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Biomedical subjects

H J Andersen

Publications and source records attributed to H J Andersen.

At least 37 records · Page 2Linked to original sources
PubMed1994

Hemichrome formation, lipid peroxidation, enzyme inactivation and protein degradation as indexes of oxidative damage in homogenates of chicken kidney and liver.

The change in relative hemichrome formation (RHF) was investigated as a potential marker of oxidative damage in kidney and liver homogenates prepared from chicks fed diets deficient or adequate in vitamin E. RHF gave an earlier indication of oxidative damage in tissue homogenates than the formation of thiobarbituric acid reactive substances (TBARS) or decrease in glutathione peroxidase activity (GPXA). RHF correlated significantly with both TBARS and GPXA. The correlations were 0.64 (P < 0.0001) and -0.57 (P = 0.0002) in kidney homogenates and 0.53 (P = 0.0006) and -0.71 (P < 0.0001) in liver homogenates. The correlation between RHF and the sum of TBARS and GPXA was also highly significant in both kidney and liver homogenates.

Animals↗
PubMed1993

Protection by vitamin E, selenium, and beta-carotene against oxidative damage in rat liver slices and homogenate.

Male Sprague-Dawley (SD) rats were fed a vitamin E and selenium deficient diet and diets supplemented with vitamin E, selenium, beta-carotene, and a combination of the three. Tissue slices and homogenate of liver were incubated at 37 degrees C with and without the presence of prooxidants. The effect of vitamin E, selenium, beta-carotene, and the combination of the three antioxidants on the oxidative damage to rat liver tissue was studied by measuring the production of oxidized heme proteins in both tissue slices and homogenate during spontaneous and prooxidant-induced oxidation. The diet with the combination of all three antioxidants showed a strong protective effect against oxidative damage to heme proteins in contrast to the antioxidant-deficient diet. In general, diets with vitamin E, selenium, and beta-carotene were less effective than the combination of all three antioxidants. The protective effect of antioxidants on the heme protein oxidation was correlated with their inhibitory effect on lipid peroxidation measured as the production of thiobarbituric acid-reactive substance (TBARS). The protection of antioxidants on heme proteins was also dependent on the type of oxidation inducer. Possible mechanisms of antioxidants against oxidation in liver tissues are discussed.

Animals↗
PubMed1993

Ferrous-iron-induced oxidation in chicken liver slices as measured by hemichrome formation and thiobarbituric acid-reactive substances: effects of dietary vitamin E and beta-carotene.

Hemichrome formation in chicken liver slices was determined by employing a Heme Protein Spectra Analysis Program (HPSAP) on the visible spectrum of the liver tissue. Relative hemichrome formation (RHF) in liver tissue exposed to ferrous iron for 1 h at 37 degrees C could be predicted according to the general catalytic equation RHF = k.[Fe2+]/(Ap + [Fe2+]), with k = 132 +/- 30, where the factor Ap represents the additive antioxidative potential in the liver tissue. RHF in Fe2+ exposed liver slices incubated at 37 degrees C for 1 h correlated significantly with formation of thiobarbituric acid-reactive substances (TBARS) (r = .77, P < .0001). RHF was found to decrease significantly with increasing vitamin E concentration in liver tissue exposed to ferrous iron (1 h, 37 degrees C). However, the influence of beta-carotene on RHF in ferrous-iron exposed liver slices (1 h, 37 degrees C) was less evident, as the concentration of Fe2+ was found to be decisive for whether beta-carotene acted as an antioxidant or a prooxidant under the conditions in question. Results in the liver slice model system regarding the effect of vitamin E and beta-carotene on iron overload were supported in a subsequent in vivo iron injection experiment with chicks. These observations indicate that RHF is a sensitive marker for ferrous-iron-induced oxidative damage in the present tissue slice system.

Animals↗
PubMed1992

A seasonal eosinophilic dermatitis in cats.

Twenty cats from Nelson with distinctive crusting and erosive dermatitis of the nasal bridge and histological lesions of eosinophilic dermatitis, often with collagenolysis, were examined in 1990 and 1991. Four of the cats also had pinnal dermatitis and five had eruptive lesions on the chin. The condition was intermittent and seasonal, occurring in summer and autumn. It is probable that the lesions were caused by hypersensitivity to insect bites, as has been demonstrated in a similar clinicopathological syndrome recently recognised in Australia.

Journal Article↗
PubMed1992

Habits and rituals.

