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Biomedical subjects

H Hou

Publications and source records attributed to H Hou.

At least 37 records · Page 2Linked to original sources
PubMed1999

[The relationship between operation on gastrointestinal cancer and single cells metastasis via portal vein].

OBJECTIVE: To confirm the effect of radical operation on single cells metastasis of gastrointestinal cancer. METHODS: One hundred and seventy two patients with gastrointestinal cancer were divided randomly into group I (111 cases, ordinary operation) and group II (61 cases, operation after ligation of drained vessels). Catheters were inserted into the portal vein during operation. Blood was aspirated and contrifugated. Anti-EMA monoclonal antibody marked the cancer cells, which were shown by ABC immunohistochemical staining. RESULTS: In the examinate group, 72 cases of gastrointestinal cancer were found positive cells among 111 cases (positive rate 64.9%). In the control group, 61 cases were tested and 13 cases were found positive cells (positive rate 21.3%) with a highly significant difference between the two groups (P < 0.005). CONCLUSIONS: Ligating the relative blood vessel before the radical operation can prevent single cancer cells from metastasis via portal vein.

Adult↗
PubMed1999

[Phase variation in Salmonella typhimurium phagetype].

The phase variation of phagetype was observed in cultures of Salmonella typhimurium collected from Xinjiang Uygur Autonomous Region during 1980-1993. A few cultures of phagetype 4774 or 4776(full type) may change into another phagetype, i.e. 4000(phase 1) or 0774 and 0776 (phase 2). In reverse, more strains of these phagetype 4000 and 0774(0776) easily back into their original phagetype 4774 or 4776. But some 4000(phase 1) can change to 0776(phase 2), and some 0774(phase 2) can also change to 4000(phase 1). Similarly, a few cultures of phagetype 7776(full type) can change to 7000(phase 1) or 0776(phase 2). More strains of these phagetype 7000(or 7002) and 0776 reverse easily to their original phagetype 7776 or 7774. The variability is 15.6% from full type to phase 1 or phase 2, and is 53.2% from phase 1 or phase 2 to full type. Understanding of phase variation of Salmonella typhimurium phagetype will be helpful to Salmonella phagetyping, and will be significance on epidemiological analysis of Salmonella infection.

Bacteriophage Typing↗
PubMed1999

[A Raman spectral studies of rare-earth (REE) fluoro-carbonate minerals].

The Raman spectra of REE fluoro-carbonate minerals were studies by a type of RIT-30 laser Raman spectrometer. On the basis of Raman spectral properties, REE fluoro-carbonate minerals can be divided into three groups: Ba-REE fluoro-carbonate mineral, Ca-REE fluoro-carbonate mineral and REE fluoro-carbonate mineral. Obviously, a differential kind of mineral is different in the vibration frequency of the Raman spectra. They are as follows: Cordylite v1-1 088, v2-967, v3-1 538, v4-720, 628; Hauheite v1-1 089,v3-1 525, 1 596, v4-720, 649; Cerbaite v1-1 088, v2-911, v3-1 516, v4-718,625; Parisite v1-1 095,1 075, v2-915, v3-1 461, v4-744, 732; Bastnesite v1-1 098, v2-835, v3-1 476, 1 447, v4-732.

English Abstract↗
PubMed1998

Role of Mxi1 in ageing organ systems and the regulation of normal and neoplastic growth.

Mxi1 belongs to the Mad (Mxi1) family of proteins, which function as potent antagonists of Myc oncoproteins. This antagonism relates partly to their ability to compete with Myc for the protein Max and for consensus DNA binding sites and to recruit transcriptional co-repressors. Mad(Mxi1) proteins have been suggested to be essential in cellular growth control and/or in the induction and maintenance of the differentiated state. Consistent with these roles, mxi1 may be the tumour-suppressor gene that resides at region 24-26 of the long arm of chromosome 10. This region is a cancer hotspot, and mutations here may be involved in several cancers, including prostate adenocarcinoma. Here we show that mice lacking Mxi1 exhibit progressive, multisystem abnormalities. These mice also show increased susceptibility to tumorigenesis either following carcinogen treatment or when also deficient in Ink4a. This cancer-prone phenotype may correlate with the enhanced ability of several mxi1-deficient cell types, including prostatic epithelium, to proliferate. Our results show that Mxi1 is involved in the homeostasis of differentiated organ systems, acts as a tumour suppressor in vivo, and engages the Myc network in a functionally relevant manner.

Aging↗
PubMed1998

Somatic inactivation of Pkd2 results in polycystic kidney disease.

Germline mutations in PKD2 cause autosomal dominant polycystic kidney disease. We have introduced a mutant exon 1 in tandem with the wild-type exon 1 at the mouse Pkd2 locus. This is an unstable allele that undergoes somatic inactivation by intragenic homologous recombination to produce a true null allele. Mice heterozygous and homozygous for this mutation, as well as Pkd+/- mice, develop polycystic kidney and liver lesions that are indistinguishable from the human phenotype. In all cases, renal cysts arise from renal tubular cells that lose the capacity to produce Pkd2 protein. Somatic loss of Pkd2 expression is both necessary and sufficient for renal cyst formation in ADPKD, suggesting that PKD2 occurs by a cellular recessive mechanism.

