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Biomedical subjects

H Harrer

Publications and source records attributed to H Harrer.

12 recordsLinked to original sources
PubMed1988

A new recombinant procoagulant protein derived from the cDNA encoding human factor VIII.

We have constructed new B domain deletion derivatives of human factor VIII (FVIII) by manipulating the cDNA using recombinant DNA techniques. One of these new derivatives, FVIII delta II, in which amino acids 771(pro)-1666(asp) have been deleted, no longer contains the protease cleavage site at amino acid position 1648(arg)-1649(glu) known to be involved in the initial step of FVIII processing. We have expressed this molecule in both baby hamster kidney (BHK) 21 cells using the vaccinia virus (VV) expression system and have established Chinese hamster ovary (CHO) derived permanent cell lines expressing either recombinant (r)FVIII or FVIII delta II. The characteristics of FVIII delta II have been compared to those of rFVIII and/or plasma derived (pd) FVIII. FVIII delta II has the following properties: (i) it exhibits FVIII procoagulant activity; (ii) it is expressed at 5-fold higher levels than is the complete molecule in comparable systems; (iii) it migrates for the most part as a single major band on SDS-PAGE, in contrast to the complete molecule; (iv) it is activated to a greater extent by thrombin than is either rFVIII or pdFVIII; and (v) it retains the ability to bind von Willebrand factor (vWf).

Antibodies, Monoclonal↗
PubMed1987

Two independent domains of factor VIII co-expressed using recombinant vaccinia viruses have procoagulant activity.

Using recombinant DNA technology, the NH2 and COOH terminal domains of the human Factor VIII molecule were co-expressed in baby hamster kidney 21 (BHK21) cells using the vaccinia virus system. Procoagulant activity was detectable in cell supernatants, thus suggesting that the central portion present in the FVIII protein (domain B) is not required for FVIII function.

Animals↗
PubMed1985

[Multiple sclerosis and the electroencephalogram (computer EEG studies)].

A computerized analysis of EEg-records from 96 multiple sclerosis (MS) patients yielded about twice as many abnormal results (79%) in comparison to the usual visual EEG-evaluation (39%). The statistically significant (p less than 0.05) increase in slow frequencies in addition to beta frequencies to the cost of alpha, but especially the reduction in total percentage of alpha contribution to overall EEG-power discriminated most efficiently between MS-patients and our normal population. Thus, alpha index alone allowed for 87% correct classification of the normal population, and 73% of patients with normal visual EEG-records were correctly classified as belonging to the MS-population. Extending the patient group to include pathological visual EEG-records as well, the corresponding figures were 95% and 89%, respectively. The computerized EEG-analysis was thus clearly superior to the usual visual inspection.

Adult↗
PubMed1980

[Autonomic dysfunction in multiple sclerosis (respiratory and cardiac disturbances) (author's transl)].

Except for rectal, vesical and sexual disorders almost nothing is known about disturbances of autonomic functions in multiple sclerosis. MS plaques, however, do not spare vegetative centres and spinal tracts. Therefore autonomic disorders are to be expected in other functional ranges as well. We could not confirm the study of Neubauer and Gundersen (1978) who found the short time variability and the long time variability of pulse rate to be significantly diminished in patients suffering from multiple sclerosis. Our investigation, however, revealed frequent occurrence of rigid, paradoxical and atactic courses of the patients' graphs obtained by "averaging" the pulse rate alterations dependent on respiration. We consider this to be an indication of cardio-respiratory hyposynergia in precision regulations. As compared with the normal control group the patients' respiratory rate was significantly raised, and this was almost exclusively due to an abbreviated time of inspiration. Concerning the pulse rate and the pulse-breathing quotient the patients did not differ significantly from the healthy controls. The findings in 40 MS patients (definite diagnosis) and in 40 healthy persons point to the fact that cardio-respiratory disorders are no rare occurrence.

Adult↗