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Biomedical subjects

H Herrmann

Publications and source records attributed to H Herrmann.

At least 145 records · Page 8Linked to original sources
PubMed1977

[Smoking habits and their influence on the development of chronic broncho-pulmonary diseases--results of epidemiologic research on bronchitis in the German Democratic Republic].

Systematic studies were performed in 1971 and 1973 to investigate the epidemiology of chronic bronchitis. The scheme comprised random groups of a sum total of 22176 men and 10829 women aged between 35 and 64 from 25 places all over the territory of the GDR. The examination program comprised an interview with a questionnaire, measurement of FVC, FEV1.0, PO2 and RV/TK, examination of bronchial hyperreactivity. Representative statements emerged as to prevalence of smokers and ex-smokers. The mean prevalence of male smokers was 63 p. c., of femal smokers 24 p. c. The prevalence of chronic bronchitis is higher in smokers than in nonsmokers especially in the polluted area. Significant relationship was found between increasing tobacco consumption and prevalence of chronic bronchitis. Intensive smoking is associated with an increase in obstruction and in bronchial hyperreactivity. The study provides the evidence of the additive effects of smoking, air pollution and dust exposure at work.

Adult↗
PubMed1976

Conservative bladder treatment in myelodysplasia. Differentiation of patients by cystomanometry.

Urodynamic evaluation was performed in 75 patients with congenital neuropathic bladder. Concerning treatment the most important parameters were detrusor tone, detrusor activity and bladder outlet resistance. Based on these findings individual conservative therapy was applied. Reflux or dilatation of the upper urinary tract found in 26 children could be controlled by drugs or transurethral operations in 20, urinary diversion had to be done only 4 times. 26 children were selected for intensive treatment of incontinence; in about 50% satisfying urinary control was achieved. These results still need long-term observation. The main interest, however, was to postpone irreversible surgery and to gain time for possible better treatment in the future.

Adolescent↗
PubMed1976

[Lysosomal proteinasen and peptidasen in serum of children with inflammatory diseases (author's transl)].

In the serum of 43 children the activities of proteinases and peptidases by mean of 41 substrates have been determined in order to get knowledge of overall activities and differentiation of lysosomal proteolytic enzymes. Proteinases, cathepsins A, B, C and D, aminopeptidases, carboxypeptidases, dipeptidases, tripeptidases and aminoacidarylamidases have been checked. The enzyme pattern of the serum of a collective of 15 healthy children or those without serious clinical signs is demonstrated, also the alterations and differentiations in the serum of children with leucemia, pneumonia, inflammatory diseases of the respiratory tract, other inflammatory diseases and common diseases. Leucyl-glycyl-glycyltripeptidase, glycyl-glycyl-glycyltripeptidase, a proteosterase, carboxypeptidase A, a neutrale proteinase and basic proteinase (cathepsin B) and cathepsin C are increased. A distinct elevation has been found only in children with leucemia and pneumonia.

Aminopeptidases↗
PubMed1976

[Frequency of chronic bronchitis-results of epidemiologic research in the GDR (author's transl)].

The frequency of chronic bronchitis among 33000 persons, selected by randomization, between 35 and 64 years of age, living in 4 large cities and 24 selected districts with different air pollution levels in GDR was investigated. A chronic bronchitis questionnaire and measurement of FVC, FEV1, PO2 were used to test each person, partially broncho-provocation-tests with histamin aerosols were performed. According to this investigation 6% of the men and 2.8% of the women suffered from CNSLD. In this group of diseased persons 50% showed obstructive disturbances of ventilation, 20% signs of decreased physical fitness due to respiratory disorders and 10% a manifested respiratory insufficiency.

Adult↗
PubMed1975

Incorporation of fucose and glucosamine into cell bound and medium released macromolecules.