Alleles↗
PubMed1997

Altered cell differentiation and proliferation in mice lacking p57KIP2 indicates a role in Beckwith-Wiedemann syndrome.

Mice lacking the imprinted Cdk inhibitor p57(KIP2) have altered cell proliferation and differentiation, leading to abdominal muscle defects; cleft palate; endochondral bone ossification defects with incomplete differentiation of hypertrophic chondrocytes; renal medullary dysplasia; adrenal cortical hyperplasia and cytomegaly; and lens cell hyperproliferation and apoptosis. Many of these phenotypes are also seen in patients with Beckwith-Wiedemann syndrome, a pleiotropic hereditary disorder characterized by overgrowth and predisposition to cancer, suggesting that loss of p57(KIP2) expression may play a role in the condition.

Abdomen↗
PubMed1997

Role for N-CoR and histone deacetylase in Sin3-mediated transcriptional repression.

Normal mammalian growth and development are highly dependent on the regulation of the expression and activity of the Myc family of transcription factors. Mxi1-mediated inhibition of Myc activities requires interaction with mammalian Sin3A or Sin3B proteins, which have been purported to act as scaffolds for additional co-repressor factors. The identification of two such Sin3-associated factors, the nuclear receptor co-repressor (N-CoR) and histone deacetylase (HD1), provides a basis for Mxi1/Sin3-induced transcriptional repression and tumour suppression.

3T3 Cells↗
PubMed1997

[Determination of caffeine and sodium benzoate contents in caffeine and sodium benzoate injection by P-matrix method].

The contents of caffeine and sodium benzoate injection may be determined by P-matrix method without separation of its components. BSAIC language is used for programming. The average recovery of caffeine is 100.0% and that of sodium benzoate is 99.95%. The variation coefficient of caffeine is 0.40% and that of sodium benzoate is 0.65%. On the basis of recovery rate and data observed it is concluded that this method is accurate, reliable, simple and rapid as compared with the standard pharmacopoeia regulations.

Caffeine↗
PubMed1996

Pasteurization of eggs in the shell.

A small percentage of all eggs may be contaminated with Salmonella enteritidis (SE). To eliminate this hazard from the food supply, procedures for pasteurizing eggs in the shell have been developed. At least four research groups are attempting to devise a process to achieve a pasteurized shell egg. Only one of the groups has reported procedures and results. Sound shell eggs were washed to remove surface contaminants. The clean eggs were then inoculated with high levels of SE cells. The inoculated eggs were then heated by one of several means to a yolk temperature of about 55 C and held at that temperature for varying periods of time. The number of surviving cells was determined. It is possible to obtain a 7 log cycle reduction of SE in inoculated eggs without a significant change in functional or visual quality of the eggs.

Animals↗
PubMed1996

[Experimental study of immunity in anti-plasmodium infection on preserved spleen and splenectomized mice].

OBJECTIVE: To investigate the function of the spleen as well as the changes of splenic function after hemisplenectomy and hemisplenic autotransplanted in plasmodium yoelili infected mice (first and second infection). METHODS: 336 mice were divided into six groups: 1. total splenectomy, 2. hemisplenectomy, 3. hemisplenic autotransplanted, 4. sham-operation, 5. total splenectomy of 2nd infection, 6. sham-operation of the 2nd infection. 3 months after operation (1-4) or 15 day after 1st infection and operation (5-6) in mice intraperitoneal inoculations were initiated. RESULTS: 1. First infection: in total splenectomy group the antiplasmodium antibody reduced, the remanent rate of 51Cr-RBC in circulation was high, RBC count continously decreased, parasitized erytrocytes still were seen after 1 month. These changes were mild in the preserved spleen. But the production of antiplasmodium antibody and spleen uptake rate of 51Cr-RBC of the hemisplenic autotrans aplaned were rather lower than those of the shamoperate group. 2. Second lethal yoelli infection: in the mice of shamoperation of 2nd yoelii infection the parasitized erytrocyte was lower than that of shamoperated group, but the higher level antiplasmodium antibody and uptake rates of 51Cr-RBC of unit weight splenic tissue. CONCLUSIONS: Under the plasmodium infection preserved hemispleen alternate normal spleen to play the immunologic role, splenic tissue autotransplantation can fairly restore the partial function of spleen. Different preserved spleen method is a useful operation for clinic.

Animals↗
PubMed1995

Animal model for maturity-onset diabetes of the young generated by disruption of the mouse glucokinase gene.

Glucokinase catalyzes a rate-limiting step in glucose metabolism in hepatocytes and pancreatic beta cells and is considered the "glucose sensor" for regulation of insulin secretion. Patients with maturity-onset diabetes of the young (MODY) have heterozygous point mutations in the glucokinase gene that result in reduced enzymatic activity and decreased insulin secretion. However, it remains unclear whether abnormal liver glucose metabolism contributes to the MODY disease. Here we show that disruption of the glucokinase gene results in a phenotype similar to MODY in heterozygous mice. Reduced islet glucokinase activity causes mildly elevated fasting blood glucose levels. Hyperglycemic clamp studies reveal decreased glucose tolerance and abnormal liver glucose metabolism. These findings demonstrate a key role for glucokinase in glucose homeostasis and implicate both islets and liver in the MODY disease.

