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Biomedical subjects

H J Wenisch

Publications and source records attributed to H J Wenisch.

At least 19 recordsLinked to original sources
PubMed1992

In vivo effects of TSH receptor antibodies in xenotransplanted human thyroid tissue.

From the data presented it can be concluded that the xenotransplantation model is well suited for studies concerning pathogenesis, diagnosis and therapy of AITD. Human thyroid transplants of Graves' disease tissue, depending upon extrinsic stimulators, lose all signs of hyperfunction after transplantation into athymic nude mice, but hyperfunction can again be induced in the transplants by injections of sera or immunoglobulin fractions from patients with active Graves' disease, containing stimulating TSH receptor antibodies. Our data demonstrate that thyroid function and growth are independent phenomena and can be differentiated by means of the xenotransplantation model. Furthermore the presented results indicate that TSH receptor antibodies are able to alter the expression of gene products in human thyroid transplants of Graves' disease, emphasising their important role in inducing/perpetuating immune phenomena related to AITD.

Animals↗
PubMed1992

[Multi-visceral upper abdominal resection and orthotopic liver transplantation--a surgical treatment concept for regionally metastatic tumors of the endocrine pancreas].

Islet cell carcinoma is rare. Even in advanced metastasizing tumors, relatively long survival times were reported. Aim of surgical treatment is complete removal of the tumor mass, if possible. When not, palliative procedures were described to be efficient in some of the cases. In the literature, among the first patients with regional metastasizing intraabdominal malignancies and upper abdominal exenteration, some cases with neuroendocrine tumors were reported. Aim of the following study is to describe the results in 4 patients with metastasizing islet cell carcinomas, which were treated by multi-visceral upper abdominal exenteration and combined orthotopic liver transplantation.

Adenoma, Islet Cell↗
PubMed1991

A rapid non-radioactive fluorescence assay for the measurement of both cell number and proliferation.

Measuring the incorporation of radioactive thymidine into the cell nucleus gives important information as to cell activation and proliferation. In this study the DNA-intercalating fluorochromes, Hoechst 33342 and Hoechst 33258, were tested as an alternative to the classical [3H]thymidine assay. Mitogen and alloantigen stimulated lymphocytes as well as FK 506 and CsA inhibited lymphocytes were treated with the two dyes, and the cell number and proliferation rates by means of measured fluorescence values. Of these tested fluorochromes H33342 appears to be an appropriate alternative to the [3H]thymidine assay. It mirrors the cell number in a fast and convenient manner without any pretreatment of the cell suspension which can remain in the culture plates. The complete assay procedure including data analysis can be performed rapidly and the standard deviations are small. This dye may also prove to be of value in other assay procedures, e.g., adhesion experiments.

Benzimidazoles↗
PubMed1991

[Diagnosis and therapy of malignant thyroid diseases].

The clinically important malignancies are characterized on the basis of 257 primary surgical procedures performed between 1973 and 1990 (9.1% of all thyroid operations during this period). Our own proceeding is characterized both in diagnostics and surgical technique in primary and recidive operations under special regard of a modified lymph node dissection. Obligatory and facultative adjuvant therapies are described. The follow-up schedule, which has found to be essential for the individual prognosis, should be discussed.

Chemotherapy, Adjuvant↗
PubMed1989

[Cellular immunologic parameters in thyroid diseases].

Between October 1984 and June 1987 in 45 patients (9 males, 36 females) pre- and postoperatively the immunocompetent cells in the peripheral blood were measured using OKT- and LEU-11-antigens. 20 patients suffered from malignant and 25 patients from benign diseases. For statistical analysis, H-test according to Kruskal and Wallis was used. Preoperatively, the total account of T-lymphocytes was decreased in patients with thyroid carcinomas, but showed no differences compound to patients with benign diseases postoperatively. No differences were observed in lymphocytic subpopulations in all groups. Parallely, in 50 patients (14 males, 36 females) immunocompetent cells were counted in the resected specimens. There were investigated 29 carcinomas and 21 benign thyroid lesions using the LEU-antigens. Between the group with malignant diseases and that with benigne lesions of the thyroid, no statistically significant differences were observed.

Adenocarcinoma↗
PubMed1988

[Leiomyosarcoma of the pulmonary artery--report of 2 cases from clinic practice and review of the literature].

Sarcoma of the pulmonary artery is a rare tumor. Only 81 cases are reported in the international literature. Leiomyosarcoma of the pulmonary artery is seen in 17% of these tumors. Most of them are not recognized during life but accidentally found at autopsy. So primary leiomyosarcoma of the pulmonary artery is a very rare tumor. We report about two new clinical cases and give a review of the literature.

Adult↗
PubMed1987

Evidence of in vivo iodine-induced hyperthyroidism in hyperfunctional autoimmune and autonomous human thyroid tissue xenotransplanted to nude mice.

The effect of different doses of continuous iodine infusion on xenotransplanted human thyroid tissue from toxic adenoma, and Graves' disease was examined using 131I scintigraphy in athymic nude mice. In spite of pretreatment with high iodine doses (1.25 micrograms or 12.5 micrograms 131I per day via i.p. implanted minipumps, Alzet 2002), the radioactivity localized in the transplanted tissue of toxic adenoma was more than 50% of the radioactivity in the transplants of the controls without iodine pretreatment 2 h after 131I injection, which was not a significant difference. Moreover, after high iodine treatment the 131I turnover rate in the thyroid transplants of toxic adenoma increased significantly. A tendency to an increased turnover rate was already observed with the lower dose. In contrast to that the transplants of Graves' disease tissue and mouse thyroids responded to high iodine treatment with a significant decrease in 131I retention. Serum of an untreated patient with active Graves' disease or injections of TSH increased 131I retention and the 131I turnover rate in the transplanted tissue of Graves' disease significantly (P less than 0.01). Iodine turnover was still increased after high iodine treatment. These results again show that thyroid tissue of toxic adenoma remains hyperfunctional after transplantation to athymic nude mice in contrast to thyroid tissue of Graves' disease which loses all signs of hyperfunction, when no exogenous stimulator is administered. In addition, these data clearly demonstrate, for the first time under in vivo conditions, that high iodine doses accelerate iodine turnover and thus presumably hyperfunction of human toxic adenoma in a dose-dependent manner as well as of the activated thyroid in Graves' disease.

Animals↗
PubMed1985

[Therapy of postoperative ileus (including peritonitis)].

In each case of postoperative ileus initial decompression of the intestine by an endoscopically placed tube is indicated (success rate 80%). With clear mechanical obstruction or diffuse peritonitis immediate relaparotomy is indicated. With prolonged gastrointestinal dilatation or paralytic ileus conservative therapy (tube-decompression, substitution of fluids and electrolytes, peristaltics, sympathicolysis with following stimulation) and close observation by an experienced surgeon is recommended. With local peritonitis (intraabdominal abscess) percutaneous puncture and drainage, sometimes just as a preliminary emergency procedure seems worthwhile.

Abdomen↗