PubMed Health⌕ Search

Biomedical subjects

H J Kreutzer

Publications and source records attributed to H J Kreutzer.

10 recordsLinked to original sources
PubMed2003

[Bladder rupture caused by spontaneous perforation of an infected urachal cyst].

Anomalies of the fetal urachus are rare. Normally, the postnatal urachus presents as a fibrous band extending from the bladder to the umbilicus. Urachal cysts may occur in postnatal life. Spontaneous perforation of urachal cysts is a very rare condition, which clinically may not be distinguishable from other acute abdominal conditions. We report a case of a 63-year-old male with a history of recurrent urinary tract infections and a bladder rupture caused by a spontaneous perforation of an infected urachal cyst. The symptomatology showed abdominal rigidity and pain, a palpable mass in the lower abdomen, and hematuria. Laboratory findings showed leukocytosis and an increased CRP level. The bladder rupture was confirmed by cystography. Bacteriologic examination identified Proteus vulgaris, Corynebacterium species, and Klebsiella pneumoniae. Most of the published cases in the literature report about intraperitoneal perforation of infected urachal cysts. In the present case, we found a spontaneous perforation of an infected urachal cyst leading to an extraperitoneal bladder rupture with an extraperitoneal limitation of the infection. The definitive therapy was complete surgical excision including a cuff of the bladder, drainage, and systemic broad-spectrum and local application of antibiotics. The further course was uneventful.

Catheters, Indwelling↗
PubMed1994

Quality control of "one touch" II blood glucose meters used by nurses in clinical departments.

Most analyses of blood glucose concentrations in our hospital are performed bedside by nurses with One Touch II blood glucose meters. The laboratory is responsible for the test results and therefore a quality control system which includes the performance by the nurses, is necessary. Human serum, spiked sterilised horse blood and (spiked) human blood were tested with 39 One Touch II blood glucose meters for usefulness in extra-laboratory quality control schemes. Serum gave high imprecision (CV > 9%) and leaked through the strips, contaminating the meters. Horse blood gave unacceptable imprecision (CV = 6.7%) and human blood could not be used due to an unpredictable time course of the glucose values in the distributed samples. Addition of NaF increased the CV of the measurements by more than 2%. Only the One Touch control solutions (3 levels) resulted in satisfactory precision (CV = ca. 4%) and are therefore suitable for extra-laboratory quality control of precision. However, accuracy control has to be done within the laboratory with fresh human blood.

Animals↗
PubMed1989

The immunofluorometric TSH assay as first-line test for suspected thyroid dysfunction.

In this study of 103 patients with suspected thyroid dysfunction, the diagnostic value of a single basal immunofluorometric (IFMA) TSH measurement was evaluated and compared with the classical TRH test with RIA-TSH measurements, plasma TT4 concentrations and FT4I. A single basal TSH determination accurately predicted the TRH-stimulated TSH response, making the TRH test redundant in most patients. Because undetectable basal TSH did not always exclude a small rise in TSH, the TRH test could still be indicated in patients receiving thyroxine suppression therapy for thyroid cancer. Basal TSH differentiated accurately between euthyroidism and thyroid dysfunction, especially at the decision values of 0.20 and 4.0 mU/l, as proposed in this study. For diagnosis of clinical and subclinical hypo- and hyperthyroidism, additional measurement of TT4 and/or FT4I is necessary. A 2-yr follow-up of patients with subclinical thyroid dysfunction did not show progression to clinical disease. In some of the patients with subclinical hypothyroidism, substitution therapy with thyroxine had been started after initial testing. Indications for treatment of subclinical thyroid dysfunction are discussed.

Adolescent↗
PubMed1986

Analytical evaluation of four sensitive assays of thyrotropin, including effects of variations in patient sampling.

Four immunometric kits for thyrotropin with detection limits below 0.1 milli-int. unit/L were evaluated with respect to accuracy, precision, specificity, matrix effects, and high-dose "hook" effect. We also studied variations of values related to the patients' sex and age and time of the day and season when samples were collected. Correlation among the four methods was excellent, except for a few samples, and the interference in these samples could be abolished by adding mouse serum or "suppression medium." These phenomena can also be expected to occur in other immunometric assays involving monoclonal antibodies. With some modifications all tests are suitable for a clinical study of the usefulness of the thyrotropin assay as a primary test for thyroid function.

Adult↗
PubMed1983

Kinetic measurement of total amylase and isoamylase activities with a centrifugal analyzer.

We evaluated a new kinetic assay for alpha-amylase (Phadebas IsoAmylase Test), using modified starch as the substrate and a CentrifiChem 400 centrifugal analyzer system. We determined isoamylase activities by using a selective inhibitor. Results were compared with those obtained with the chromogenic Phadebas dyed-starch technique. The method for total amylase appeared to be rapid and precise (CV = 4%) and results correlated well with the chromogenic method. Samples with activities up to 3000 arb. units/L can be analyzed without dilution. Glucose and pyruvate interfere with the assay, but hemoglobin and bilirubin do not. Pancreatic (P) and salivary (S) isoamylase activity can be determined with acceptable precision (CVP = 8%, CVS = 10%) by an automated routine procedure with commercially available reagents.

Adult↗
PubMed1976

An immunological turbidimetric method for serum transferrin determination.

The described method for the turbidimetric determination of serum transferrin concentration has the following features: the ionic strength of the medium is very low, the antiserum used is purified by the manufacturer, 25 mul antiserum per determination is sufficient, excess antibody or antigen has no influence. We studied the relation between transferrin concentration and total iron binding capacity for human normal and variant transferrins. Furthermore, our results are compared with the radial immunodiffusion technique.

Humans↗
PubMed1976

Turbidimetric determination of haptoglobin.

A specific, accurate and simple determination of haptoglobin is described with an antiserum against the antigenic determinants of the three haptoglobin phenotypes 1-1, 2-1 and 2-2. Antihaptoglobin is the determining factor in the formation of an antigen-antibody complex with maximal light absorption properties. Haptoglobin concentration is expressed as hemoglobin binding capacity. For the three haptoglobin phenotypes we found similar correlation between the turbidimetric method and the direct measurement of hemoglobin binding capacity by agar-electrophoresis.

Electrophoresis, Agar Gel↗