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Biomedical subjects

H Inufusa

Publications and source records attributed to H Inufusa.

At least 19 recordsLinked to original sources
PubMed1996

Second-look operation for recurrent colorectal cancer based on carcinoembryonic antigen and imaging techniques.

PURPOSE: The usefulness of postoperative carcinoembryonic antigen (CEA) monitoring and improvements in imaging techniques have renewed enthusiasm for second-look operations (SLO) as the most effective treatment for recurrent colorectal cancer by reresection following early detection. The aim of our study is to evaluate the role of CEA and imaging techniques-directed SLO. METHODS: Seven hundred fifty-six patients with Dukes Stages B and C, who had undergone curative resection, were monitored postoperatively using CEA and imaging techniques. An SLO was performed on any potentially resectable recurrence, and in addition, an SLO was done when a persistently rising CEA value was detected. RESULTS: Recurrence developed in 18.8 percent (142/756) of patients, and 90.8 percent (129/142) of the recurrences were detected within the first three years following curative resection. When comparing carcinomas of the colon with that of the rectum, the former were associated with significantly more hepatic and intraabdominal recurrences, whereas the latter had significantly more locoregional and pulmonary recurrences. Seventy-two patients underwent SLO. Of these patients, 54.2 percent (39/72) had all of their disease resected, and 1.4 percent (1/72) had no detectable disease at the SLO. Among the 142 patients with recurrence, 71 (50 percent) patients underwent SLO. The resectable group at SLO carried a significantly better survival than the unresectable recurrence group (41.3 vs. 5.2 percent; P<0.01). CONCLUSIONS: Complete removal of colorectal cancer recurrences by SLO, on the basis of postoperative, follow-up CEA and imaging technique findings, results in improved survival.

Actuarial Analysis↗
PubMed1995

Localization of oncofetal and normal fibronectin in colorectal cancer. Correlation with histologic grade, liver metastasis, and prognosis.

BACKGROUND: Expression of oncofetal fibronectin (oncFN) and normal fibronectin (norFN) in colorectal cancer specimens was examined to investigate the correlation between fibronectin localization and histologic grade, liver metastasis, and prognosis. METHODS: Immunohistochemical staining of oncFN and norFN was performed on 99 primary lesions and 12 liver metastases of colorectal cancer. The expression of norFN and oncFN was evaluated by grading the intensity of staining as negative, positive, or strongly positive. RESULTS: Positive staining for oncFN correlated positively with increasing stage. The rate of strongly positive staining for oncFN was 53% for primary lesions with liver metastasis, significantly higher than the oncFN-positive rate of 13% for metastasis free cases (P < 0.05). Liver lesions had an oncFN-positive rate of 92%. The postoperative 5-year survival rate for 51 cases classified as Dukes Stage C was 77.8% for oncFN-negative cases, 36.5% for oncFN-positive cases, and 22.2% for oncFN-strongly positive cases; these rates were significantly different (P < 0.01). Conversely, there was no correlation between norFN and any clinical variable. CONCLUSION: Expression of oncFN is correlated with a poor prognosis of Dukes C colorectal cancer and may serve as a useful postoperative prognostic sign.

Antigens, Neoplasm↗
PubMed1994

[MultiCycle software for cell cycle analyses].

MultiCycle software (M-cycle), a computer cell cycle analysis program that has a background debris and aggregation compensating function, was utilized in this study to prove the usefulness of the M-cycle. The S phase fraction (SPF) calculated by the M-cycle was compared to that of bromodeoxyuridine labelling index (BLI) using colorectal carcinoma cell lines. The SPF value was slightly lower using the M-cycle than that of the BLI in Colo 201 and Colo 320 and lower significantly in Widr. This may indicate that the M-cycle effectively compensated for the background existing in the DNA histogram. The SPF value was computed both by the M-cycle and the sum of broadened rectangles model (SOBR). The SPF value of these cell lines showed a lower figure in the M-cycle than in the SOBR. The SPF value of paraffin-embedded material through the M-cycle and the SOBR was compared according to DNA ploidy patterns. The SPF value computed by the M-cycle was significantly lower in both ploidy patterns than that of the SOBR. In conclusion, the M-cycle is a useful tool for cell cycle analyses of simple DNA flow cytometric histograms obtained by paraffin-embedded material.

Cell Cycle↗
PubMed1993

[Establishment of a human lung squamous cell carcinoma cell line LK-17, and characterization of procoagulant, platelet aggregation and metastatic potential].

