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Biomedical subjects

H H Tantawi

Publications and source records attributed to H H Tantawi.

At least 19 recordsLinked to original sources
PubMed1984

Infectious tenosynovitis in broilers and broiler breeders in Egypt.

Two infectious tenosynovitis-producing viruses were isolated from tendon sheaths and synovial fluids of 59 broilers and 15 broiler breeders obtained from different flocks in Egypt during June to October 1983. The viruses grew well on the chorioallantoic membrane of developing chicken embryos, produced small localized white pock lesions with oedematous swellings at the inoculation sites and death of most of the embryos 72 to 96 hours post-inoculation. They also induced cytopathic effect in chicken embryo rough, Vero and MS cell lines. The viruses were neutralized by reovirus S1133 antiserum, both in tissue culture and on the chorioallantoic membrane. Inoculation of the viruses into 2-day-old broiler chicks via the foot pad, intramuscular and oral routes reproduced the disease with the development of characteristic clinical, pathological and serological responses. The infection was transmitted to in-contact control chicks. This is the first report of the disease and of the isolation and identification of the causative virus in Eqypt.

Animals↗
PubMed1983

Susceptibility of day-old chicks and ducklings, goslings and quails to pigeon herpes encephalomyelitis and pigeon herpesviruses.

Day-old chicks were susceptible to pigeon herpes encephalomyelitis virus (PHEV) by intracerebral (i/c) inoculation. Infected birds developed neurologic signs starting from 2 to 15 days post-infection, and 85% died. The virus was recovered from the brains of diseased chicks in titers ranging between 104 and 105.5 EID 50/0.2 ml. Inoculated birds shed the virus in their droppings throughout the 2 weeks observation period. Day-old chicks given the virus by the intranasal (i/n) or oral routes did not develop any specific signs but shed the virus also in their droppings throughout the observation period. Ducklings and goslings inoculated intravenously (i/v), i/n or orally were resistant. Day-old chicks and ducklings, goslings and quails inoculated by different routes with pigeon herpesvirus (PHV) did not show respiratory or nervous signs.

Animals↗
PubMed1983

Avian infectious laryngo-tracheitis in Egypt. I. Epidemiology, virus isolation and identification.

Serious outbreaks of haemorrhagic tracheitis in poultry induced by infectious laryngo-tracheitis virus (ILTV) have been recorded in Egypt for the first time. The disease occurred in different localities during late 1982 and early 1983. The associated drop in egg production ranged between 5% and 35% and there was a mortality rate which ranged from 0.05% to 19.8%. The causal virus was isolated on the chorioallantoic membrane (CAM) of developing chicken embryos where it induced large yellowish-white pock lesions, 3-4 mm in diameter, by the fifth or sixth day post-inoculation. It was non-lethal to the inoculated embryos. It grew also with cytopathic effect (CPE) on the CER cell line. The CPE was characterized by syncytial formation and intranuclear inclusions. Chickens experimentally inoculated with the virus developed respiratory signs and 14 of 20 birds died with subsequent virus re-isolation. The isolated virus was unable to agglutinate chicken red cells and it was precipitated and partially neutralized by reference serum to ILTV. Viral antigen was detected by the indirect fluorescent antibody technique in tracheal smears obtained from naturally and experimentally infected birds. This is the first report of the isolation of ILTV in Egypt.

Animals↗
PubMed1983

Poxvirus infection in a rat (Rattus norvegicus) in Kuwait.

A poxvirus was isolated from skin lesions on the tail of a rat (Rattus norvegicus) in Kuwait in February, 1982. The virus grew on the chorioallantoic membrane (CAM) developing chicken embryos and induced small generalized haemorrhagic pock lesions, 72 hours post-inoculation. It grew also with cytopathic effect (CPE) in CER and Vero cell lines. In cultured cells it produced cell rounding and syncytia 48-72 hours post-infection. The virus was resistant to the effect of ether as well as to chloroform, and showed haemagglutinating activity against chicken red blood cells in a low titer (1:16). It induced in the rabbit skin nodular lesions 4-5 days post-infection. Electron microscopy of the negatively stained preparations revealed brick-shaped typical poxvirus virions measuring 220-250 millmicron in diameter. By using vaccinia antiserum the virus antigen was precipitated in the agargel immunodiffusion test and this indicates its relation to the genus Orthopoxvirus.

Animals↗
PubMed1982

Pigeon herpes encephalomyelitis virus in Egypt.

A virus was isolated in Egypt from brain and liver collected from domestic pigeons suffering from nervous disorders. The morphological and biophysical properties of the virus were consistent with it being a member of the family Herpetoviridae. Antigenically the virus was closely related to if not identical with pigeon herpes encephalomyelitis virus.

Animals↗
PubMed1980

Preliminary characterisation of the Sersenk strain of goat pox virus.

The Sersenk strain of goat pox virus first isolated in Iraq, grew on chorioallantoic membranes (CAM) of developing chicken embryos producing generalised large pocks, 2 to 3 mm in diameter, on the third day post-inoculation. The virus killed the inoculated embryos. Replication of the virus in primary cultures of lamb testis cells induced a cytopathic effect (CPE) and plaque formation characteristic of pox viruses. It was antigenically related to reference strains of goat pox and sheep pox viruses. The virus was sensitive to ether and chloroform, failed to agglutinate erythrocytes and was strictly pathogenic to goats.

