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Biomedical subjects

H Hess

Publications and source records attributed to H Hess.

At least 19 recordsLinked to original sources
PubMed1992

High-level secretion of the four salivary plasminogen activators from the vampire bat Desmodus rotundus by stably transfected baby hamster kidney cells.

The cDNAs coding for the four Desmodus rotundus salivary plasminogen activators (DSPAs) were subcloned into the mammalian expression vector, pMPSV/CMV, which carries the myeloproliferative sarcoma virus promoter and the cytomegalovirus enhancer. These constructs were transfected, together with plasmids harbouring Geneticin (G418)-resistance and puromycin-resistance genes, into baby hamster kidney cells. Through the selective pressure of both antibiotics, cell clones constitutively overexpressing the DSPA alpha 1, DSPA alpha 2, DSPA beta or DSPA gamma cDNAs were obtained. Secretion of active DSPAs was confirmed by zymographic analysis and quantified using a fibrin plate assay and ELISA.

Animals↗
PubMed1992

Purification and properties of a secreted and developmentally regulated alpha-L-fucosidase from Dictyostelium discoideum.

During its development the eukaryotic microorganisms Dictyostelium discoideum secretes an alpha-L-fucosidase (EC 3.2.1.51). In cells of the growth phase almost no alpha-L-fucosidase activity is detectable. The activity increases steadily up to the aggregation stage and accumulates also in the extracellular medium. The developmental regulation is mediated by pulsatile cAMP signals. The alpha-L-fucosidase was purified from extracellular medium. The isolation procedure started with concentration of the enzyme by batchwise anion-exchange chromatography and ammonium sulfate precipitation, followed by Sephacryl S-300 gel filtration and further purification by fast protein liquid chromatography on Mono Q, phenyl-Superose, and finally Superose 12. The purified preparation was found to be essentially free of activities of six other glycosidases also secreted by D. discoideum. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified enzyme showed one major band with an apparent molecular mass of 62 kilodalton. Gel filtration of the enzyme on a Superose 12 column was consistent with an active monomer. A monoclonal antibody was produced, which recognizes a carbohydrate epitope shared by all lysosomal enzymes in D. discoideum. The pH optimum of the alpha-L-fucosidase is at 3.7. The apparent Michaelis constant for p-nitrophenyl alpha-L-fucoside as substrate is 1.2 mM. The enzyme catalyzes preferentially the hydrolysis of alpha 1----6GlcNAc but also of alpha 1----2Gal and alpha 1----3Glc fucosyl linkages.

Animals↗
PubMed1992

Purification of serum amyloid A and its isoforms from human plasma by hydrophobic interaction chromatography and preparative isoelectric focusing.

The present work was aimed at isolating human serum amyloid A, (SAA), an acute-phase protein mainly complexed to high density lipoproteins, directly from human plasma without sequential ultracentrifugation of lipoproteins and subsequent delipidation of the apolipoprotein moiety. Hydrophobic-interaction fast-protein liquid chromatography on Octylsepharose, using stepwise gradient elution profiles under dissociating conditions, followed by fast-protein liquid-gel permeation chromatography on a Superdex TM75 column revealed a higher than 95% purity of isolated SAA. Further purification of SAA from coeluting apolipoproteins C and A-II was achieved by preparative isoelectric focusing between pH5-7 using a Rotofor apparatus. Separation of the main SAA isoforms, SAA1 (pI 6.5) and SAA1 des-Arg (pI 6.0, lacking the N-terminal arginine), was achieved by anion-exchange fast-protein liquid chromatography on a Fractogel EMD DEAE 650-S column. The purity of the SAA1 and SAA1 des-Arg isoforms, thus isolated, was checked by immunochemical techniques and amino acid analysis. With the described method various SAA isoforms can be isolated, purified and separated directly from human plasma/serum without prior ultracentrifugation.

Blotting, Western↗
PubMed1992

[A rare variation in the zone of the carotid artery].

An extremely rare variation in the angioarchitecture of the carotid arteries consists in the complete missing of the right common carotid artery. The brachiocephalic trunc (innominate artery), forming a bulbus-like extension, branches out into three major stems: internal carotid artery, external carotid artery and the right subclavian artery. The variation is embryologically explainable: The aortic arches III and IV arise together (without ventral aortic root between them) from the persisting initial segment of the right ventral aorta.

Aorta, Thoracic↗
PubMed1991

[The Malleotrain bandage in a large clinical trial].

