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Biomedical subjects

H Hayes

Publications and source records attributed to H Hayes.

At least 19 recordsLinked to original sources
PubMed1995

Partial nucleotide sequence and chromosomal localization of a bovine zinc finger gene ZNF164.

A clone carrying an open reading frame coding for a novel zinc finger protein of the Krüppel family was isolated from a bovine genomic library and designated ZNF 164 (zinc finger protein 164). Partial sequencing revealed that it contained at least 13 zinc finger motifs preceded by a lysine-rich region of 60 amino acids. The ZNF164 protein shared approximately 60% similarity with several zinc finger proteins but did not appear to be orthologous with a previously identified gene. Using fluorescence in situ hybridization, the ZNF164 gene was mapped to bovine chromosome band 17q24.

Amino Acid Sequence↗
PubMed1995

Identification of p and q arms of the blesbok (Damaliscus dorcas phillipsi, Alcelaphinae) RBG-banded chromosomes with comparison to other wild and domestic bovids.

The RBG banded karyotype (2n = 38) of the blesbok (Damaliscus dorcas phillipsi) comprises 8 pairs of metacentric, 3 pairs of submetacentric and 7 pairs of acrocentric autosomes. The X and Y chromosomes are acrocentric. The 11 pairs of biarmed chromosomes correspond to Robertsonian translocations involving the chromosomes equivalent to goat and cattle chromosomes: 1;10, 4;5, 8;17, 7;9, 6;14, 13;15, 12;16, 20;22, 3;19, 2;27 and 11;23. The comparison of Blesbok karyotype with that of other species of Alcelaphinae and Hippotraginae reveals a high level of homoeology.

Animals↗
PubMed1995

Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes.

Commercially available human chromosome-specific DNA libraries covering the whole karyotype were hybridized to normal bovine metaphase spreads to characterize the conserved chromosomal segments between man and cattle. All chromosome libraries except the Y chromosome library displayed a signal on at least part of one or more bovine chromosomes. The labeling was clearly visualized and permitted precise delineation of the hybridized bovine chromosomal segments. This study indicates that the reorganization of the genetic material between human and bovine genomes is not as great as expected from classical comparative cytogenetics based on banding patterns. However, apart from interchromosomal rearrangements between ancestral forms of human and bovine chromosomes, a majority of intrachromosomal rearrangements must have occurred in these species during evolution to explain the differences in the banding patterns of their chromosomes. These results show that chromosome painting with heterologous chromosome-specific DNA libraries can provide useful information in comparative studies on karyotypes and gene maps of distantly related mammalian species. The observations are discussed in relation to published data on gene mapping in man and cattle.

Animals↗
PubMed1994

The C-, G-, and R-banded karyotype of the scimitar-horned oryx (Oryx dammah).

Karyotypes of five males and one female scimitar-horned oryx (Oryx dammah) were prepared using lymphocyte and skin cells. CBG-, GTG-, and RBG-banded chromosomes were analysed and the banding patterns were compared with those of cattle, revealing a high level of homoeology except for chromosomes 9, 14, 21, Y, and X. Standard karyotypes are proposed for the scimitar-horned oryx karyotype, which contains 58 chromosomes with one pair of large submetacentric autosomes and 27 acrocentric autosomal pairs. The X and Y chromosomes are the largest and smallest acrocentrics. The scimitar-horned oryx chromosome 1 is submetacentric and appears to result from the fusion between ancestral forms of bovid chromosomes numbered 1 and 29 according to the READING CONFERENCE (1980) nomenclature and to ISCNDA 89 if GTG-banding is used.

Animals↗
PubMed1993

DSM-III-R disorders, social factors and management of school attendance problems in the normal population.

Eighty 13-15-year-old children who failed to attend one of four schools for more than 40% of a term, without good reason, were studied. A systematic schedule (C.A.P.A.) was used in interviewing parents and children. Twenty-five had DSM-III-R Disruptive Behaviour Disorders and 15 had Anxiety/Mood Disorders. Truancy was associated with the former and school refusal with the latter but both often occurred without any Disorder. Fourteen children had neither school refusal nor truancy. Compared to controls, poor attenders came from materially disadvantaged homes. School refusal with anxiety disorders rarely received psychiatric treatment. Non-disturbed absentees were not usually dealt with appropriately.

Absenteeism↗
PubMed1992

Chromosomal localization of the ovine beta-casein gene by non-isotopic in situ hybridization and R-banding.

The ovine beta-casein gene (CNS2) has been mapped to a specific chromosome band using nonradioactive in situ hybridization and simultaneous fluorescent R-banding. The probe pTZ-E4 was a fragment of the ovine beta-casein gene inserted in the plasmid pTZ18R and labeled with biotin-11-dUTP. It hybridized to band q32 of ovine chromosome 4. The discrepancy between this result and the previous localization of this gene on cattle chromosome 6 may be explained by the very great similarity of the banding patterns of ovine and bovine chromosomes 4 and 6.

