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Biomedical subjects

H Irie

Publications and source records attributed to H Irie.

At least 19 recordsLinked to original sources
PubMed1992

Angiokeratoma of the clitoris: a subtype of angiokeratoma vulvae.

A 24-year-old married woman had an angiokeratoma of the vulva measuring 3 x 3 x 4 mm localized at the clitoris. The nodule was noticed when she was pregnant with her first child. This tumor grew with occasional tenderness and bleeding during pregnancy, but its size did not change after delivery. The growth of this tumor might be attributable to an increase in venous pressure or serum progesterone levels; therefore this disorder might be classified as a subtype of angiokeratoma of the vulva. This is the first case of angiokeratoma localized at the clitoris.

Adult↗
PubMed1992

Efficacy of oral administration of live herpes simplex virus type 1 as a vaccine.

Mice given herpes simplex virus type 1 (HSV-1) (Miyama +GC strain) intragastrically via a stainless-steel cannula were rendered immune to subsequent lethal intraperitoneal (i.p.) challenge with HSV-1. The orally administered HSV-1 was completely inactivated in the stomach within a few minutes of inoculation. However, systemic immunity was established 14 days after oral inoculation with the virus and retained for up to 6 months. The mechanisms of establishing systemic immunity were investigated by means of adoptive transfer comparisons. When splenic cells from HSV-1-immunized mice were transplanted into nonimmunized mice, all of the recipient mice survived after a lethal i.p. challenge with the virus. Immunity was not established in antithymocyte serum-treated mice or by transfer of serum from immunized to nonimmunized mice. In addition, all HSV-1-immunized mice died after lethal challenge with HSV-2 and influenza virus A. These findings suggest that the immunity was virus specific, with T lymphocytes playing a major role in its establishment. The present study therefore supports the possibility of oral immunization with live HSV-1 as a vaccine.

Administration, Oral↗
PubMed1992

Mechanical circulatory support with a centrifugal pump after open heart surgery.

Since December 1988, a centrifugal ventricular assist device (VAD) was used to support the circulation in 5 patients who could not be weaned from cardiopulmonary bypass (CPB) or developed cardiogenic shock after removal from CPB. Three patients required a left VAD, one needed a right VAD. One patient had biventricular support using a centrifugal left VAD and a diaphragm type right VAD. The duration of the centrifugal VAD support ranged from 6 to 136 (mean 72)h. All patients were weaned from the VAD, but only 2 patients were discharged from the hospital. Two patients died of multiple organ failure, and one died of cardiogenic shock caused by intractable arrhythmia. Infection occurred in all non-survivors, and 2 of them developed renal failure. We conclude that the centrifugal VAD is effective to recover a failing ventricle. The factors related to the unsuccessful recovery were delayed start of the VAD support and major complications such as infection as infection and renal failure.

Adult↗
PubMed1992

Hemodynamic performance of the biventricular bypass system operated in an independent variable rate mode.

The present study was undertaken to determine whether a biventricular bypass system operated in an independent variable rate (VR) mode can maintain the entire circulation. Two pusher-plate pumps which incorporated the Hall effect position sensors were used to bypass the right and left ventricles in 10 sheep under fibrillation. The flow distributions of the pump output to the carotid and renal arteries were investigated every 6 h using ultrasonic blood flow meters for 24 h in 5 animals, and the controllability of the VR mode was evaluated in 5 long-term experiments. The carotid artery flow ratio to the pump output decreased significantly from 4.7 +/- 0.8% before the bypass to 2.7 +/- 0.9% after 24 h. However, the renal artery flow ratio did not change throughout the experiments. In the long-term experiments, the animals were kept alive from 3 to 48 days (mean 15.6 days). The mean pump output had been maintained at more than 90 ml/min/kg for the first 7 days. After the surgery, the pump driving conditions were not readjusted in any experiment. The results indicate that the biventricular bypass system operated in the independent VR mode automatically maintains the entire circulation at a satisfactory level.

Animals↗
PubMed1991

[MRI of brain abscesses].

