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H J Winter

Publications and source records attributed to H J Winter.

8 recordsLinked to original sources
PubMed1995

Cytogenetic mapping of lambda gt10 lacZ sequences in the transgenic mouse strain 40.6 (Muta Mouse).

The transgenic mouse strain 40.6 (Muta Mouse) was developed for the detection of gene mutations induced in vivo. Strain 40.6 was constructed by microinjecting the shuttle vector lambda gt10 lacZ into the male pronucleus of a single cell embryo resulting from a CD2 (i.e. BALB/c x DBA/2)F1 x CD2F1 cross. Approximately 40 concatenated copies of the shuttle vector were integrated per haploid genome. The resulting mice were bred to disomy for the insert for use in mutagenicity studies. Ultimately, it is hoped that transgenic rodent model systems such as this one will play an important regulatory role in hazard identification. Despite the increasing use of this strain in toxicological studies, relatively little is known about the site of integration of the target gene into the mouse genome. In this study, fluorescence in situ hybridization and DAPI chromosome banding were combined to determine the location of the transgenic element in the mouse genome. The results indicate that the lambda sequences containing the lacZ gene are located in the B region of mouse chromosome 3. No other major chromosomal rearrangements were evident in the genome of this mouse strain.

Animals↗
PubMed1988

Faecal incontinence in diabetes mellitus: is it correlated to diabetic autonomic or peripheral neuropathy?

Faecal incontinence is a frequent manifestation of diabetic enteropathy. The purpose of this study was to determine whether faecal incontinence in diabetes mellitus correlates with manifestations of diabetic autonomic or peripheral neuropathy at other organ sites. In 12 incontinent and 15 continent diabetics stool frequency and stool continence, basal and squeeze anal sphincter pressures, and continence to rectally infused isotonic saline solution (1500 ml) were prospectively evaluated. These data were correlated to quantitative measures of autonomic neuropathy as assessed by heart rate variation and pupillary reflex response to light, and to quantitative measures of peripheral neuropathy as assessed by nerve conduction velocity and sensitivity to vibration. Incontinent diabetics exhibited decreased basal and squeeze anal sphincter pressures, and reduced continence for fluid compared to their continent controls. The degree of incontinence correlated well with the maximal volume of retained rectally infused saline solution, but neither with basal and squeeze anal sphincter pressures, nor with the severity of autonomic or peripheral neuropathy at other organ sites. It is concluded that a generalized dysfunction of the autonomic or peripheral nervous system does not play a major role in the pathogenesis of faecal incontinence in diabetes mellitus. The great overlap of basal and squeeze anal sphincter pressures in incontinent and continent diabetics raise evidence for disturbances of additional extrasphincteric factors as part of the pathomechanism of faecal incontinence in diabetes mellitus.

Adolescent↗
PubMed1982

[The dorsal metacarpal ligament I and arthrosis of the saddle joint of the thumb].

Morphology and function of the Ligamentum metacarpeum dorsale I are investigated. The ligament connects the capsule of the Articulatio carpometacarpea pollicis with the base of the second metacarpal bone. The ligament is responsible for the security and passive guidance of movements in the saddle joint of the thumb. In the course of an arthrotic affection of the Articulatio carpometacarpea pollicis the ligament is exposed to increased tensile stresses. The excessive mechanical stress often leads to partial ossification of the ligament. The ossification is radiologically identified as a spurlike protrusion at the base of the second metacarpal bone. The evaluation of clinical X-rays reveals, that an osseous spur can develop before the saddle joint of the thumb shows alterations. From this the bony spur is important for an early diagnosis of the rhizarthrosis.

Aged↗
PubMedDate not supplied

[The significance of bone spurs at the base of the 2d metacarpal bone in the pathogenesis of joint arthrosis of the base of the thumb].

A causal correlation is proved between an outgrowing osseous spur at the Os metacarpale II and the rhizarthrosis. The midhand bone of the thumb is connected by a ligament with the base of the Os metacarpale II. High tensile stresses elongate the ligament and lead to its partial ossification. The ligament looses its essential function and the thumb subluxates radially.

Exostoses↗