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Biomedical subjects

H Harke

Publications and source records attributed to H Harke.

9 recordsLinked to original sources
PubMed1992

Attachment of a single fluorescent label to peptides for determination by capillary zone electrophoresis.

Complicated electropherograms are produced in the separation of fluorescently labeled peptides. Incomplete labeling of epsilon-amino groups on lysine residues results in the production of 2n-1 reaction products, where n is the number of alpha and epsilon amino groups in the peptide. A single label is attached to the peptide by first taking the peptide through one cycle of the Edman degradation reaction. All epsilon-amino groups are converted to the phenyl thiocarbamyl and the cleavage step exposes one alpha-amino group at the N-terminus of the peptide; the fluorescent label is attached to the N-terminus.

Amino Acid Sequence↗
PubMed1979

[The treatment of haemorrhagic complications due to disturbance of platelet function after operations with extracorporal circulation (author's transl)].

The preoperative selective blood cell separation followed by the postoperative platelet autoransfusion could prevent the exhaustion of platelet function by operations with extracorporal circulation. The postoperative blood loss could be reduced by about 57%. This method can be recommended specially in cases of increased platelet traumatization after long time perfusion. Partial disturbances of platelet function after operations with ECC can also be favourably influenced by infusion of homologous phospholipid (Fibraccel). The postoperative blood loss could be reduced by about one third. This method is beneficial in open heart surgery with short perfusion times due to its little technical expenses.

Aortic Valve↗
PubMed1979

[The therapeutic use of streptokinase in the treatment of shock lung (author's transl)].

After protracted haemorrhagic or traumatic shock, the danger of severe respiratory insufficiency is often very imminent. This serious complication is frequently acutely progressive. Even the early use of adaequate artificial respiration cannot always prevent the developement of shock lung. The significance of the different pathogenic factors is still not definitely clear. The main changes that are most imminent during shock are the organisation and carnification of cells and plasma proteins within the lung parenchyma due to increased extravasation. On the basis of pathophysiological considerations however, it seems reasonalbe to interrupt these reactions through early use of monophasic proteo- and fibrinolysis. The indication for and prognosis of a monophasic streptokinase activated fibrinolytic and proteolytic therapy was first examined in 18 patients with a clinically manifest shock lung. In 11 patients the severe initial pulmonary changes could be reversed within 48-60 hours after the beginning of treatment. The normalization of lung function was confirmed clinically and by means of x-ray and blood gas analysis. In 7 patients in which acute renal failure had occurred the progression of pulmonary failure could not be interrupted. The present study demonstrates for the first time the possibilities of fibrinolytic treatment of the shock lung. The risk of haemorrhagic complications especially in the immediate postoperative or post traumatic phase could be limited by a high dose streptokinase therapy. Under these condition the formation of excessive amounts of free plasmin in the circulation was prevented and the so-called endogenous lysis was primarily achieved.

Acute Kidney Injury↗
PubMed1977

[Effect of a preoperative separation of platelets on the postoperative blood loss subsequent to extracorporeal circulation in open heart surgery (author's transl)].

After operations with extracorporeal circulation there is a risk of clotting disorders, due to traumatisation of blood. The extent of cell damage are shown, in particular, by qualitative and quantitative impairment of platelet function. The clinical application of modern blood processors offer the possibility of selecting platelets from patients blood in the immediate preoperative period. Open heart operations were preceded by separation of platelets in 17 patients using a Haemonetics blood processor. After a postoperative retransfusion of the platelets an evident improvement in platelet function and a significant decrease in the predisposition to bleeding was demonstrated in the further postoperative period. During and after extracorporeal circulation the extent of microembolisation was registered by screen filtration pressure. In the clinical experiments, regularly, there is a significant increase of screen filtration pressure in the immediate postoperative period. These reactions were not seen in patients in whom preoperative separation of platelets was carried out. The most important clinical advantage of preoperative plateletpheresis consists in a significant decrease of postoperative blood loss. In particular the development of postperfusion lung will be prevented.

Blood Platelets↗
PubMed1976

[Prevention of microaggregation in stored blood (author's transl)].

Microembolism is still a feared complication during and after blood transfusions. Up to now they could only be diminished by special filter systems. In spite of much research it has not been possible as yet to fine adequate remedies for the preservation of blood cells and for the decrease of aggregation in stored blood. As foregoing studies have shown the inhibition of aggregation is combined with a considerable impairment or even loss of platelet function. In these clinical experiments, however, it has been shown, that aggregations in stored blood are significantly stunted by early addition of Aprotinin. It is clinically important, that no inhibition of platelet function occured.

Aprotinin↗
PubMed1976

[The influence of different plasma substitutes on blood clotting and platelet function during and after operations (author's transl)].

It has been shown in clinical experiments that plasma substitutes generally have a specific-colloidal influence on special parts of the clotting system. These changes are predominant after infusion of dextran, since on one hand there is an inhibition of platelet function, and on the other hand a decrease in the activity of plasmatic clotting factors. It is to be emphasized that hydroxyethyl starch and with some restrictions even gelatins cause a comparable inhibition of platelet function. These reactions are due to colloid-specific effects only, which have been unknown up to now. Activities of plasmatic clotting factors, however, are not influenced by these substitutes.

Blood Coagulation↗
PubMed1975

[Thrombosis of the Björk-Shiley valve as an early fatal complication of the prothetic aortic valve replacement (author's transl)].

From a series of 28 patients subjected to aortic valve replacement with the Björk-Shiley tilting disc valve prosthesis 3 cases are reported in which massive thrombosis with valve dysfunction and fatal outcome occurred in the very early postoperative period. The autopsy findings and the examination of the blood coagulation state during the first 24 hours postoperatively disclosed in all 3 cases a hypercoagulability being responsible for the massive clot formation. The early recognition and establishment of the diagnosis of a hypercoagulability state and an immediate anticoagulant therapy with heparin are necessary for preventing such early fatal complications in patients with cardiac valve replacement.

Adult↗
PubMed1975

[The influence of increased clotting reactions as shown by thrombosis formation in the immediate postoperative period on aortic valve prothesis (author's transl)].

Massive thrombosis formation on the valve periphery and on the top surface of the valve occurred in three patients (1,5%) in the immediate postoperative period with the Björk-Shiley valve in the aortic position. Between the third and sixth day these patients died of acute heart failure as a result of coronary artery displacement. Upon autopsy operative technical complications and postoperative infections were ruled out as the cause of death. What appears to be clinically important is an increase in clotting time in the immediate postoperative period which can be proven statistically. This increased clotting inclination was only found in these three patients and in one patient with frequent immediate postoperative peripheral embolic episodes. We therefore feel that early anticoagulation therapy is necessary. Heparin administration is preferred as it not only lowers the clotting ability of the blood but also the adhesive quality of the platelets.

Aortic Valve↗