The authors tested the clinical efficacy of the malleotrain bandage in a clinical field trial on a very large mixed patient material from 1980 onwards. The patient material included a total of 350 cases of ankle joint injuries with partial and total fibular ruptures of ligament, ruptures of the inferior tibiofibular joint, conditions after malleolar fractures as well as posttraumatic or postoperative swelling.--It was found that the malleotrain bandage achieved subsidence of swelling of the periarticular soft parts, relief of complaints and considerable normalisation of functioning, within an unusually short time and without medication or other local measures.--Patients who had been operated on for complete rupture of fibulotalar ligament were given by Lippay the malleotrain bandage only, after having immobilised the malleolus by means of a plaster cast for 10 days, without any disadvantages in respect of healing and subsequent stability.--Blandfort had even treated complete lateral ankle joint ligament ruptures conservatively with the malleotrain bandage only and had thus achieved cure resulting in stable ligaments.--The ability to work and to resume sports activities returned on the average two weeks earlier than with plaster cast immobilisation.

Adolescent↗
PubMed1991

[Kimura disease--2 case reports].

Two histologically confirmed cases of Kimura's disease are reported in the present article. Emphasis is placed on the clinical symptoms in conjunction with the histological picture. Recent studies in the literature are discussed with respect to differential diagnosis, etiology and therapy. In all ENT-tumours of unknown origin this relatively uncommon disease should also be considered.

Angiolymphoid Hyperplasia with Eosinophilia↗
PubMed1991

[Local low dosage thrombolysis with rt-PA. Systemic effects on the fibrinolytic system--data on the assessment of the value and risk of this method].

We investigated 70 patients suffering from peripheral vascular disease for alterations in fibrinolytic parameters before, during and twice after local, low dose thrombolysis with rt-PA (1 - 7.5 mg). Recanalization was achieved in all 10 embolic occlusions and in 87% of 60 thrombotic occlusions. We found no alterations of fibrinogen and plasminogen plasma levels, but significant consumption of antiplasmin 5 min after the end of rt-PA application. Degradation products of fibrinogen and fibrin showed a parallel increase only during the therapy. PAI I concentrations significantly increased until the 2nd day after the therapy. Since we limited the maximum dose of rt-PA to 7.5 mg we never saw systemic bleeding or embolisation in more than 300 cases.

Aged↗
PubMed1990

[Experiences with joint-preserving operations in arthrosis of the upper ankle joint].

During a period of ten years we performed 140 joint conserving operations on patients with arthrosis of the ankle joint. The use of this procedure was analysed in 85 patients, whom we followed up. Mainly (in 89%) positive results were obtained. The success of such joint conserving surgery depends on the one hand on the seriousness of the injury leading to arthrosis and on the other hand on the duration of the complaints. If attention is paid to these factors we have after the failure of conservative therapy a satisfying complementary treatment before using the arthrodesis.

Adolescent↗
PubMed1990

[Digital subtraction angiography using carbon dioxide: an alternative to arteriography of the extremities using iodine-containing contrast media].

The basics and the technique of intraarterial digital subtraction arteriography with carbon dioxide (CO2) are presented. The procedure turned out to be a good and relatively poor-risk alternative to conventional angiography with iodinated contrast media in 26 peripheral arteriographies and in 6 peripheral angioplasties in hyperthyroid patients. Using a 9-inch amplifier, arteriograms from the infrarenal abdominal aorta down to the lower leg arteries were performed with five series of insufflation of 20-25 cc CO2 each. With regard to the image quality, there is no difference between CO2 arteriograms and those performed with intraarterial application of iodinated contrast media.

Aged↗
PubMed1989

[Local thrombolysis in peripheral arterial occlusion].

Peripheral arterial occlusions, with the exception of those induced mechanically or by vasospasm, are invariably caused by a blood clot resulting from either in-situ thrombosis or embolism. More than 10% of embolic occlusions in otherwise healthy arteries undergo spontaneous lysis due to the organisms tissue plasminogen activator. In thrombotic occlusion of arteriosclerotic vessels, probably due to insufficient activator release from the diseased arterial wall, spontaneous lysis is much less common. For more than 25 years, lysis has been aided with streptokinase (SK) or urokinase (UK) which, until eight years ago, had only been given systemically with a standard dosage of 2.4 million units daily for up to five days. Thrombotic femoral artery occlusions of up to six weeks old were successfully lysed in 48%, six to twelve weeks old in 25% and, in those older than twelve weeks only in exceptional cases. With embolic occlusion, systemic lysis is contraindicated due to the possibility of provoking new emboli. With conventional systemic SK treatment, in 7% of the patients there was severe bleeding which in 1.12% was fatal. The ultrahigh SK treatment (nine million units in six hours) has substantially fewer bleeding complications but no better rate of success. Systemic administration of SK and UK leads to activation of the entire circulating plasminogen and the correspondingly-associated clotting defects. Recombinant tissue plasminogen activator (rt-PA), the production of which was rendered possible by genetic engineering, is identical to human tissue activator, has a high affinity to fibrin-bound plasminogen, less affinity to circulating plasminogen. After systemic administration, however, the plasminogen in every vascular clot is activated such that, even without alteration of the clotting system, bleeding the emboli can be provoked. With local application of the activator, even extensive clots, provided they contain lysable fibrin, can be dissolved within one-half to three hours with comparably minimal doses. For local lysis treatment of peripheral arterial occlusions, SK, UK and rt-PA are well-suited. With a total dose of maximally 30,000 units SK, in contrast to the initially-used higher doses, there were no bleeding complications in more than 300 patients. Even with a total doses of 100,000 to 300,000 UK, albeit in a relatively small number of patients, and a total dose of 2.5 to 7.5 mg rt-PA which was given within three hours maximally to 85 patients, there were no bleeding complications.(ABSTRACT TRUNCATED AT 400 WORDS)

