Rat intrinsic factor was bound to vitamin B-12-Sepharose to produce intrinsic factor-vitamin B-12-Sepharose. Intestinal receptor for intrinsic factor-vitamin B-12 complex was purified from rat ileal extract by affinity chromatography using the intrinsic factor-vitamin B-12-Sepharose as an affinity adsorbent with recovery of 48.5% and specific activity increased 335 fold of original sample.

Animals↗

PubMed1977

Ultrastructural investigation of the effect of mitomycin C on the rat exocrine pancreas.

In order to clarify the pathophysiology of digestive disorders which are caused with anticancer agents, Mitomycin C was intravenously administrated to rats and the ultrastructure of the pancreas was studied. The alteration of acinar cells after under 4 daily administrations of 1.0 mg/Kg of Mitomycin C was not remarkable and in an early stage returned to normal structure. In above 8 daily administrations of 1.0 mg/Kg of Mitomycin C, however, pronounced degeneration of acinar cells was induced and acinar cells did not recover to the previous level, but die within one month. The ultrastructural changes induced by Mitomycin C were mainly aggregation of chromatin in the nuclei, tubularly dilatation of the rough endoplasmic reticulum, and swelling of the mitochondria. It was surmised that the biosynthesis and supply of protein such as digestive enzymes were not amply carried on; hence, the ehcmotherapy could give rise to severe digestion disorders. It appeared necessary to further study the dosage and dosage schedule of the anticancer agents, along with the necessity for taking ample care of patients presenting such disorders.

Animals↗

PubMed1977

[Experimental studies on the mechanism of exocrine pancreatic secretion, with special reference to pancreatic secretory response to various stimuli and the effect of atropine].

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Animals↗

PubMed1976

Application of Duhamel's operation for the surgical treatment of rectal cancer.

Several sphincter-preserving procedures for surgical removal of cancer at the upper or middle part of the rectum often cause disturbances of the ano-rectal and bladder functions. To preserve these functions it is important to minimize the damage to the anal sphincter as well as to the pervic nerves and to preserve the rectal mucous membrane as much as possible. Duhamel's procedure was employed with very satisfactory results.

Adult↗

PubMed1976

Effects of anticancer agents of the intestinal epithelium. A morphologic study.

In order to make clear the pathophysiology of digestive symptoms which are caused with anticancer agents, three anticancer agents, i.e., mitomycin C, 5-FU and cytosine arabinoside were administered pre-operatively, and the ultrastructure of the intestinal epithelium, especially that of absorptive cells, were studied on the specimens collected intra-operatively. In view of the finding that severe degeneration occurred inside cytoplasms, chiefly their nuclei, it was surmised that the biosynthesis and supply of proteins such as digestive enzymes were not amply carried on; hence, the chemotherapy could give rise to severe digestion and absorption disorders. It appeared necessary to further study the dosage and dosage schedule of the anticancer agents, along with the necessity for taking ample care of patients presenting such disorders.

Antineoplastic Agents↗

PubMed1976