(1) Determinations were carried out on the incorporation of fucose-6-(3H) and glucosamine-6-(3H) into trichloracetic acid insoluble macromolecules which remained bound to the cells or were released into the medium of chick embryo muscle cell cultures. The radioactivity determined in the medium was corrected for unspecific binding of label to components of the medium. (2) During an incorporation period of six hours the incorporation per microgram DNA with fucose as label into cell bound macromolecules is about twice as high as the incorporation into macromolecules released into medium. With glucosamine about twice as much is incorporated into medium released into the cell bound macromolecules. (3) The incorporation per microgram DNA increased during a culture period of three days but the increase ceases at different times during this culture period when determined with fucose or glucosamine or for cell bound and medium released material. (4) An increase in cell density increases the incorporation per DNA of fucose and to a much slighter extent that of glucosamine. Reduction of cell density by addition of cytosine arabinoside to the medium does not increase the incorporation per microgram DNA. (5) The effect of changes of fibroblast/myoblast ratios on the incorporation of fucose and glucosamine were examined. No significant effect was observed for a ratio of 10-30% fibroblasts when control cultures or cultures after cell sedimentation were maintained in complete medium. Marked changes were observed after culture in medium without protein components. Under these conditions an increase in the fibroblast/myoblast ratios were observed as well as an increase in the incorporation of label into medium released and a decrease into cell bound macromolecules.

Animals↗
PubMed1975

[Vitamin B 12 dependent methionine biosynthesis in pseudomonas aeruginosa].

Among methionineless mutants of Pseudomonas aeruginosa strain PAO lacking the ability of methylating homocysteine we found two different types: One of them responding to methionine only, the other one to methionine or vitamin B12 alternatively. That means that P. aeruginosa PAO as well as other B12-producing bacteria (with one exception: Aerobacter aerogenes) use only the B12 pathway (metH) for methionine synthesis. The effect of some cys-auxotrophs equally growing on vitamin B12 as described by Calhoun and Feary (1969) was confirmed for P. aeruginosa PAO by our mutants. In a P. aeruginosa strain of other origin, PAE, neither met- nor cys-mutants responding to B12 have been found, although strain PAE as well as strain PAO excessively synthesize B12.

Cysteine↗
PubMed1975

Immunological and chemical purity of papain-solubilized HL-A antigens.

Three preparations of purified papain-solublized HL-A antigens have been radiolabeled by reductive methylation using formaldehyde and potassium boro[3H]hydride, and their reaction with specific HL-A antisera has been investigated. Greater than 99 percent of the radioactivity in the [3H]HL-A2 preparation could be complexed with several HL-A2 antisera, but not with specificity controls. The other two preparations, which contained mixtures of HL-A antigenic specificities (HL-A7,12 an HL-A3,W25;12,27), showed 63 per cent and 70 per cent complex formation with mixtures of the appropriate HL-A antisera. The N-terminal amino acid of both subunits has been determined for the three HL-A antigen preparations. In all cases the only detectable N-terminal amino acids were isoleucine for the small subunits, beta-2-microblogulin, and glycine for the larger subunit.

Amino Acid Sequence↗
PubMed1975

Isolation and characterization of type III collagen from chick stain.

A collagen species was isolated from the skin of 8 week-old chicks by pepsin treatment at 4 degrees C and was identified as type III collagen by analogy to human and calf type III collagen. Like human type III collagen, the type III collagen molecule from chick skin is composed of three alpha1 (III) chains which are linked together by disulfide bridges. The amino acid composition of [alpha1 (III)]3 purified by CM-cellulose chromatography and agarose-gel chromatography includes two cysteine residues per chain, and the hydroxyproline/proline ratio is greater than 1.0. The cyanogen bromide peptide pattern of [alpha1 (III)]3 on sodium dodecylsulfate acrylamide gel electrophoresis differed from that of alpha1 (I), alpha1 (II) and alpha2 chains. Segment-long-spacing crystallites from chick skin type III collagen showed the same cross striation pattern as segment-long-spacing crystallites from calf skin type III collagen. Unlike human type III collagen, type III collagen from chick skin was highly soluble in 1.0M NaCl, 0.05M Tris/HCl buffer, pH 7.5, and could not be separated from type I collagen by fractional salt precipitation. On CM-cellulose chromatography, [alpha1 (III)]3 molecules from chick skin eluted in the same position as alpha2 chains, in contrast to human [alpha1 (III)]3, which elutes in the position of beta12.

Amino Acids↗