Linked to original sources
PubMed1978

Nephelometric method for determination of rheumatoid factor.

We have developed a nephelometric technique for determining titers of rheumatoid factor. Serum dilutions are added to a mixture of commercially available latex particles of small size (0.098 micrometer) and human gamma-globulin. Rheumatoid sera induce a substantial increase in the light scattering properties of the mixture, and the highest dilution significantly increasing the light scattering is considered as the titer for that serum. This technique is simple to use, provides objective results, and can be automated for analysis of large numbers of samples.

Humans↗
PubMed1978

The distribution of immunoglobin allotypes in two Tlaxcaltecan populations.

The distribution of Glm(f, z, a, and x), G3m(b0, b1, b3, b5, c3, c5, g, s, t and v), A2m(1 and 2) and Km(1) (formerly Inv(1)) allotypic determinants has been examined in specimens from the inhabitants of two transplanted Tlaxcaltecan villages (Cuanalan and Saltillo). The results indicate that Gmza;g Am1, Gmza;g Am2, Gmzax;g Am1, Gmza;bst Am1, Gmza;bst Am2, Gmf;b Am1, Gmza,b Am1, Gmza;b Am2 and Km1 are polymorphic or marginally polymorphic in both populations, while Gmza;bc3,5 Am2, Gmza;bs Am2, and Gmzax;g Am2 were detected only in Saltillo. Two related individuals from Saltillo have either a Gmf;g Am1 or Gmf;-Am1 haplotype while a third unrelated individual had either a Gmf;g Am1 or Gm-;g Am1 haplotype. The frequencies observed for "residents" of Cuanalan are similar to those for other Indian populations in Mexico. Estimation of Caucasian and African admixture within the two communities indicates significant heterogeneity among the inhabitants of Cuanalan, in that Tlaxcaltecan residents have no detectable African admixture and significantly less Caucasian admixture than recent immigrants, with Tlaxcaltecan-immigrant hybrids intermediate, while no significant variation was observed among the subdivisions of Saltillo. However, Saltillo has greater Caucasian and African admixture than Cuanalan. Admixture estimates based on Gm haplotypes appear to agree much better with known historical events than those generated by blood groups, indicating that Gm is a better estimator of admixture than blood groups under certain circumstances.

Black People↗
PubMed1978

Leukocyte migration agarose test (LMAT) in sarcoidosis using Kveim test material.

The two-stage leukocyte migration agarose test (indirect LMAT), shown to be a sensitive in vitro assay for cell-mediated immunity, was used to study Kveim reactivity in vitro in patients with sarcoidosis. No Kveim-induced inhibition of leukocyte migration in agarose or Kveim-induced lymphocyte transformation in vitro was found in 23 patients with sarcoidosis, suggesting that the Kveim reaction is not an expression of cell-mediated immunity.

Adult↗
PubMed1978

Immunoglobulin evolution: chemical study of clawed toad (Xenopus laevis) heavy and light chains.

NH2 terminal amino acid sequence determinations of clawed toad (Xenopus laevis) immunoglobulins indicate that approximately 30% of the heavy chains and less than 5% of the light chains have unblocked NH2 termini. The major amino acid sequence of the X. laevis 7S immunoglobulin heavy chains is the same as that of the 19S immunoglobulin heavy chains. Thus in the synthesis of the heavy chains, the VH genes coding for unblocked heavy chains can associate with CH genes of both the 19S and 7S classes. This association is particularly important in amphibians because, in contrast to mammals and birds, the majority of amphibian antibody-producing cells synthesize both 19S and 7S immunoglobulins and do not participate in the 'genetic switch' characteristic of lymphocyte differentiation in higher organisms. In X. laevis, the major amino acid sequence at the first twenty-four positions of the unblocked heavy chains shows approximately 54% difference from the prototype amino acid sequence of the mammalian VHIII subgroup. Thus, the VHIII gene(s) must have started to appear after the evolutionary divergence of the common ancestor of mammals and birds from the amphibian line. The amino acid composition of the X. Laevis 7S immunoglobulin heavy chains differs from that of its 19S immunoglobulins as well as those of human IgG and IgA. These data support the concepts (a) that amphibian 7S and 19S immunoglobins belong to distinct classes and (b) that amphibian 7S immunoglobulin does not resemble mammalian IgG or IgA.

Amino Acid Sequence↗
PubMed1978

T cells, precocious aging, and familial neoplasia.

A 15-year-old girl presented with precocious aging and was found to have low levels of active and total T cells. Family history revealed a high familial incidence of cancer on both the maternal and paternal sides, and activ T cell levels were found to be low in several living family members. The patient developed osteogenic sarcoma 13 months after initial study. Since our previous studies have reported low active and total T cells in patients with cancer, the present results suggest that subjects with low active T cells should be monitored frequently to detect possible neoplasia in it early stages. They also suggest that impaired cellular immunity in humans is associated with, if not the cause of, accelerated aging.

Adolescent↗
PubMed1978

Studies in sarcoidosis: intradermal Mycoplasma test.

Mycoplasma strain 215-M, closely related to Mycoplasma orale type 1, was grown in human macrophage monolayers in vitro. Sonicated homogenates of macrophages diluted in saline solution were used for intradermal skin tests in 19 patients with sarcoidosis and in 25 patients with a variety of other diseases. Positive skin-test reactions to this newly developed Mycoplasma test material were observed significantly more often in the patients with sarcoidosis than in the other patients.

Adult↗