Animals↗
PubMed1980

Crimean-Congo haemorrhagic fever virus in Iraq: isolation, identification and electron microscopy.

Crimean-Congo haemorrhagic fever (CCHF) virus was isolated for the first time in Iraq from the blood of three patients. It caused a cytopathic effect in lamb kidney and BHK-21 cell cultures. The virus particles were spherical, enveloped and had 90 nm in diameter similar particles were found in ultrathin sections of the liver from two fatal cases. The isolated virus proved to be antigenically closely related to CCHF virus.

Animals↗
PubMed1979

Isolation and identification of the Sersenk strain of goat pox virus in Iraq.

Goat pox virus was isolated during an outbreak of pox infection among goats in the Sersenk district, Iraq. The isolated virus grew on the chorioallantoic membranes of developing chick embryos and in primary lamb testis cell cultures. It was identified morphologically as a pox virus and serologically as a member of the Capripoxvirus group in the family Poxviridae. The isolated virus was designated the "Sersenk" strain.

Animals↗
PubMed1979

Isolation, identification and characterization of camelpox virus in Iraq.

A virus was isolated from pox-like lesions in camels during an outbreak of camelpox disease which occurred in December 1977 in an area near the Iraqi-Iranian border. It was identified serologically as a virus of the Orthopoxvirus group. The biological properties of the isolate indicated that it was probably identical with strains of camelpox virus isolated from Iran, Egypt, Kenya and the U.S.S.R.

Animals↗
PubMed1979

Pox infection among captive peacocks.

An outbreak of avian pox was detected among captive peacocks (Pavo cristatus) at Baghdad Zoological Park during spring, 1978. A total of 45 of the 60 birds in the aviary developed pox lesions around the beaks and eyes. Morbidity was 75% and mortality was 13%. A virus isolated from the skin lesions produced large plaques on the chorioallantoic membrane of developing chicken embryos and induced cytopathic effect characteristic for pox viruses in chicken embryo cell cultures. The virus neither haemagglutinated nor haemadsorbed to chicken erythrocytes. It was ether resistant and chloroform sensitive. Chickens inoculated with the virus by scarification developed localized pox-like lesions, while turkeys had only transient swelling of feather follicles at the site of inoculation. Virus partially purified with Genetron 113 was precipitated by antisera to fowlpox and pigeon pox viruses.

Animals↗
PubMed1979

Heat-selected mutants of pigeon pox virus.

Two mutants of pigeon pox virus were derived from virus isolated from naturally infected pigeons. One (S 39) was obtained by cultivation of the original virus in chick embryo chorioallantoic membranes at 39 degrees C, and the second (S m) by heating the original virus at 56 degrees C for 30 min. The mutants were less pathogenic to pigeons than the original virus. The original virus and the mutant S 39, but not the mutant S m produced plaques in primary chick embryo cell cultures. Pigeons inoculated with the mutants were resistant to challenge with the field virus.

Animals↗
PubMed1979

Laboratory characteristics of four strains of goatpox virus.

Significant differences were found between four strains of goatpox virus isolated from goats in Egypt, Iraq, Iran and the U.S.S.R., namely in their growth behaviour in embryonated eggs, ceiling temperature, plaque characteristics in cell culture, heat resistance and in the degree of inhibition of their replication by actinomycin D and bromodeoxyuridine. All the strains studied had morphologically similar virions and induced type A V- inclusions. They were uncapable to produce haemagglutinins in infected cell cultures. Antigenically, the strains were indistinguishable.

Animals↗
PubMed1978

Comparative studies on poxvirus strains isolated from camels.

Five poxvirus strains isolated from camels in Egypt, Iran and the U.S.S.R. were investigated. The morphology and size of the negatively stained virions of the investigated strains were identical. The strains produced minute white pock lesions localized mainly on the infected area of the chorioallantoic membrane, without generalization of lesions or death of embryos. The ceiling temperature and the haemagglutinating activity of the different strains were different. All the strains were apathogenic for rabbit skin and highly pathogenic for mice inoculated intracerebrally. Serologically, all the strains were related to vaccinia virus but with different degrees. The studied strains had the characteristics of members of the genus Orthopoxvirus.

Animals↗
PubMedDate not supplied

Viral encephalomyelitis of pigeons: pathology and virus isolation.

A previously unrecorded nervous disease in pigeons was investigated. The disease, characterized by paresis, paralysis of the extremities, head-shaking, and torticollis, is contagious and spreads slowly. The mortality rate of affected pigeons was very high. The disease appeared to spread among pigeon flocks in spring and summer. The predominant gross change in most cases examined was congestion of the visceral organs. Some cases had grayish spots on the pancreas and kidneys. The histologic changes are characterized by neuronal and myelin degeneration with mononuclear cell infiltration and perivascular cuffing. Degeneration of the parenchyma and marked congestion are prominent in the visceral organs. The causal agent, found to be a virus, produced pock lesions on chorioallantoic membranes of developing chick embryos and failed to aagglutinate chicken RBCs. Antisera against Newcastle disease virus and avian encephalomyelitis virus did not neutralize the isolated virus. The virus produced typical signs in experimentally inoculated pigeons.

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