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Biomedical subjects

H Haase

Publications and source records attributed to H Haase.

At least 91 records · Page 5Linked to original sources
PubMed1982

Potentiating effect of calmodulin and catalytic subunit of cyclic AMP-dependent protein kinase on ATP-dependent Ca2+-transport by cardiac sarcolemma.

Highly purified sarcolemmal membranes were prepared from pig heart homogenates by differential and density gradient centrifugations. The membrane fragments exhibit ATP-dependent Ca2+-transport and Na+/Ca2+-exchange activities. ATP-dependent Ca2+-transport (K0.5Ca2+ = 0.3 microM; Vmax = 4.6 nmol Ca2+.mg protein-1.min-1) is not stimulated by oxalate. Ca2+-uptake is also not supported by p-nitro-phenylphosphate. Preincubation of sarcolemma with MgATP, calmodulin and catalytic subunit of cyclic AMP-dependent protein kinase stimulates active Ca2+-transport 1.8-fold. The effects of calmodulin and catalytic subunit are potentiating rather than additive. A large portion of the Ca2+ additionally accumulated after prephosphorylation of membranes is exchangeable for Na+ via the Na+/Ca2+-exchange system.

Adenosine Triphosphate↗
PubMed1982

[Results of endocrinolgic studies of the 3rd international crew of the scientific orbital station complex; Soyuz 29 - Salyut 6 - Soyuz 31 (joint space flight enterprise of the USSR - GDR). 2. Hormones and biologically active substances in blood].

Cortisol, insulin, somatotropin, thyreotropin, thyroxine, triiodothyronine, testosterone, aldosterone, c-AMP, c-GMP, prostaglandins (PGF1-x, PGF2-x, PGA + E), and renin concentrations in serum or plasma of the venous blood of the third international crew of the scientific orbital complex of "Soyuz 29 - Salyut 6 - Soyuz 31" were determined following the 7-day space flight. The increased activity of the renin-angiotensin-aldosterone system before the flight as well as variations in the pressor/depressor prostaglandin ratios indicate an increased strain during the pre-flight period. During the first stage of the post-flight period some parameters were changed due to the landing process and the returning to earth gravity. The associated physical load and the onset of reactions for enhancement of the orthostatic tolerance resulted in an increase of cyclic nucleotid and thyroxine concentrations. The relatively higher levels of the pressor PGs of group F in comparison with the prostaglandins A + E could be evaluated as a compensatory reaction for enhancement of the orthostatic tolerance. The cortisol and STH concentrations increased with growing motor activity. The variations seen after the 7-day space flight were essentially within the reference areas. It may be assumed that the readaptation was not yet totally accomplished by the 8th day after landing.

Endocrine Glands↗
PubMed1982

[Results of endocrinologic studies of the 3d international crew of the scientific orbital station complex: Soyuz 29--Salyut 6--Soyuz 31 (joint space flight enterprise of the USSR--GDR). 1. Condition of the sympathico-adrenal system].

The present paper deals with the activity of the sympatho-adrenal system (SAS) in the third international crew of the scientific orbital station "Salyut-6" on the basis of catecholamines (CA) as well as of some of their primary stages and metabolites. Plasma adrenaline (A), noradrenaline (NA), and dopamine (DA) were determined on the 30th day before the flight and on the first day after landing. The parameters of A, NA, DA, DOPA, metanephrine (MN), and normetanephrine (NMN) as well as their conjugates were analysed in urine on day 30 and from days 5 to 1 before launching the spaceship and from days 0 to 5 after landing. Moreover, at the same occasions the urinary excretion of vanillilmandelic acid (VMA) and homovanillic acid (HVA) was measured. For a qualitative evaluation of the responses of SAS indices were computed providing information on the activity of the single links of CA metabolism. During the last five days before launching a gradual increase of A, NA, DA, and DOPA excretion with the urine was found in both the cosmonauts. The A/NA quotient rose to 360% in the commander and to 200% in the spacecraft engineer. The inactivation processes of A (MN/A) and NA (NMN/NA) were clearly reduced in both crew members before launching. The highest excretion of A, NA, DA and their conjugates as well as of DOPA was observed on the day of landing. The total results ascertained in the post-flight period indicate psychonerval strains experienced by the cosmonauts. However, marked stress reactions were not found. This conclusion is drawn on the basis of general criteria of the indices of CA metabolism during stress (increased secretory activity of SAS and excretion of free CA, synthesis activation, relative hypometabolism of conjugation). The rapid normalisation of the investigated parameters after the flight gave evidence of the cosmonauts' quick readaptation, which again is an expression of a high state of preparatory training.

