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Biomedical subjects

H J Ingerslev

Publications and source records attributed to H J Ingerslev.

43 records · Page 3Linked to original sources
PubMed1982

Chlamydia trachomatis in acute and chronic endometritis.

The present report describes microbial, serological, and histological findings in chlamydial endometritis. Irregular bleeding, frequently seen in salpingitis patients, suggests endometritis, being a common manifestation of that disease. Chlamydia trachomatis is known to be a principal etiological agent of acute salpingitis. Chlamydiae can be recovered from the uterine cavity of such patients. A dense plasma-cell infiltration is seen in patients in whose endometrial epithelial cells chlamydial inclusions are found. A significant antibody response may be demonstrated in such patients. In acute salpingitis, the presence of chlamydial endometritis supports evidence from experimental animal studies that indicate a canalicular spread of chlamydiae from the cervix to the fallopian tubes.

Adult↗
PubMed1980

Bromelin for liquefaction of cervical mucus in sperm antibody testing: its effect on spermagglutinating immunoglobulin G.

Incubation of cervical mucus with the proteolytic enzyme bromelin is known to result in complete liquefaction of the gel. This effect has been used in the detection spermagglutinating antibodies in cervical mucus. However, the well-known papain-like effect of bromelin on human immunoglobulin G (IgG) might be expected to lead to an underestimation of the sperm antibody activity in cervical mucus. This possibility was investigated by studying the effect of bromelin on known amounts of purified IgG from four spermagglutinating sera. Serologic testing of bromelin-treated samples of cervical mucus mixed with purified IgG revealed no effect on spermagglutinin titers, whereas a significant effect was observed on purified IgG alone. Inert proteins in cervical mucus seem to protect the antibodies against degradation, apparently by substrate competition. Liquefaction of cervical mucus by bromelin seems to be a better basis for the assessment of the sperm antibody content of the mucus than extraction procedures, which have been shown to give a deficient yield.

Antibodies↗
PubMed1980

Spermagglutinating antibodies and sperm penetration of cervical mucus from infertile women with spermagglutinating antibodies in serum.

Spermagglutinins were demonstrated by the tray agglutination technique in cervical mucus collected during presumably-ovulatory cycles in 8 women among 21 patients with spermagglutinating antibodies in serum treated for infertility. A "poor" sperm penetration test was recorded exclusively in women with spermagglutinins in cervical mucus, and the results of the sperm-cervical mucus contact test were significantly correlated to the spermagglutinin titers in cervical mucus. The incidence of spermagglutinating antibodies in cervical mucus from infertile women was estimated to be 2.2% on the basis of the results in the present study. However, an inhibiting effect on sperm penetration in cervical mucus by spermagglutinins is expected to occur in less than 1% of women from infertile couples. A decrease in spermagglutinin titers in cervical mucus observed during estrogen medication was significantly associated with improved sperm penetration in vitro. The latter results may indicate a new approach to the treatment of infertility due to the presence of spermagglutinating antibodies in cervical mucus.

Antibody Formation↗
PubMed1980

Clinical findings in infertile women with circulating antibodies against spermatozoa.

The association between clinical findings and the occurrence of spermagglutinating antibodies was investigated in serum from women in 326 consecutive infertile couples. No relationship was found between unexplained infertility and the occurrence of sperm antibodies as detected by the tray agglutination technique. The occurrence of pregnancy at follow-up was the same in women with and without circulating sperm antibodies. However, the cumulative probability of conception per year was significantly lower in women with spermagglutinating antibodies than in women without such activity (0.005 greater than 2 P greater than 0.001), and the results showed a positive correlation between the duration of infertility and the occurrence of sperm antibodies. The latter findings may either reflect a fertility-reducing effect of the sperm antibodies or represent a time-related increasing tendency to develop sperm antibodies secondarily to the infertility.

Abortion, Incomplete↗
PubMed1979

Spermagglutinating antibodies and beta-spermagglutinins in sera from infertile and fertile women.

Sera from five groups of women were investigated by the tray agglutination technique for the presence of spermagglutinins: (1) 326 women from infertile couples, (2) 51 women in early pregnancy, (3) 65 women in advanced pregnancy, (4) 41 oral contraceptive users, and (5) 109 presumably fertile, nonpregnant women. By absorbing spermagglutinating sera with a cell-free eluate from spermatozoa, agglutination disappeared when due to a high-molecular weight compound with beta-mobility in preparative zone electrophoresis (denoted beta-spermagglutinin), whereas activity due to sperm antibodies was unchanged. Spermagglutinating sera occurred with comparable frequency among women from infertile couples, women in advanced pregnancy, and oral contraceptive users. The absorption study revealed agglutinating antibodies in a significantly higher frequency and in higher titers among women of infertile couples than among fertile women, in whom beta-spermagglutinins were found almost exclusively. These results demonstrate the importance of discriminating between sperm antibodies and beta-spermagglutinins in studies of sperm antibodies as a cause of infertility in women.

Agglutinins↗
PubMed1979

Characterization of sperm agglutinins in sera from infertile women.

Sperm agglutinins detectable by a tray agglutination technique in sera from women from infertile couples were characterized. Preparative zone electrophoresis of seven head-to-head-agglutinating sera revealed two patterns. In two sera sperm agglutinins were obtained in the gamma-globulin fraction, whereas in five sera the agglutinating activity was detected in the beta-globulin fraction only. By immunoaffinity chromatography, IgM and IgG sperm antibodies were demonstrated in the former two sera and also in two tail-to-tail-agglutinating sera. The beta"sperm agglutinin was not found to share physicochemical or immunological characteristics with beta-lipoprotein in the present investigation by ultracentrifugation, by precipitation of beta-lipoprotein with heparin and MnCl2, by absorption of beta-lipoprotein with colloidal silic acid, or by affinity chromatography on an anti-beta-lipoprotein column. An apparent neutralizing effect of anti-beta-lipoprotein by simple addition of antiserum to sera containing the beta-sperm agglutinin was shown to be due to a reaction with sperm components released from the spermatozoa by which the antiserum had been absorbed before use. These sperm components were found to neutralize the activity of the beta-sperm agglutinin only, whereas no effect was observed in sera containing sperm antibodies. By Sephadex G-200 fractionation the beta-sperm agglutinin was shown to have a high molecular weight (MW greater than or equal to 600,000). Ultrafiltration and dialysis experiments gave no evidence of involvement of low-molecular compounds in head-to-head agglutination.

Agglutination Tests↗