Angioplasty, Balloon↗
PubMed1988

Femoro-popliteal artery thrombolysis with intra-arterial infusion of recombinant tissue-type plasminogen activator--report of a pilot trial.

Recombinant tissue-type plasminogen activator (rt-PA) was infused at a rate of 10 mg/h into 50 thrombosed femoral and popliteal arteries. Patency was restored in 43 but a secondary angioplasty led to 2 reocclusions and in 3 patients early rethrombosis occurred. A favourable clinical result was thus obtained in 38 patients (76%). Thirteen bleeding complications occurred in 10 patients, mainly haematomas at puncture sites. One patient required blood transfusion for gastro-intestinal bleeding from a previously unknown ulcer. The angiographic recanalisation rate in 16 patients who received a slower infusion of rt-PA (5 or 3 mg/h) was 94% and the clinical success rate in this series was 81%. However, the incidence of bleeding complications was not decreased by the slower infusion rate. The data obtained confirm the feasibility of rt-PA thrombolysis in peripheral arterial thrombosis and warrant a comparative study with streptokinase.

Angioplasty, Balloon↗
PubMed1988

[Fibrinolysis with rt-PA in peripheral arterial occlusions].

First experiences using rt-PA for the local lysis of peripheral arterial occlusions have shown that it is a potent activator of the fibrinolytic system. 2 to 5 mg of rt-PA administered for 1 to 1 1/2 hours are sufficient to completely dissolve even long occlusions. With doses up to 20 mg over 2 hours no systemic bleeding was observed. With 50 mg given within 5 hours and with an infusion of 2.5 mg per hour for 48 hours there were two cases of systemic haemorrhaging entirely due to the fibrinolysis. No appreciable defects in the coagulation system and no other side effects were observed.

Aged↗
PubMed1987

[Local thrombolysis in the treatment of acute ischemia of the leg].

The results of local low-dose thrombolytic treatments of 564 peripheral arterial occlusions were as follows: Embolic occlusions up to 6 weeks could be removed in 72.7%, the longer lasting occlusions in 42.3%. Thrombotic occlusions lasting up to 6 months were successfully treated in 58%, still older ones in 36.2%. The cumulative patency after 5 years was 89.5% in embolic and 58.8% in thrombotic occlusions. Therefore, the differential therapy of acute peripheral ischemia has to be thought over anew together with the vascular surgeons.

Acute Disease↗
PubMed1987

Peripheral arterial occlusions: a 6-year experience with local low-dose thrombolytic therapy.

Early and long-term results of treatment with local low-dose thrombolysis in 554 patients with 564 peripheral arterial occlusions are reported. Of 92 embolic occlusions present for 2 months or more, 59 (64.1%) were recanalized with a cumulative patency of 89.5% after 5 years. Of 472 thrombotic occlusions present for up to 6 months and more, 254 (53.8%) were successfully treated with a cumulative patency of 58.8% after 5 years. The hospital mortality and amputation rate were 1.6% and 1.95%, respectively. The average age of the patients was 69.1 years and more than half of those treated had stage III or IV disease. A 6-year experience with local low-dose thrombolytic therapy has completely confirmed its efficacy and has led to improvements in technique, which are described. The doses of streptokinase and urokinase needed for a successful result have been substantially reduced and the duration of treatment shortened. The number of complications has also been reduced. Differential therapeutic considerations compared to vascular surgery are mentioned. The results should motivate a reconsideration of the diagnostic and therapeutic measures to be used in the treatment of peripheral arterial occlusions.

Adolescent↗