Animals↗
PubMed1991

Monoclonal antibodies specific for human chromosome 5 obtained with a monochromosomal hybrid can be used to sort out cells containing the chromosome with a FACS.

Using a human-mouse monochromosomal hybrid, BG15-6, that contains an intact human chromosome 5, we isolated four monoclonal antibodies, 2A10, 3H9, 5G9, and 6G12, as chromosome marker antibodies recognizing cell surface antigens specific for human chromosome 5. The binding patterns of these antibodies to BG15 subclones containing fragments of human chromosome 5 indicated that 2A10, 3H9, and 6G12 recognized the antigens produced by genes located on 5pter-q22, and that 5G9 recognized the antigen produced by a gene located on 5q23. Cells containing human chromosome 5 were very effectively sorted in a fluorescence-activated cell sorter (FACS) using monoclonal antibody 6G12. This method for sorting cells containing human chromosome 5 or an appropriate fragment of this chromosome from among human-rodent hybrid cells should be very useful in studies on gene expression, gene cloning and gene mapping.

Animals↗
PubMed1991

Comparison of RBG-banded karyotypes of cattle, sheep, and goats.

The karyotypes of the three main domestic Bovidae species. Bos taurus, Ovis aries, and Capra hircus, have been investigated by RBG-banding. Primary fibroblast cells were cultured from fetal lung or muscle tissue and collected for chromosome preparation after double thymidine synchronization. Depending on the culture and species, BrdU and FdU were added for 8-11 h or 7-10 h, respectively, before harvest. The chromosomes of the three species were classified and numbered according to standard conventions (ISCNDA, 1989) and compared at different stages of condensation. Within the resolving power of the images obtained, the RBG-banding patterns appear to be very similar, with only minor differences existing between chromosome 9 and the sex chromosomes of cattle and the same chromosomes in the domestic sheep and the goat.

Animals↗
PubMed1988

Mammography and breast implants.

Recent recommendations by the American Cancer Society have focused attention on the value of screening mammography in the detection of occult breast cancers. This has resulted in a proliferation of "walk in" and mobile mammography screening clinics and a barrage of publicity aimed at women aged 40 and over. Among these are more than a half million women who have had an augmentation mammaplasty; at least another half million are still under 40 but entering this age group incrementally. Opinion is divided as to the value of this procedure because of uncertainty as to the amount of breast tissue obscured by the implant. Calibrated planimetry was used to measure the area of the implant and the glandular portion of the breasts in six sets of mammograms. Utilizing solid geometric calculations, it was found that the percentage of glandular tissue obscured by the implant varied from 22 to 83 percent. This wide variation casts serious doubt on the reliability of routine film screen mammography in these patients.

Breast↗
PubMed1987

Regional assignment of the gene for diphtheria toxin sensitivity using subchromosomal fragments in microcell hybrids.

Human x mouse microcell hybrids resistant to G418 were constructed between mouse hepatoma cells and human x mouse whole cell hybrids containing only intact human chromosome 5 and 22 with an integrated neor-gene. Among these, microcell hybrid BG15 produced four subclones, BG15-4, BG15-6, BG15-7 and BG15-9, which contained variously sized complements of human chromosome 5. BG15-6 contained an intact human chromosome 5, BG15-7 a deleted human chromosome 5 (5pter-q22) and BG15-4 and BG15-9 a translocation between parts of human chromosome 5 (pter-qter? and pter-q23, respectively) and a mouse chromosome. Southern DNA blot analysis showed that the human dihydrofolate reductase (DHFR) gene was present in all four subclones, whereas the human homolog of the v-fms gene was present in BG15-4 and 15-6, but absent from BG15-7 and 15-9. BG15-4, 15-6 and 15-9 were sensitive to diphtheria toxin, and only BG15-7 was resistant to the toxin. We used these microcell hybrids to restrict further the regional location of the gene for diphtheria toxin sensitivity to the q23 region of human chromosome 5.

Animals↗
PubMed1987

Regional assignment of five genes on human chromosome 19.

A human-mouse hybrid segregant HM76Dd40-6 with new characteristics was derived from the hybrid cell line HM76Dd containing human chromosome 19 as the only human chromosome. Three virus sensitivities located on human chromosome 19 (PVS, E11S and RDRC) were lost in HM76Dd40-6, while six other genes (C3, LDLR, EF2, GPI, PEPD and MANB) were retained. Cytogenetic analysis and in situ hybridization using human or mouse repeated sequences as probes showed that the region q13.1-qter of human chromosome 19 had been replaced by a fragment of mouse chromosome. Our results permit further regional assignment for the following five genes on human chromosome 19: GPI in the region cen-q12, MANB in p13.2-q12, E11S and RDRC in q13.1-qter, and EF2 in pter-q12.

Animals↗