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H H HALL

Publications and source records attributed to H H HALL.

13 recordsLinked to original sources

STIMULATION OF CAROTENOGENESIS BY MICROBIAL CELLS.

Spent mycelia of Blakeslea trispora recovered from a previous fermentation enhanced carotene production by mated cultures of the same organism. Peak yields of 107 to 142 mg of carotene per 100 ml of medium were achieved in 6 days. beta-Carotene constituted 92% of the total carotenoids produced. The enhancing substance was found present in an aqueous extract of the mycelium. Part of its activity was due to an organic acid fraction. Other microbial cells, molds, yeasts, and bacteria were also capable of enhancing carotene production.

Bacillus cereus↗

Enhancement of beta-carotene synthesis by citrus products.

beta-Ionone, a stimulatory compound in the microbiological production of beta-carotene by mated cultures of Blakeslea trispora, could be replaced with low-cost agricultural by-products (citrus oils, citrus pulp, or citrus molasses) with as good or better carotene yields. Peak yields (81 to 129 mg of carotene per g of dry solids) were achieved in 5 days. The various citrus products tested did not change the pigments produced; all trans-beta-carotene remained the pre-dominant pigment. The acid-hydrolyzed soybean meal and corn used in previous production media could be replaced with unhydrolyzed cottonseed embryo meal and corn in a medium that also contained a natural lipid, deodorized kerosene, nonionic detergent, and a precursor.

Carotenoids↗

Microbiological production of carotenoids. VII. Influence of hydrocarbon on carotenogenesis by mated cultures of Blakeslea trispora.

Synthesis of beta-carotene by mated strains of Blakeslea trispora in shaken-flask culture was considerably enhanced by adding either 5% kerosene after 2 days of fermentation or acid-refined kerosene at the start of fermentation to a grain-based medium that also contained a natural lipid, nonionic detergent, and beta-ionone; average yields of 17,500 mug per g of dry fermentation solids (86,000 mug per 100 ml of medium) were attained when refined kerosene was used. Almost all of the carotene was retained within the mycelium. Peak yields were achieved in 5 days.

Carotenoids↗