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Biomedical subjects

H Holtzer

Publications and source records attributed to H Holtzer.

At least 127 records · Page 7Linked to original sources
PubMed1972

Cytochalasin B: effects on cell morphology, cell adhesion, and mucopolysaccharide synthesis (cultured cells-contractile microfilaments-glycoproteins-embryonic cells-sorting-out).

Cytochalasin B reversibly causes extensive branching of myoblasts, fibroblasts, and nonencapsulated chondroblasts; it does not induce the formation of similar processes in myotubes, erythrocytes, amnion cells, encapsulated chondroblasts, or HeLa cells. The drug has no effect on the spontaneous contractions of isolated skeletal, cardiac, or smooth-muscle cells. Within 60 min, it depresses the incorporation of [(14)C]glucosamine into total mucopolysaccharide and glycoproteins by over 50%. The drug interferes with adhesion and sorting-out of dissociated embryonic cells. Cytochalasin B is likely to produce changes in components of the cell surface whose function is not readily or solely related to a system of "primitive contractile microfilaments."

Amnion↗
PubMed1972

Some properties of embryonic myosin.

Myosins from the following sources were purified by diethylaminoethyl-Sephadex chromatography: moytubes grown in vitro for 7-8 days, prepared from pectoralis muscles of 10-day old embryos, and breast and leg muscles from 16-day old embryos. The adenosine triphosphatase activities of these myosins were close to that of adult m. pectoralis myosin. The light chains of the embryonic myosins had the same mobilities in sodium dodecyl sulfate electrophoresis as those in adult pectoralis muscle myosin and were clearly distinguishable from those in myosin from tonic muscle m. latissimus dorsi anterior. The fastest light chain in embryonic muscle myosin-apparent mol wt 16,000-was present in smaller amounts than in adult myosin. The negative staining pattern of paracrystals of embryonic light meromyosin (LMM) was indistinguishable from that of adult fast muscle LMM. The significance of these results for differentiation of various muscle types has been discussed.

Adenosine Triphosphatases↗
PubMed1971

Primitive erythropoiesis in early chick embryogenesis. I. Cell cycle kinetics and the control of cell division.

The primitive line of embryonic chick blood cells develop as a relatively homogeneous cohort of cells. Using an analysis based on the continuous uptake of thymidine-(3)H, we have established the generation time, G1, S, and G2 for progressively more mature generations of these immature erythroblasts. The data indicate that after the initiation of hemoglobin synthesis, the average cell will yield six generations of hemoglobin producing erythroblasts. The older generations of erythroblasts exhibit a longer generation time, G1, S, and G2 than the earlier generations of erythroblasts. Other methods of analysis corroborated these findings. One of these methods, an estimate of total erythrocyte productivity from the primitive stem cells (hematocytoblasts), led to the conclusion that the erythroblast cell lineage might be initiated as early as the sixth or seventh division following fertilization. In addition, primitive erythroblasts characterized by one set of cell cycle parameters, when grown in serum associated with erythroblasts of different parameters, showed no alteration in mitotic behavior. These results suggest the presence of programmed cell division not immediately cued by extracellular influence.

Age Factors↗
PubMed1971

Primitive erythropoiesis in early chick embryogenesis. II. Correlation between hemoglobin synthesis and the mitotic history.

Primitive erythroblasts in the circulating blood of the chick embryo continue to divide while synthesizing hemoglobin (Hb). Hb measurements on successive generations of erythroblasts show that there is a progressive increase in the Hb content of both interphase and metaphase cells. Furthermore, for any given embryo the Hb content of metaphase cells is always significantly greater than that of interphase cells. The distribution of Hb values for metaphase cells suggests that there are six Hb classes corresponding to the number of cell cycles in the proliferative phase. The location of erythroblasts in the cell cycle was determined by combining Feulgen cytophotometry with thymidine radioautography on the same cells. Measurements of the Hb content for erythroblasts in different compartments of the cell cycle (G1, S, G2, and M) show a progressive increase through the cycle. Thus, the amount of Hb per cell is a function of the number of cell divisions since the initiation of Hb synthesis and, to a lesser degree, the stage of the cell cycle. Earlier generations of erythroblasts synthesize Hb at a faster rate than the terminal generation. Several models have been proposed to explain these findings.

Animals↗
PubMed1970

Inhibition of myoblast fusion after one round of DNA synthesis in 5-bromodeoxyuridine.

The thymidine analogue 5-bromodeoxyuridine (BUdR) has a differential effect on the synthesis of tissue-specific products and molecules required for growth and division. Proliferating myogenic cells cultured in BUdR fail to fuse and fail to initiate the synthesis of contractile protein filaments. Conversely, BUdR has but a minor effect on cell viability and reproductive integrity. Low concentrations of BUdR result in an enhancement of cell number relative to the controls; higher concentrations are cytotoxic. Suppression of myogenesis is reversible after at least 10 cell generations of growth in the analogue. Cells that do not synthesize DNA, such as postmitotic myoblasts and myotubes, are not affected by BUdR. Incorporation of BUdR for one round of DNA synthesis was accomplished by first incubating myogenic cells, prior to fusion, in 5-fluorodeoxyuridine (FUdR) to block DNA synthesis and collect cells in the presynthetic phase. The cells were then allowed to synthesize either normal DNA or BU-DNA for one S period by circumventing the FUdR block with BUdR or BUdR plus thymidine (TdR). The cultures were continued in FUdR to prevent dilution of the incorporated analogue by further division. After 3 days, the cultures from the FUdR-BUdR series showed the typical BUdR effect; the cells were excessively flattened and few multinucleated myotubes formed. Cells in the control cultures were of normal morphology, and multinucleated myotubes were present. These results were confirmed in another experiment in which BUdR-(3)H was added to 2-day cultures in which myotubes were forming. Fusion of thymidine-(3)H-labeled cells begins at 8 hr after the preceding S phase. In contrast, cells which incorporate BUdR-(3)H for one S period do not fuse with normal myotubes.

Animals↗