On-going restructuring in the Danish care for the elderly is to a very high degree based on recognition of the significance of habits and rituals, as well on the knowledge of what is needed to alter them. Habits and rituals are concepts that are often confused in everyday patterns of thought. It is necessary to be able to distinguish between the two concepts before change can be effected. To varying degrees people are characterised by unconscious and conscious habits as well as inherited and personal rituals. Personal development, age, support from one's surroundings, and so on, determine the degree to which a person may continue or alter acquired patterns of habit and ritual.

Adaptation, Psychological↗
PubMed1990

[Treatment with rectal diazepam or acetylsalicylic acid in effervescing tablets in ambulatory cervical curettage and biopsy. A double-blind randomized study].

A double-blind randomised investigation with placebo control was undertaken to investigate the effect of 10 mg diazepam rectally or 1 g acetyl salicylic acid as effervescing tablets on pain and nervousness during and after outpatient cervical curettage and biopsy from the vaginal cervix or the cervical endothelium. A total of 97 women participated and were subdivided at random. Three patients were, however, excluded as they did not participate as planned. No differences were found between the three groups as regards pain and nervousness during the intervention and fatigue and drowsiness after the intervention. No statistically significant difference was found in the degree of satisfaction with the treatment in the various therapeutic groups.

Administration, Rectal↗
PubMed1990

Development of rancidity in salmonoid steaks during retail display. A comparison of practical storage life of wild salmon and farmed rainbow trout.

The quality of wild salmon and farmed rainbow trout from aquaculture, both packed in transparent vacuum-skin packaging, was followed during storage for 6 months in an illuminated freezer cabinet (product temperature -17 degrees C, half of the packs protected against light, and half of the packs fully exposed to light), combining (a) colour determination of the carotenoid-pigment flesh by tristimulus colorimetry, (b) determination of thiobarbituric-acid-reactive substances (TBA value), (c) carotenoid analysis and, at the end of the storage experiment, (d) sensory evaluation. Rancidity developed faster in steaks of wild salmon (TBA increased during 6 months of storage from 2.8 mumols malonaldehyde/kg flesh to 12.5 mumols/kg for light-protected packages, and to 17.6 mumols/kg for packages exposed to fluorescent light) as compared to steaks of farmed rainbow trout (TBA increased from 1.2 to 5.8 mumols/kg, independent of light exposure), a finding also confirmed by sensory evaluation. In both products, the carotenoid pigment was identified as astaxanthin; salmon steaks, the product more susceptible to developing rancidity, had the lower astaxanthin content (rainbow trout 9.1 mg/kg flesh, salmon 4.9 mg/kg, prior to storage). While the astaxanthin content remained virtually constant in salmon steaks during storage, the content decreased significantly in steaks of rainbow trout, an observation which suggests the role of astaxanthin as a sacrificial protector against radical processes.

Animals↗
PubMed1989

[Postmenopausal estrogen therapy. Recent aspects of parenteral administration of 17-beta estradiol].

Oral treatment with micronized 17-beta-oestradiol or oestradiol valerate in daily doses of 2 mg postmenopausally resulta in plasma concentrations of free oestrone which are more than double the concentrations in fertile women and plasma oestradiol concentrations which only comprize 20-25% of the oestrone concentrations. On oral treatment, the metabolism in the liver is influenced and this results in increased synthesis of a series of plasma proteins in the liver and changes in lipid and lipoprotein pattern with a fall in serum cholesterol and low density lipoprotein cholesterol and increase in high density lipoprotein cholesterol. Parenteral administration of 17-beta-oestradiol results in plasma concentrations of oestradiol corresponding to those in fertile women. Parenteral administration may take the form of: 20-100 mg oestradiol "pills" implanted subcutaneously, percutaneous administration of 0.05-0.1 mg oestradiol-containing capsules stuck to the skin every 24 hours, 3 mg daily or every second day in a water-alcohol gel, vaginally in cream, suppositories of impregnated vaginal synthetic material (Silastic) rings in daily doses of 0.5-0.25 mg. Percutaneous administration in gel from and partially with vaginal administration in cream or suppositories result in not inconsiderable diurnal variation and inter-individual variation in the plasma oestradiol concentrations obtained. In all of the cases, a relationship is found between the oestradiol and oestrone concentrations which are greater than or equal to one. Implantation treatment is associated with a considerable risk of overtreatment on account of increasing plasma oestradiol concentrations on repeated implantations.(ABSTRACT TRUNCATED AT 250 WORDS)

Administration, Cutaneous↗
PubMed1989

[Progesterone and gestagen treatment. Pharmacologic and clinical aspects].