A squamous cell carcinoma cell line LK-17 was established from original surgical specimen of the lung. Doubling time of LK-17 in vitro is 43.2 hours, and chromosome analysis shows various abnormality and main modeat 62. LK-17 shows stable metastatic potential to the lung of nude mouse when injected i.v. LK-17 cells show platelet aggregating activity with number population dependent manner. LK-17 secretes direct factor X activating procoagulant which differs from those of tissue factor, cystein protease A and coagulant cancer antigen 1. These platelet aggregation potential and procoagulant activity may play a important roll in metastatic process.

Animals↗
PubMed1992

[Establishment and characterization of a human squamous cell carcinoma cell line LK-52 which produce direct activator of coagulant factor X].

Uterus origin squamous cell carcinoma cell line LK-52 was established from surgical specimen of lung metastatic nodule. LK-52 produce poorly differentiated squamous carcinoma in nude mouse, and doubling time in vitro was 38 hours. Chromosome analysis show various abnormality and main mode number was 67 and 68. LK-52 shed active procoagulant substance into culture medium. The culture medium of LK-52 shortening recalcification time of normal human plasma and factor VII or factor IX deficient plasma but not factor X deficient plasma. Procoagulant activity of LK-52 product may induced with direct activation of coagulant factor X. Procoagulant activity which produced by cancer cell may play a important roll in the unbalanced haemostasis of cancer patient.

Adult↗
PubMed1989

[Establishment and characterization of human lung cancer cell line (KUM.LK-2)].

Human lung cancer cell line, named KUM.LK-2 was established from xenograft implanted in nude mice and maintained for 43 months. And serially transplanted in nude mice. This line has the following biological characters. 1) Spontaneous lung metastases are made in nude mice inoculated subcutaneously. 2) This line can produce some kinds of proteases, like as tissue type plasminogen activator, urokinase type plasminogen activator and collagenase. 3) KUM.LK-2 has the ability of platelet aggregation.

Adenocarcinoma↗
PubMed1988

Human serum bilirubin fractionation in various hepatobiliary diseases by the newly developed high performance liquid chromatography.

Serum Bilirubin was fractionated by newly developed reversed phase high performance liquid chromatography (HPLC) into 5 fractions: delta (delta-Bilirubin, B delta), gamma (bilirubin diglucuronide, BDG), beta (Bilirubin monoglucuronide, BMG), beta' ((Z, E,)- and/or (E, Z)-bilirubin IX alpha) and alpha ((Z, Z)-bilirubin IX alpha). Sera of healthy subjects and of patients with unconjugated hyperbilirubinemia showed predominantly alpha fraction with a small amount of beta' fraction. Trace amounts of delta fraction were detected in a few cases. The results of fractionation of serum bilirubin in 159 patients with various hepatobiliary diseases suggested that the ratios B delta/(B delta + BDG + BMG) and BMG/B delta can be useful parameters to follow patients with jaundice, compared with the reported B delta/total bilirubin which did not always reflect the jaundice stage, especially in cases with low serum bilirubin levels.

Biliary Tract Diseases↗
PubMed1988

Serum bilirubin fractions in analbuminemic rats after bile duct ligation. Albumin requirement in the formation of covalently protein-bound bilirubin.

To clarify the role of albumin in the production of covalently protein-bound bilirubin (delta bilirubin, B delta) in the blood and the pathophysiological relevance of B delta, changes in the serum bilirubin level and histological findings in the liver, kidneys and myocardium were studied in Sprague-Dawley (SD) rats and Nagase analbuminemic rats (NAR) after bile duct ligation (BDL). In SD rats, the serum bilirubin level increased until 3 days after BDL, and was followed by the appearance of B delta. The increase in serum bilirubin was smaller in NAR, and no serum B delta was noted. Albumin administration to NAR 1 day after BDL increased serum bilirubin with the appearance of B delta. Serum bilirubin decreased in both SD rats and NAR 7 days after BDL. Marked deposition of bile pigments was noted in NAR in the renal tubular epithelium. The renal bilirubin content was decreased after albumin administration. From these results it is concluded that albumin is necessary for the production of B delta, and that renal deposition of bile pigments progresses in the absence of albumin.

Animals↗
PubMed1988

[Establishment and characterization of human lung adenocarcinoma cell line KUM.LK-2 which produces spontaneous lung metastasis by subcutaneous implantation in nude mice].

