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Biomedical subjects

H Inaoka

Publications and source records attributed to H Inaoka.

13 recordsLinked to original sources
PubMed1996

Extracellular polysaccharides produced by tuberose callus.

A high yield of extracellular polysaccharide (ECP) was obtained from callus cultures of tuberose (Polianthes tuberosa), which could be separated into an unadsorbed and two acidic fractions (TPS-1, -2) by ion-exchange column chromatography. The yields of each fraction were markedly increased by the addition of 10(-5) M 2,4-dichlorophenoxyacetic acid to the medium. Of the three fractions, the amount of TPS-1 accounted for over 60% of total yield of ECP, which was a predominant polysaccharide consisting of arabinose (Ara), mannose (Man) and galactose (Gal) as major neutral monosaccharides. Judging from the patterns of electrophoresis and ultra-centrifugation, TPS-1 was identified to be homogeneous. Methylation and GC-mass spectrometry analyses of this fraction revealed the presence of 1,2,3-linked Man, 1-linked Ara, 1,3-linked Ara, 1-linked Gal and 1,3,4-linked glucuronosyl (GlcUA) residues in a molar ratio of 1.0:1.08:0.85:0.75:1.08. Based on additional analyses of the mild acid hydrolysate and the absolute configuration of the constituent monosaccharides, a possible structure for TPS-1 was a glucuronomannan possessing the unit of -->4)-beta-D-GlcUAp-(1-->2)-alpha-D-Manp-(1--> with branching at the C-3 position, where -->1)-alpha-L-Araf, -->1)-beta-D-galp, -->1)-alpha-L-Araf-(3-->1)-alpha-L-Araf or -->1)-alpha-L-Araf-(3-->1)-beta-D-Galp were attached randomly. About 35% of the GlcUA moieties were present as methyl esters. Further confirmation was made by 1H and 13C NMR spectroscopy.

Carbohydrate Conformation↗
PubMed1996

Indirect measurement of tidal volume by multi-lead electrocardiograms: effect of filtering, deep breath, lead and posture.

We examined a new method for estimating tidal volume from the electrocardiogram (ECG) in order to dispense with the need for sensors used exclusively for tidal volume measurement. The direction of the vector loop in the QRS complex (DVL) was defined as the direction of the diagonal line of the rectangle which circumscribed the vector loop obtained from two orthogonal leads. Two leads of the ECG were transformed into the orthogonal leads using Einthoven's triangle. The peak-to-peak change in the direction (the change in the DVL) was highly correlated with tidal volume. Low-pass filtering of the ECG produced a better correlation between tidal volume and the change in the DVL, when the cutoff frequency was set at 20-45 Hz. A tidal volume of less than 1.21 was correlated more highly with the change in the DVL than a tidal volume of more than 1.21 A combination of leads I and III produced the best correlation between tidal volume and the change in the DVL, compared with other combinations. The posture of the subject had some effects on the slope of the linear regression between tidal volume and the change in the DVL.

Electrocardiography↗
PubMed1985

Detection of tetrodotoxin-like compounds in two species of puffer fishes (Lagocephalus lunaris lunaris and Fugu niphobles).

The toxins in the muscle, skin and liver of the puffer fish Lagocephalus lunaris lunaris and those in the skin and liver of another puffer fish Fugu niphobles were purified by successive column chromatography on activated charcoal, Bio-Gel P-2 and Bio-Rex 70 and analyzed by electrophoresis and thin layer chromatography. Although, regardless of the tissues and species, tetrodotoxin was predominant and accounted for more than 90% of the total toxins, the following minor toxins were newly detected; toxin A and toxin B in L. lunaris lunaris and toxin C in F. niphobles. Since these minor toxins, like tetrodotoxin, were positive to the Weber reagent and 10% KOH, but negative to 1% H2O2, they were assumed to be tetrodotoxin-like compounds. Interestingly, the electrophoretic and thin layer chromatographic analyses showed that toxin A and toxin C are probably identical with AFT3 and AFT1, the minor toxins in the crab Atergatis floridus from Chiba, Japan, respectively.

Animals↗