A review is presented of progestogens which assume a central position in future replacement therapy. The types and dosages of progestogens are of considerable significance for the effects and side effects. Attention is, therefore drawn to the plasma concentration and the minimal effective doses in the following two clinical treatments. Oestrogen primed endometrium is correctly transformed after treatment for 12 days with the following daily dosages: Dydrogesterone = 20 mg, medroxyprogesterone = 10 mg, norethisterone/norethisterone acetate = 0.7 mg, desogestrel = 150 micrograms or levonorgestrel approximately 100 micrograms. Inhibition of ovulation and contraception are obtained by means of the following daily doses: Medroxyprogesterone = 10 mg, norethisterone/norethisterone acetate = 350 micrograms, levonorgestrel = 50 micrograms, desogestrel = 60 micrograms, gestodene = 40 micrograms or norgestimate 250 micrograms.

Chemical Phenomena↗
PubMed1989

A long-term follow-up of the effect of repeated corticosteroid injections for stenosing tenovaginitis.

Ninety-three consecutive adult patients with stenosing tenovaginitis of 104 digits have been treated by corticosteroid injections repeated at three week intervals up to thrice in partial responders. At follow-up after 3-15 years, partial relapse had occurred in two patients, who now and then experienced triggering but without pain or locking. Complete relief of symptoms was obtained in 76%. There were no complications and the success rate was independent of sex, age, duration of symptoms, the digit involved, or associated diseases. These results are superior to those previously reported following a single injection.

Adult↗
PubMed1989

The ischaemic leg: a long-term follow-up with special reference to the predictive value of the systolic digital blood pressure. Part I: No arterial reconstruction.

The course of 53 patients presenting with 76 legs with ischaemia, defined by blood pressure on the big toe below 30 mmHg, were investigated. The series consists of those patients who after an angiography were considered non-candidates for arterial reconstructive surgery. There were 38 limbs with low pressure and severe ischaemic symptoms, i.e. rest pain and/or gangrene and 38 limbs with low pressures and only intermittent claudication or no leg symptoms at all. There were statistically significant differences in the fate of the limbs in the two groups, the first group suffering a 68 per cent risk of amputations compared to only 16 per cent in the second group. The corresponding risk of death was approximately 60 per cent and 10 per cent. The social fate was poor in the group with rest pain and/or gangrene due to the high number of amputations.

Adult↗
PubMed1989

The ischaemic leg: a long-term follow-up with special reference to the predictive value of the systolic digital blood pressure. Part II: After arterial reconstruction.

Ninety-five patients with 122 legs with a systolic blood pressure on the big toe below 30 mmHg were the subject of arterial reconstructive surgery. Investigating the limb survival rate we found the systolic blood pressure on the big toe to be the most important predictor, whereas there was found no predictive value of the ankle pressure/index. Preoperative clinical symptoms, which are closely related to the digital blood pressure, were significant predictors of the limb survival rate: the low-pressure limb with no symptoms or intermittent claudication had a significantly higher survival rate than the limb with rest pain and/or gangrene. Following arterial reconstructive surgery the social fate of patients with threatened legs was fairly good as 83% became fully independent.

Adult↗
PubMed1987

Catamenial pneumothorax.

Recurrent spontaneous pneumothorax associated with the menstrual bleeding--catamenial pneumothorax--is a rare condition. Only 56 cases have so far been described. Two cases are hereby presented, and the different ways of treatment are mentioned. The basic cause of the condition remains unknown.

Adult↗
PubMed1987

Search for circulating immune complexes and activation of the complement system in relation to estrogen treatment.

By employing an ammonium sulphate precipitation technique, previous studies have shown circulating immune complexes (cIC) in an increased proportion of women using oral contraceptives, as compared with non-users. The pathogenicity of cIC is well established, as deposition in organs may lead to activation of the complement system and thereby inflammation and tissue destruction. The purpose of the present study was to determine whether estrogen treatment could induce the formation of cIC, as measured by more specific immune complex assays. To test for complement activation, plasma samples were analysed for the complement split product C3d. Thirty-one women treated with estrogen for menopausal hormone deficiency symptoms and 38 untreated controls were analysed for cIC. Using a solid-phase C1q-anti-IgG binding assay, the mean level of cIC in the estrogen-treated group did not differ significantly from that of the untreated control group (p greater than 0.05). In a solid-phase C1q-protein A binding assay a very small average increase in the cIC values for the estrogen-treated group in comparison with the untreated group was found, expressing a significant difference (p = 0.04). Moreover, the C3d levels for the estrogen-treated group did not differ from the untreated control group. Finally, no differences could be demonstrated in cIC and C3d levels between patients treated with synthetic estrogens and patients treated with natural estrogens.

Antigen-Antibody Complex↗