Characterization were newly established at the human lung adenocarcinoma cell line KUM.LK-2 which produced spontaneous lung metastasis by subcutaneous implantation in nude mouse. KUM.LK-2 cell line was established from the 2nd generation of nude mouse serially transplanted human lung adenocarcinoma. KUM.LK-2 produced spontaneous lung metastasis when cells were implanted into subcutaneous tissue of nude mice. Metastasis was not found in the cases of intramuscular implantation and intravenous injection of these cells. KUM.LK-2 cell produced Carcinoembryonic Antigen and Carbohydrate Antigen 19-9 in vitro, but these tumor markers were not detected in the sera of KUM.LK-2 transplanted nude mice. From this study the model of human cancer with spontaneous lung metastasis was established. It was suggested that metastasis of human cancer in the nude mouse could not be determined by blood supply and new vascular genesis only. And it was concluded that usefulness of nude mice as an amplifier of tumor marker is varied with tumor lines.

Adenocarcinoma↗
PubMed1988

[Influence of a fosfomycin combination on the anti-tumor effect of CDDP].

To elucidate the influence of fosfomycin (FOM) on the anti-cancer effect of cisplatin (CDDP), experimental chemotherapy of CDDP with or without FOM was performed. A human lung cancer cell line, HLC-1 serially transplanted into nude mice was treated with CDDP 10 mg/kg (i.p.). Tumor doubling time in CDDP group and CDDP + FOM group are 26.78 +/- 6.3 days and 33.90 +/- 11.82 days respectively, and they are significantly elongated compared to control group. Serum NAG level in CDDP + FOM group was significantly lowered. Therefore, we concluded that anti-tumor effect of CDDP was not diminished by FOM combination.

Adenocarcinoma↗
PubMed1988

Clinical application of serum bilirubin fractionation by simplified liquid chromatography.

Serum bilirubin was fractionated by a new reversed-phase "high-performance" liquid-chromatographic (HPLC) procedure, on Micronex RP-30, a polyacryl ester. The five fractions were: delta (delta-bilirubin, B delta), gamma (bilirubin diglucuronide, BDG), beta (bilirubin monoglucuronide, BMG), beta' [(Z,E)- and (or) (E,Z)-bilirubin IX alpha], and alpha [(Z,Z)-bilirubin IX alpha]. We found close correlation with results of the modified HPLC fractionation of Lauff et al. (J Chromatogr 1981;226:391-402), except for the beta' fraction, which was eluted after beta. The Micronex HPLC involves a simple pretreatment of serum samples, in contrast with the complex preparation described by Lauff et al., and is convenient for routine use in the clinical evaluation of hyperbilirubinemia. We could quantify B delta, BDG, BMG, and unconjugated bilirubin even in sera with normal values for total-bilirubin concentrations. Photoderivatives of bilirubin such as the beta' fraction could be separated and quantified by the same procedure, making the method feasible for pediatric research.

Bilirubin↗
PubMed1987

Serum bilirubin fractionation using multilayer film method in hepatobiliary diseases in comparison with high performance liquid chromatography.

The Ektachem multilayer film method (Ektachem) and high performance liquid chromatography (HPLC) were employed to fractionate and evaluate serum bilirubin species in 45 serum samples. The false-positive or false-high levels of bilirubin close-bonded with albumin (i.e. the delta bilirubin fraction (B delta] was obtained by Ektachem in sera of cases with normal bilirubin concentration and cases with unconjugated hyperbilirubinemia when compared with the results of HPLC. In the sera of cases with conjugated hyperbilirubinemia, Ektachem gave comparable levels of total bilirubin (TB), and unconjugated bilirubin (Bu) to those of HPLC, but underestimated conjugated bilirubin (Bc) and slightly overestimated B delta. To investigate the clinical significance of B delta in 113 cases of various hepatobiliary diseases with conjugated hyperbilirubinemia, the ratios of B delta to TB (B delta/TB) and to directly-reacting bilirubin fractions (B delta/(Bc + B delta] and that of Bc to B delta (Bc/B delta) were calculated based on the results of Ektachem and compared with each other during the course of jaundice. The mean B delta/TB was below 40% in various hepatobiliary diseases but became as high as approximately 60% in the convalescence stage. The mean B delta/(Bc + B delta) was below 50% in acute hepatitis (the serum bilirubin-elevating stage) and obstructive jaundice, and it increased to above 80% in the recovery stage. In decompensated liver cirrhosis and intrahepatic cholestasis the mean B delta/(Bc + B delta) was about 60%, indicating continuous backflow of Bc from liver cells. The changes in B delta/(Bc + B delta) were much greater than in B delta/TB.(ABSTRACT TRUNCATED AT 250 WORDS)

Biliary Tract Diseases↗