Four cases of brain abscess were examined on 1.5T MR and their findings were evaluated retrospectively. Preoperative cases had relatively characteristic findings, such as 1) peripheral edema producing hypointensity on T1-weighted images (T1WI) and hyperintensity on T2-weighted images (T2WI); 2) central necrosis with abscess fluid hypointense relative to white matter on T1WI and hyperintense on T2WI; 3) abscess rim iso- to hypointense on T2WI. Gd-DTPA enhanced T1WI carried further information; comparison of iso- to hypointense rim on T2WI (abscess rim) and enhanced area by Gd-DTPA on T1WI suggested that the former represented zone of macrophage infiltration.

Adult↗
PubMed1991

[Main pulmonary artery injury caused by golf-swing].

A 87-year-old man suddenly complained of dyspnea and fell into shock just after tee-shot. UCG revealed a large amount of pericardial effusion and good wall motion of the heart. Enhanced CT scan didn't prove any intimal flaps in the aorta. With cardiopulmonary bypass stand-by, we opened the pericardium and found a small laceration in the main pulmonary artery. The site of the laceration corresponded to a hard and sharp pericardial reflection ridge. We speculated that the difference of deceleration power between body and the heart resulted in pendulous or torsional motion of his heart. Supposedly the mutual actions between sharp pericardial reflection ridge and strange motion of the heart injured his main pulmonary artery.

Aged↗
PubMed1990

Suppression of actively induced and passively transferred experimental allergic neuritis by cyclosporin A.

The effect of cyclosporin A (CyA) on experimental allergic neuritis (EAN) was examined. CyA suppressed the development of actively induced EAN. The earlier treatment with CyA was begun, the more effective was its effect. CyA was effective even after the development of clinical signs. In actively induced EAN, cessation of CyA treatment lead to the development of EAN. However, in passively induced EAN, it did not. We could not find any differences in the distribution of infiltrating T cell subsets between the control group and the CyA-treated group.

Animals↗
PubMed1990

Prevention of immunological disorders in MRL/l mice by a new synthetic analogue of vitamin D3: 22-oxa-1 alpha,25-dihydroxyvitamin D3.

We examined the immunoregulating effect of 22-oxa-1 alpha,25-dihydroxyvitamin D3 (22-oxa-1 alpha,25(OH)2D3), a synthetic analogue of vitamin D3 with an oxygen atom at C22 in the side chain skeleton, on spontaneously developing autoimmune disorders in MRL/Mp-lpr/lpr (MRL/l) mice. The oral administration of the compound significantly prolonged the average life span of the mice and showed a significant reduction in proteinuria. Histopathological investigations also revealed that pathological conditions such as renal arteritis, granuloma or arthritis of the knee joints were much lighter in the treated group than in the untreated group. Furthermore, the lymphocyte phenotypes in thymus, lymphnode, and spleen were partially normalized and became similar to those found in young control animals by the treatment with 22-oxa-1 alpha,25(OH)2D3. These results suggest that this compound inhibits the development of lupus nephritis in MRL/l mice and may be therapeutically effective on the mice.

Animals↗
PubMed1990

[Mesenteric hematoma; a pediatric case].

A 3-Year-old girl was admitted to National Beppu Hospital due to abdominal mass. US examination revealed cystic mass with multiple scattered nonreverberatory echoes in right upper abdomen. CT examination revealed almost homogeneous mass (29-31 H.U.) with a few internal components (36-42 H.U.). US and CT were useful methods for diagnosis of mesenteric hematoma.

Child, Preschool↗
PubMed1990

[Repetitive rhythmic spikes in epileptic children].

Repetitive rhythmic spikes (RRSs') sometimes appear periodically for 0.2-2.0 seconds at localized in epileptic children. We selected 11 patients (male 6, female 5) with RRSs for this study. Ages were 3-15 years (average 10.7 years). We counted the number of spikes which were recorded on the scalp EEG. The duration of RRSs and the differences of amplitude between large and small spikes on monopolar montages were examined. We also studied the appearance of spikes under different recording conditions; waking and sleep stages, photic stimulation and hyperventilation. Furthermore, we made two dimensional joint-dot plotting for the interval between repetitive spikes. RRSs were classified into two groups. In the first group, joint-dots of RRSs were locally observed between 0.2-0.8 sec. In the second, they were disseminated between 0.2-2.0 sec. In two groups, spikes were mainly observed in the sleep EEG (stage 1). The average number of spikes in the first group was 18.7/min, and that in the second was 25.7/min. The average difference of the amplitude between large and small spikes was 103 microV in the first group, and 88 microV in the second group. The average duration of spikes was 54.2 msec in the first group and 62.4 msec in the second group. From these results, it was assumed that some of spikes in the first group occurred in the surface of the cortex and that some of spikes in the second group were projected from the deep region in the cortex.

