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H J Hagedorn

Publications and source records attributed to H J Hagedorn.

11 recordsLinked to original sources
PubMed1992

Bacteriological and serological study of pertussis in Abeokuta, Nigeria.

Charcoal horse blood agar is the medium of choice for isolation of Bordetella pertussis from patients with early whooping cough. Since sterile animal blood often is not available in developing countries, a field study in Nigeria was undertaken to evaluate donated human blood as supplement to charcoal agar. Out of 209 children with suspected early pertussis, 33 were culture-positive (isolation rate 16%). Out of 188 children studied serologically by enzyme immunoassay, 36 (19%) were seropositive. The satisfactory isolation rate of 16% shows that culturing for B. pertussis on charcoal human blood agar can be tried in countries, where there is no regular supply of bacteriological media with animal blood.

Agar↗
PubMed1991

[Syphilis].

Explore the source record for details and available documents.

Humans↗
PubMed1987

[Cryptosporidiosis in immunocompetent patients. Epidemiology and clinical picture].

Cryptosporidiosis species were demonstrated in stool of 1.9% of 1600 unselected patients with enteritis (practically evenly divided between children and adults). Further investigations revealed seven cases of enteritis among family members. In eight patients there was a double infection with Cryptosporidium plus another enteritis pathogen. The most frequent clinical symptoms were diarrhea, vomiting, cramp-like abdominal pain, fever and headache. The mean period of parasite excretion was 14 days. Spontaneous cure occurred in all patients. Since cryptosporidiosis is relatively common not only in those with lowered resistance but also those who are immune-competent, search for Cryptosporidium should be included in all tests for etiologically uncertain cases of enteritis.

Adolescent↗
PubMed1984

[Incidence and diagnostic significance of reactive syphilis antibodies in systemic lupus erythematodes and other immunologic diseases].

Serum samples of 78 patients with systemic lupus erythematosus, systemic sclerosis and other immunological diseases were tested for antibodies to syphilis. Reactive or weak reactive results were observed in 10% by means of the treponema pallidum hemagglutination (TPHA) test, in 27% by the FTA-Abs test, in 40% applying the IgM-FTA-Abs test, in 10% by the VDRL test and in 3% of the cases using the cardiolipin CF test. Only in 3 patients (4%) we found an antibody pattern characteristic of syphilitic patients (TPHA and FTA-Abs test simultaneously undoubtedly reactive). Neither the comparative qualitative and quantitative determination of antibodies to ANA, nDNS and ENA (extractable salinesoluble nuclear antigen) nor elimination of nDNS and ENA antibodies, or incubation of the treponemal test antigen with DNase lead to a conclusion whether the reactive results of the TPHA, FTA-Abs, and IgM-FTA-Abs tests specifically indicate a syphilitic infection. The low incidence of reactive syphilis tests in SLE and the presence of syphilitic antibodies in other immunological diseases limitate the significance for the criterium in the diagnosis of SLE.

Antibodies, Bacterial↗
PubMed1983

[Comparison of 4 methods (immunofluorescence of antinuclear antibodies, indirect hemagglutination, immunodiffusion, counterimmunoelectrophoresis) for the differentiation and identification of nuclear antigen].

The performance of four methods (immunofluorescence of antinucleic antibodies (ANA), indirect haemagglutination, immunodiffusion and counterimmunoelectrophoresis) in the detection and differentiation of the antibodies against extractable nucleic antigen (ENA) was analysed with the aid of sera from 197 patients suffering from inflammatory diseases. Counterimmunoelectrophoresis showed the highest sensitivity and specificity for the detection and differentiation of antibodies against ribonucleoproteins (RNP), nuclear glycoprotein (Sm) and antibodies, which occur in Sicca (Sjögren) syndrome (SS-B, Ha). This method, like immunodiffusion, is suitable for demonstrating the common immunological identity of the ENA-antibody sera and the reference sera. The proof of immunological identity using the counterimmunoelectrophoresis was performed with the newly developed test system (pool procedure). This method, as compared to the procedure of Kurata & Tan ((1976) Arthritis Rheum. 19, 574-580) has certain technical advantages, and gives better identification.

Antigens, Nuclear↗
PubMed1979

[Present-day serological diagnosis of syphilis: comparison of TPHA and FTA-ABS tests with classical flocculation and complement-fixation reaction (author's transl)].

Twelve laboratories in the north Rhine-Westphalia participated in comparing TPHA and FTA-ABS tests with the so-called classical syphilis reactions (VDRL test, cardiolipin and pallida complement-fixation reaction, as well as Meinicke clearing reaction II). TPHA and FTA-ABS tests proved to be superior to the conventional methods both as regards specificity and sensitivity. Present-day serological tests of syphilis can thus be limited to the TPHA test to exclude syphilis, the FTA-ABS test as a confirmatory reaction and the VDRL test to judge the need or effectiveness of antisyphilitic treatment. These reactions which supersede the conventional syphilis tests serve for rational, reliable and cheap serological diagnosis of syphilis.

Cardiolipins↗
PubMed1977

[The agar diffusion method round table discussion on the methods and indications for determining blood levels of aminoglycosides (author's transl)].

The present-day technique of determining the concentration of aminoglycosides in blood by agar diffusion is described including the apparatuses, set-up, and reliability tests. The results of comparative investigations showed a higher degree of conformity in Mueller-Hinton agar than in standard agar. Due to its accuracy the agar diffusion method is especially suited for the calculation of dosage recommendations and for the prediction of the maximal and minimal blood levels during treatment with aminoglycosides.

Agar↗
PubMedDate not supplied

Comparison of erythromycin ethylsuccinate and co-trimoxazole for treatment of pertussis.

Fifty-five ambulatory children with early culture-proven pertussis were treated for two weeks either with erythromycin ethylsuccinate (n = 28) (50-80 mg/kg/day in three doses during meals) or with co-trimoxazole (n = 27) (6-10 mg trimethoprim/kg/day in two doses after meals). After completion of treatment, all patients in the erythromycin group were culture-negative, while in the co-trimoxazole group one child was still culture-positive. In this case vomiting may have played a role. Both agents appear to be able to eradicate Bordetella pertussis from the nasopharynx of patients with early whooping cough.

Anti-Bacterial Agents↗