Adrenal Glands↗
PubMed1982

[Results of endocrinologic studies of the 3d international crew of the scientific orbital station complex: Soyuz 29 - Salyut 6 - Soyuz 31 (joint space flight enterprise of the USSR - GDR). 3. Corticosteroid excretion in urine].

In the present study the attempt was made to evaluate the functional state of the adrenal cortex after space flight by means of the urinary corticosteroid content. The urine collected over 24 hours was analysed. The following parameters were determined: Total 17-hydroxycorticosteroids (17-OHCS), their free forms, glucuronides and sulphates; cortisol (F), cortisone (E), corticosterone (B), 11-desoxycortisol (S), 11-dehydrocorticosterone (A), tetrahydrocortisol (THF), tetrahydrocortisone (THE), tetrahydrocorticosterone (THB), tetrahydro-11-desoxycortisol (THS), and aldosterone. In order to characterize the activity of the 11-hydroxylase the quotient of THS + S/THF + F + THE + E was formed. The activity of the 17-hydroxylase was determined indirectly by the quotient of THF + THE + THS + F + E + S/THB + B + A = 17-OHCS/17-DOHCS. The expected distinct increase of the 17-OHCS after the space flight failed to appear. This finding may be due to an adrenocortical dysfunction. In the space ship commander the quotients indicate a reduced 11- and 17-hydroxylation during the process of synthesis of the glucocorticoids. In the flight engineer they give evidence of an unchanged biosynthesis except on the day of landing. A hitherto not fully intensified substance ("white spot") was found in the chromatogram of the space ship commander on the first and second day after the flight. Moreover, the investigations show that the reaction of the adrenal cortex after space flights exhibits a number of individual characteristics.

Adrenal Cortex Hormones↗
PubMed1981

Measurement of the partial oxygen pressure and oxygen utilization in the skin of cosmonauts aboard Salyut 6.

The oxygen tension (PO2) in the dorsal skin surface of the forearm was studied during the stay of cosmonauts on board Salyut 6. Between the fourth and fifth day of stay on the orbital station a considerable reduction of the PO2 level was observed. The oxygen utilization values were also reduced. In the early postflight period the low PO2 level persisted with gradual normalization.

Aerospace Medicine↗
PubMed1979

[Studies of necrotizing enteritis of suckling piglets (Cl. perfringens typc C enterotoxemia) in industrialized sow breeding units. 3. Experimental reproduction of the disease].

Experimental reproduction of necrotising enteritis of sucking pigs was successfully achieved by using both Clostridium perfringens Type C strains, which had been isolated from sucking pigs with necrotising enteritis, and Type C strain 3628 of A.T.C.C. (sub-type C1). The lethal dose for sucking pigs was between 20 X 10(6) and 12 X 10(7) pathogens per animal. The disease could not even be induced by repeated application of no-bacterial toxin of Cl. perfringens Type C nor by administration of Cl. perfringens Type A strains which had been cultured from broilers with necrotising enteritis. Necrotising enteritis was found to develop in two phases in sucking pigs. First, the pathogen will deposit to the villous epithelium and then penetrate the superficial strata of the mocous membrane. In the second phase, the villous structure will be destroyed by the lethal, haemolysing, and necrotising toxins of Cl. perfringens. The role played by individual toxin fractions is discussed together with the importance of humoral and localised infection defence. Sucking pigs may be sufficiently protected against infection based on single or ten-fold lethal infectious dosage by two vaccinations of the mother animal, five and three weeks prior to parturition, using "Enterotoxaemia Vaccine Dessau bivalent". Infection then would not occur unless a hundredfold lethal dose was applied. Characteristics include diarrhoea, apathy, exhaustion, and death.

Animals↗
PubMed1977

[Automatic data processing for organisational aid to blood donor centers in emergency hospitals].

A test model of a computer-off-line system at the Community Hospital Blood Bank in Herford collecting approximately 3.000 units of blood annually is described. The computer system presented, now in operation for more than one year, has proven its value for general blood bank administration by its time-saving effect and the optimalized documentation of donor- and blood-related data. The computer system described is particularly recommended for smaller, hospital-based blood transfusion services. Its introduction is even justified in institutions preparing less than 5.000 units of blood per year. Prerequisite for an economical operation of the computer system is the participation of the hospital management in other computer activities, i.e. book-keeping, accounting etc.

Blood Banks↗