Adolescent↗
PubMed1990

[Intracranial multiple granuloma preceded by rheumatic disease--a case report].

In 1980, a 38-year-old man had remittent fever, swelling and arthralgia of the knee, ankle and wrist joints, as well as visual disturbance due to bilateral iritis. On his admission to our hospital, his laboratory data showed neutrophilia, normocytic normochromic anemia, hepatomegaly, hepatocellular damage, and a strongly positive RA test. All the microbiological examinations were negative. Thirty mg of prednisolone improved his symptoms and abnormal laboratory findings. Due to persistent mild arthralgia, he had continued to take 5-10 mg of prednisolone and analgesics until 1985 when he was readmitted to our hospital. In 1983, he began to complain of a steady pain around his left eye, and he sometimes had double vision. In 1985, he began to complain of decreasing left visual acuity and sensory disturbance in his left face in addition to pain in and around the eye. On his 2nd admission to our hospital, the neurological examination revealed involvement of the 2nd and 3rd cranial nerves and the 1st branch of the 5th cranial nerves of the left side. Laboratory data showed a positive RA test with RAHA titer at 1:320 and IgM at 216 mg/dl, but he had no joint deformities. The computed tomography (CT) of the brain demonstrated a high density mass of his left cavernous sinus extending to the left orbital apex. The prescription of the high dose of prednisolone (100 mg/day) relieved ophthalmic pain and improved visual acuity and neurological involvement within a week. Prednisolone was then gradually decreased to 10 mg. In 1986, he had partial and complex partial seizures.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1990

[Assay of nuclear triiodothyronine receptors in the precentral gyrus in patients with amyotrophic lateral sclerosis].

Abnormality in the receptor of the cell might have some bearing on the selective cellular damage in systemic degenerative diseases. In relation to deranged metabolic turnover of the folate cycle in amyotrophic lateral sclerosis (ALS), we have measured nuclear triiodothyronine receptors (NT3R) in the precentral gyrus, stored at -80 degrees C after autopsy, of 4 ALS patients and 8 patients with non-neurological diseases. Cell nuclei were separated in bulk from about 1 g of grey matter by the method of Løvtrup-Rein & McEwen, and T3-binding assay was carried out by the method of Silva et al. DNA was measured by the method of Giles & Myers based on Burton's method. Specific bindings comprised more than 85% of total bindings. Scatchard analysis showed that the maximal binding capacity, namely the density of NT3R was significantly reduced in ALS, compared to that of the controls. The dissociation constant, namely the affinity was not different between the two groups. The present investigation suggests that NT3R may be reduced in the motoneuron of ALS patients, although the result might merely indicate the loss of nerve cells from the ALS motor cortex. This point should be clarified, but the bulk separation of pure nerve cell nuclei from autopsied human brain has not so far been successful in our laboratory. Action of thyroid hormone in the mature brain has not fully been clarified. Meanwhile, some investigators suggest that the metabolic state of the central nervous system of ALS has a tendency of hypothyroidism. Therefore, the relevance of thyroid hormone to pathogenesis or pathophysiology of ALS will be an important subject in future study.

Aged↗
PubMed1989

Spread of herpes simplex virus type-1 (Miyama +GC strain) to the central nervous system after intraperitoneal inoculation: the role of the myenteric plexus of the gut.

The pathways taken by the HSV-1 virus after intraperitoneal (i.p.) inoculation were studied in 5-week old male C3H/HeN mice injected with 1 x 10(4) PFU (100 LD50) or 5 x 10(5) PFU (5000 LD50) of HSV-1 (Miyama +GC strain). At the higher dosage (5 x 10(5) PFU), HSV-1 began replicating in the adrenal from the first day, then in the gut and thoracic portion of the spinal cord by the third day, and in the brainstem by the fourth day, as shown by the titers of the virus in these organs. By immunoperoxidase staining HSV-1 was localized in a necrotic area of the adrenal, the myenteric plexus of the gut, the intermediolateral columns of the thoracic cord, and the vagus nerve nuclei of the medulla oblongata. In the low dose mice (1 x 10(4) PFU), HSV-1 was not isolated from the adrenal or thoracic segment of the spinal cord from the time of inoculation until the time of death. It was, however, isolated from the gut on days 4-6 and from the brainstem by day 5. HSV-1 was never isolated from the blood of either group at any time. The localizations of viral replication suggest that in the mice inoculated with 1 x 10(4) PFU, HSV-1 spreads to the brainstem via the vagal nerves after replication in the myenteric plexus of the gut. In the mice given the higher dose, localizations suggest not only the above route, but also that the virus spread to the intermediolateral columns of the spinal cord after replicating in the adrenal.

