Biomedical subjects

H J Jariwala

Publications and source records attributed to H J Jariwala.

3 recordsLinked to original sources

PubMed1980

Haptoglobin phenotypes in leprosy.

Serum haptoglobin phenotypes were studied in 80 patients with leprosy classified according to the criteria of Ridley and Jopling. The distribution of phenotypes was: 2-2, 65%; 2-1, 27.5%; 1-1, 1.25%; and 0-0, 6.25%. This distribution was not significantly different from the controls except for the phenotype 0-0 (p less than 0.02). Thus, although this genetic marker did not correlate with the occurrence of the variety of disease, it is possible that leprosy caused inhibition of haptoglobin synthesis and therefore an apparent increased frequency of the 0-0 phenotype. Evidence for such a secondary anhaptoglobinemia was available in one case.

Haptoglobins↗

PubMed1980

Lactate dehydrogenase zymograms of skin biopsies in patients with leprosy. A preliminary report.

LDH isoenzymes were studied in tissue extracts of 78 cases of leprosy. All 25 control tissues showed five LDH isoenzymes corresponding to those of human sera. All tissues from the leprosy cases showed five similar bands. Seventeen cases showed additional LDH isoenzymes (anomalous bands). In 12 cases there was a single extra band with an eF value of either 0.125 +/- 0.015 or 0.525 +/- 0.015, and five cases showed both these bands. Additional bands were observed only in cases positive for acid-fast microorganisms (17 of 27 cases), and their presence correlated well with bacterial load (as judged by the BI) and viable organisms (as judged by the MI). Four cases with a high BI and MI did not show anomalous bands, however. A plausible explanation for these bands is that they originate from viable M. leprae.

Biopsy↗

PubMed1979

Fluorescence microscopy for detection of M. leprae in tissue sections.

The fluorescence method was compared with the Fite-Faraco method for detecting acid-fast microorganisms in paraffin sections of cases of leprosy. Biopsies were obtained from 50 cases of leprosy covering all varieties and at varying stages of treatment. The fluorescence method was better than the Fite-Faraco method; 22 biopsies showing acid-fast organisms in fluorescence microscopy and 20 in the Fite-Faraco method. Its superiority was evidenced in two cases in which the organisms were very scanty. Fluorescence microscopy can also be used to determine the Bacterial Index and the Morphologic Index of organisms. The Morphologic Index, however, was one and a half times higher than that obtained by the Fite-Faraco technic. The ease and speed of fluorescence microscopy appear to be a great advantage.

Diagnosis, Differential↗