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Biomedical subjects

H Hosotsubo

Publications and source records attributed to H Hosotsubo.

26 records · Page 2Linked to original sources
PubMed1989

Rapid screening for Staphylococcus aureus infection by measuring enterotoxin B.

We developed a simple, sensitive laser nephelometric assay (LNA) to detect circulating staphylococcal enterotoxin B (SEB). This assay yields the result within 2 h and needs no special treatment. Influence of protein A, a product generated by Staphylococcus aureus, was negligible in this assay. The levels of SEB in plasma were measured in 28 patients with and without S. aureus infection with the LNA. The levels of SEB in plasma increased significantly in patients with S. aureus infection. We also demonstrated that the levels of SEB in plasma were higher in patients with methicillin-resistant S. aureus infection than in patients with nonresistant staphylococcal infection. Our data indicate that LNA is a useful assay in the early diagnosis of methicillin-resistant S. aureus infection.

Cross Reactions↗
PubMed1988

Rapid determination of amphotericin B levels in serum by high-performance liquid chromatography without interference by bilirubin.

A high-performance liquid chromatographic method for the determination of amphotericin B concentrations in human serum without bilirubin interference was developed and compared with a microbiological assay. The high-performance liquid chromatographic assay utilized a reversed-phase trimethyl silica column, simple sample preparation, and visible detection. Reproducibility studies yielded coefficient-of-variation ranges from 1.02 to 2.11% for within-day precision and 2.88 to 4.32% for between-day precision. The correlation coefficient with the microbiological assay was 0.984 for amphotericin B.

Amphotericin B↗
PubMed1987

Immunofluorescence determination of IgG in cerebrospinal fluid following high performance liquid gel permeation chromatography.

A simple technique using high performance liquid gel permeation chromatography for determination of IgG in cerebrospinal fluid (CSF) is described. Accurate determination of IgG was achieved by precolumn derivatization with fluorescein isothiocyanate-conjugated anti-human IgG Fab, a substance that readily yields a fluorescent immune complex. Immune complexes separated as a well-delineated peak in the column void volume, and were measured by the fluorescence of the column eluates (Ex = 490 nm, Em = 520 nm). The results were reproducible with a deviation of less than 2% and a good linear relationship over the range of 2.0-24.5 mg/dl. The IgG concentration assayed by the column method correlated well with values obtained by laser nephelometry.

Antibodies↗
PubMed1986

Determination of cyclosporin A in whole blood by high-performance liquid chromatography using automated column switching.

A fully automated high-performance liquid chromatographic column-switching system is presented for the determination of cyclosporin A in whole blood. After blood proteins were precipitated with acetonitrile, the supernatant was automatically loaded on to a cyanopropyl column for initial separation, and then the fraction containing cyclosporin A was loaded on to a trimethylsilica column for final separation and quantitation. Cyclosporin A was detected by ultraviolet absorption at 205 nm. The minimum detectable concentration of cyclosporin A was 5 ng/ml in 100 microliter of blood. The coefficient of variation of the method was 1.755, 1.748 and 0.655% in whole blood when spiked at the 170, 425 and 850 ng/ml levels, respectively. One assay was completed in 15 min.

Autoanalysis↗
PubMed1985

Immunofluorescence determination of IgG following high performance liquid gel permeation chromatography.

The application of high performance liquid gel permeation chromatography (HPL-GPC) and immunofluorescence for the estimation of a protein was studied using human IgG as a model system following separation of the fluorescent immune complex from free fluorescent-labelled antibody on a column of HPL-GPC. Using FITC-labelled anti-human IgG Fab fragment and HPL-GPC, nanogram levels of IgG could be determined within 5 min of mixing the antigen and antiserum. HPL-GPC immunofluorescence analysis is sensitive specific, simple, rapid and highly versatile.

Antigen-Antibody Complex↗