Animals↗
PubMed1995

Molecular analysis of rat dentin sialoprotein.

Dentin sialoprotein (DSP) is a noncollagenous protein originally isolated from rat dentin. Because it is made by odontoblasts that are actively synthesizing dentin. DSP may play an important role in dentinogenesis. We have isolated a full length DSP cDNA from a rat odontoblast/dental pulp cDNA library (Ritchie et al. [1994] J. Biol. Chem. 269:3698-3702) which codes for a 17 residue signal peptide and a 366 residue, 53 kDa mature protein. In situ hybridization revealed DSP mRNA expression by odontoblasts, but no other cells, in jaws from newborn rat. Northern analysis of various rat tissues demonstrated the presence of DSP transcripts in newborn tooth germs and 21 day old rat incisors. Moreover, multiple transcripts of 4.6 kb and 1.5 kb were found in these two tissues. To better understand the origin of these DSP mRNA multiple transcripts, we have isolated two rat genomic clones. Digestion of each clone with EcoRI followed by Southern analysis revealed that DSP cDNA hybridized to a 4 kb fragment in a lambda dash clone and to a 6 kb fragment in a cosmid clone. Since DSP cDNA hybridized to a 6 kb EcoRI fragment and a 4 kb EcoRI fragment obtained from a rat liver genomic cDNA digested with EcoRI, the multiple DSP mRNA transcripts are most likely derived from two related DSP genes which coexist in the rat genome.

Animals↗
PubMed1995

[Computed sonography observation on the effects of cimetidine on the liver blood flow in cirrhotic patients with portal hypertension].

The effects of cimetidine on the hepatic and portal vein blood flow were investigated in 20 cirrhotic patients with portal hypertension by computed sonography. Cimetidine given intravenously at a dose of 0.4g significantly increased the hepatic vein blood flow (60.2% +/- 63.6%, P < 0.01), at the same time its calculated cross section area increased by 40.2% +/- 81.3% (P < 0.01). The portal vein blood flow increased by 53.2% +/- 52.3% (P < 0.01). While its cross section area was 16.8% +/- 17.3% (P < 0.01). These results showed that the histamine H2-receptor antagonist cimetidine may dilate liver vascular bed and reduce portal blood flow resistance in cirrhotic patients with portal hypertension.

Adult↗
PubMed1994

Cloning and sequence determination of rat dentin sialoprotein, a novel dentin protein.

Dentin sialoprotein (DSP) is a 53-kDa protein isolated from rat dentin. It contains 29.6% carbohydrate (including 9% sialic acid) and has an overall composition similar to that of the bone sialoproteins osteopontin and bone sialoprotein (i.e. rich in Asp, Ser, Glu, and Gly). Using a monospecific anti-DSP polyclonal antibody to screen a rat incisor odontoblast cDNA library, a cDNA clone was isolated and sequenced. This approximately 750-base pair clone contained a DNA sequence corresponding to the NH2-terminal 9 amino acids of DSP. A second cDNA clone was isolated by using the first cDNA as a probe to rescreen the library. This second clone had the full-length DSP coding region. From the sequence, we deduced that the DSP cDNA coded for 366 amino acids, predominantly Asp, Ser, Glu, and Gly. The amino acid composition calculated for this sequence was very similar to that for purified DSP reported earlier; likewise the deduced molecular weight (53,045) was essentially identical to that determined by sedimentation equilibrium. Six potential N-linked glycosylation sites were present in the predicted DSP sequence. No Arg-Gly-Asp sequence was found, and the sequence for DSP was dissimilar to those of osteopontin and bone sialoprotein. Multiple transcripts near 4.6 and 1.5 kilobases were detected by Northern blot analysis in the incisor of 21-day-old rat and the tooth germ of the newborn rat. Consistent with previous immunohistochemical findings, no transcripts were detected in brain, salivary gland, heart, muscle, spleen, kidney, intestine, lung, liver, pancreas, tibia, calvaria, or osteoblast-like osteosarcoma (ROS 17/2.8) cells, indicating that DSP is specifically expressed by odontoblasts and related cells.

Amino Acid Sequence↗
PubMed1989

[Clinical studies and ultrastructure in myotonic dystrophy].

The results of clinical and ultrastructural observation on the affected skeletal muscles in myotonic dystrophy are reported. The patients were five males and four females, aged 24-51 years. The symptoms were classical, there are myotonia, muscular atrophy, weakness and other multi-systemic symptoms, etc. Electromyography shown myotonic response. The major features under electron microscope were as follows. The partial sarcomeres shown lysis; there are the sarcoplasmic pads, sarcoplasmic masses; the muscular nuclear are proliferous and its intrude. The posterior membrane of synapse are simplism, etc. The correlations between these ultrastructural changes and pathogenesis were discussed.

Adult↗