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H J Huidekoper

Publications and source records attributed to H J Huidekoper.

6 recordsLinked to original sources
PubMed1987

Changes in the broncho-alveolar lavage fluid in smokers and patients with chronic obstructive lung disease.

Direct contact of the cells normally present in the bronchial lumen, such as alveolar macrophages, with air pollutants (e.g. cigarette smoke) can lead to the activation of these cells. This activation is beneficial for the cleaning task these cells have in the bronchial tree, but also leads to the release of chemotactic substances, toxic oxygen radicals, enzymes and mediators responsible for bronchial obstruction. As a first step in these processes, an enhanced chemotactic activity can attract neutrophils to the bronchial lumen, where they help by cleaning the lungs from possible dangerous intruders, but can also cause damage to the normal lung architecture. In the present study concerned with the pathogenesis of chronic obstructive lung disease (COLD), broncho-alveolar lavage (BAL) was performed in 35 individuals, who could be divided on he basis of their history and lung function into normal/nonsmokers, normal/smokers, COLD-patients/nonsmokers and COLD-patients/smokers. Neutrophilic chemotactic activity was assayed using Boyden chambers where the patients' own neutrophils were tested. More neutrophils and more neutrophilic chemotactic activity were found in the BAL-fluid of the smokers and COLD-patients. A correlation was demonstrated between the amount of chemotactic activity released during the incubation of cells obtained by BAL and the airway resistance or the airway conductance. These data suggest an enhanced chemotactic activity as one of the initiating factors in the pathogenesis of chronic obstructive lung disease.

Bronchoalveolar Lavage Fluid↗
PubMed1987

Changes in alveolar macrophage enzyme content and activity in smokers and patients with chronic obstructive lung disease.

An influx of polymorphonuclear and mononuclear cells into the lungs of smokers and patients with chronic obstructive lung disease (COLD) is thought to be an important factor in the development of pulmonary emphysema. Next to the synthesis and release of toxic oxygen radicals and mediators, an enhanced production and activity of proteolytic enzymes could play an important role in the pathogenesis of emphysema. In the present study changes were investigated in the broncho-alveolar lavage (BAL) fluid and BAL-cells, especially in alveolar macrophages. Pulmonary lavages were performed in the middle lobe with sterile saline of 37 degrees C in individuals, who could be divided on the basis of their history and lung function into normal/nonsmokers, normal/smokers, COLD-patients/nonsmokers and COLD-patients/smokers. Alveolar macrophages obtained by BAL were stained for different lysosomal enzymes. Isolated BAL-fluid and BAL-cells were assayed for elastolytic activity. In alveolar macrophages of smoking COLD-patients significantly more beta-glucuronidase could be demonstrated. Elastolytic activity changed with smoking habits, suggesting an enhanced release of elastolytic enzymes. No correlation was found between elastolytic activity and the amount of polymorphonuclear cells in the BAL-fluid. From these results it may be concluded that enzymes from alveolar macrophages play a more important role in the pathogenesis of emphysema than those from polymorphonuclear cells.

Female↗
PubMed1985

Sleeve resection.

Between 1972 and 1982, 43 patients underwent sleeve resection for different types of bronchial tumors. A 5-year survival could be calculated for 23 patients (48%), which is in the range of simple lobectomy. In the patients with lymph-gland involvement, the 5-year survival was 40%, in those not radically resected, it was 25%. All of the latter received irradiation after resection. All patients who died 5 or more years after the first operation had second primary bronchial carcinoma. In 27 patients we were able to compare lung function before and after the operation. Only in 3 patients lung function tests after resection were worse than expected, due to greater asthmatic bronchial obstruction. Ventilation-perfusion scanning was also performed in 22 patients. Only 3 scans showed diminished perfusion, probably as a result of postoperative irradiation.

Adult↗