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Biomedical subjects

H Horiguchi

Publications and source records attributed to H Horiguchi.

At least 91 records · Page 5Linked to original sources
PubMed1989

Lung cancer-associated monoclonal antibody 15 that recognizes cell growth-related membrane antigens gp85/45 and its growth-inhibitory effect on human lung cancer cells in vitro.

In order to find lung cancer-specific markers, monoclonal antibody 15 (MAb15) was produced against a variant-type cell of small cell lung carcinoma. Its gp85/45 antigens were demonstrated in 70% of lung cancers, and particularly in the proliferating zone of cancer cell nests, but they are scarcely detected in noncancerous tissues. Immunoelectron microscopy revealed that gp85/45 antigens were expressed alternatively on the cell membrane of living cancer cells according to their biological states. MAb15 added to the culture medium inhibited the proliferation of lung cancer cells, depending on its concentration, but cell death rate did not increase. The growth inhibition by MAb15 was reevaluated by a colonogenic assay. On DNA histogram, MAb15 decreased the number of DNA-synthesizing cells in the S phase with an elevation of the G1 peak, indicating a G1-S boundary block in the cell cycle. gp85/45 detected by this lung cancer-associated monoclonal antibody could be a functional membrane unit, such as a growth factor receptor, which is related to the cell proliferation of lung cancer. The growth inhibition by MAb15 may be caused by the blocking of a growth factor receptor which is specific to lung cancer.

Antibodies, Monoclonal↗
PubMed1989

[Research on aseptic condition of root canals after endodontic treatment].

Fifty infected root canals were examined whether they had been in aseptic condition or not after endodontic treatment. PLADIA was used for the examination of bacteriologic culture. Twenty eight cases were positive, 22 cases were negative, and the percentage of positive was 56%. Of the anterior teeth, 13 cases were positive in 23 cases (57%), In the posterior teeth, 15 cases were positive in 27 cases (56%), 5 cases were positive in 17 cases (29%) with clinical symptoms before treatment, 23 cases were positive in 33 cases (70%) without clinical symptoms before treatment. These results showed that the treated root canals which had been well adapted to root canal filling by clinical symptoms had a high percentage of positive of bacteriological culture.

Bacteria↗
PubMed1989

[Measuring of thresholds of the periodontal diseased teeth by electric pulp tester].

Studying of influence of periodontal disease on vitality of pulp, the response thresholds of 421 teeth (250 normal teeth, 171 periodontal diseased teeth) were examined electronically by means of an automatic pulptester. (ANALYTIC TECHNOLOGY CO.) The pulp tester values of three experiments were averaged. The average value of normal teeth was 36.3 and that of periodontal diseased teeth 31.4. The response thresholds of periodontal diseased teeth were lower than those of normal teeth. This indicated that pulp was inflamed by periodontal disease and pulp vitality became weak.

Dental Pulp Diseases↗
PubMed1989

[Investigated reports of tooth fractures cases from November, 1986 to September, 1988].

Researching the actual condition of tooth fractures, clinical records and X-ray photographs of 2717 patients, visited P.D.I. Dental Clinic from November, 1986 to September, 1988 were investigated statistically. 174 cases were tooth fractures in 2717 patients. The frequency rate of tooth fractures at an early age was low, increased from the latter half of the 30s and was highest at the latter half of the 50s accounted for over 10%, and decreased from the 60s. In the upper anterior teeth, the crown fractures caused by traumatic factors, at an early age showed a high proportion, and in the lower anterior the tooth fractures were rare. In the posterior the root fractures of non-vital teeth attributed to dental treatment at the middle and advanced age indicated a high proportion. In the case of tooth restored with metalcore and bridge, the proportion of the root fractures were high, the metalcore, was used in 63%, and in the bridge in 27% of the root fractures.

Adult↗
PubMed1989

[Influence of surface properties on the bending strength of OPTEC porcelain].

Influence of properties on bending strength of OPTEC porcelain, an experiment on four-point bending test was studied using four kinds of specimens (5 X 3 X 20) made of OPTEC porcelain (INCISAL) each of which was surface-treated; (1) glaze firing (no treatment) (2) polishing by emery paper #150 (3) polishing by alumina powder (0.3 microns) (4) glaze firing after polishing by emery paper #150 The test specimens were immersed in distilled water maintained at 37 +/- 1 degrees C broken into four-point bending at 1.0 mm/min, and their bending jig had on inner and outer span of 5 mm and 15 mm. The specimens graze fired after polishing had the highest bending strength, of 914 kg/km2, those polished with alumina powder, 831 kg/cm2, those graze 763 kg/cm2, and those polished with emery paper 705 kg/cm2. In conclusion, the bending strength of the porcelain was decreased by the surface roughness.

Dental Porcelain↗
PubMed1988

Chemotaxis for methamphetamine in Escherichia coli.

Escherichia coli showed the chemotactic response for methamphetamine, a biogenic stimulative amine. The response was qualitatively detected by using a paper disk filter. The quantitative assay showed that E. coli responded to methamphetamine at 10(-5) M or more.

Chemotaxis↗
PubMedDate not supplied

Meningioma with granulofilamentous inclusions.

The authors report a case of intracranial meningioma with granulofilamentous inclusions. A 50-year-old man had right trigeminal neuralgia due to trigeminal nerve compression by a petroclival tumor and received tumor resection. Microscopically, tumor cells containing eccentric nuclei and intracytoplasmic hyaline inclusions were arranged in sheets and whorls. The inclusions were negative for periodic acid-Schiff reaction. No histological anaplasia was seen. Immunohistochemistry showed epithelial membrane antigen reactivity on the cytoplasmic membrane. Immunoreactivity for vimentin was recognized in cytoplasm adjacent to inclusions. However, confocal laser microscopic study revealed immunoreactivity for vimentin even inside some inclusions. Ultrastructurally, interdigitation of cytoplasmic processes and desmosomes connecting adjacent cells were noted. Inclusions were composed of numerous fine osmiophilic granules attached by intermediates filaments. These findings were consistent with a meningioma with the granulofilamentous inclusions described earlier. The findings demonstrated by confocal laser microscopy and electron microscopy suggest that these granular materials may be the metabolic products of vimentin filaments.

Humans↗