Animals↗
PubMed1989

Protection of OK-432, a Streptococcus pyogenes preparation, against lethal infection of mice with herpes simplex virus.

We have studied the protective effect of OK-432, a biological response modifier (BRM) of Streptococcus pyogenes origin, on the lethal infection of mice with herpes simplex virus (HSV)-1. A single intraperitoneal (i.p.) injection of more than 10 micrograms of OK-432, when given at least two days before the infection, gave a marked effect yielding nearly 100% protection against ordinarily lethal infection. The protection was independent of the amount of infected virus inoculated. When given after the infection, the agent even at the maximal dose (100 micrograms), produced only a marginal effect. A single i.p. administration of OK-432 augmented the natural killer (NK) activity of peritoneal exudate cells and spleen mononuclear cells in mice 2 to 3 days after injection of OK-432, coinciding with the times when it induced a survival effect on HSV-infection. Treating OK-432-treated mice with a combination of an anti-macrophage agent, silica, and an anti-NK cell agent, anti-asialo GM1 serum, before infection diminished the antiviral effect of OK-432. The OK-432 protection against HSV infection was also markedly diminished in athymic nude mice. Thus, the protective effect of OK-432 on lethal HSV infection seems to be based on the activation of NK cells, macrophages, and T lymphocytes.

Animals↗
PubMed1989

[Application of beta-tricalcium phosphate to periodontal therapy. Part 1. Fundamental studies and biological tests of beta-TCP prepared by mechanochemical method].

Physical, chemical, biological and implant tests on calcium phosphate prepared by the mechanochemical method with wet milling were performed to determine their applicability for periodontal therapy. 1. Materials synthesized by the new method had a stoichiometric ratio of Ca/P = 1.50 and a density of 3.70 g/cm3 and coincided with the standard pattern of beta-TCP (ASTM Card) very well. 2. The surface structure of dense type sintered at 1,100 degrees C showed fewer fine pores than the dense type sintered at 1,050 degrees C. The surface structure of the porous type sintered at 1,050 degrees C showed finer pores, except for a continuous large pore than the porous type sintered at 1,000 degrees C. 3. Specific conductivity tests should higher solubilities of the porous type than of the dense type in pure water, and of materials sintered at low temperature than at high temperature. 4. Cytotoxicity studies with V-79 cells showed no significant differences from the controls, and other biological tests revealed no abnormalities. 5. At three and six weeks after implantation in muscles, all of beta-TCP was enclosed with collagen fiber. The porous type of beta-TCP was almost completely fused to cranical bone in rabbits three weeks after implantation. The dense type of beta-TCP was completely fused to cranical bone six weeks after implantation. From these results, it can be concluded that beta-TCP prepared by the new method is of high purity and biocompatibility. Consequently beta-TCP is expected to be effective in clinical use.

Biocompatible Materials↗
PubMed1989

Dot microfiltration sandwich EIA for the measurement of IGM concentrations in cerebrospinal fluid.

A sensitive and simple sandwich enzyme immunoassay (EIA) using a dot microfiltration apparatus has been developed for the measurement of immunoglobulin M (IgM) concentrations in cerebrospinal fluid (CSF). The lower detection limit was 0.5 micrograms/dl. We applied this method to the testing of 200 CSF samples with normal concentrations of albumin, IgG and total protein, and normal cell counting. The non-parametric 2.5 and 97.5 percentile limits of IgM and the IgM index were 6.1-64.3 micrograms/dl and 0.012-0.063, respectively. We also applied this method to the testing of samples from 11 cases of multiple sclerosis (MS) and 15 cases of Guillain-Barré syndrome (GBS). High values for IgM and the IgM index were observed in 18.2% and 9.1% of MS, respectively and in 66.6% and 20.0% of GBS, respectively.

Evaluation Studies as Topic↗