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Biomedical subjects

H Jia

Publications and source records attributed to H Jia.

At least 37 records · Page 2Linked to original sources
PubMed2001

[A successive study of histopathological changes in unilateral facial muscle denervation].

OBJECTIVE: To observe the histopathological changes in unilateral facial muscle denervation. METHODS: Gomori trichrome stain, acridine orange (AO) fluorescence, enzyme-histochemistry and immunohistochemistry techniques were used for studying mitochondria, nuclei acid concentration, enzymatic activity and contraction protein expression of facial muscle in different denervation period. RESULTS: Mitochondrian function, enzymatic activity and contraction protein expression of facial muscle denervation increased in the first two weeks, then declined till 6 months. Myofiber's histotype began to transform in 1 month after denervation. Type-grouping was obvious in 6 month. Regeneration myofibers were also seen at this time. CONCLUSIONS: Unilateral facial muscles become atrophy after denervation. They have proliferating tendency from 2 month to 6 month. Therefore, within 1 month after denervation measures to benefit atrophy will be more effective.

Animals↗
PubMed2001

[T cell and molecule-1 detection in cornea of fetus, neonates, children and adults].

OBJECTIVE: To study the immune state of human cornea with different ages. METHODS: The human corneas in different ages were detected by using immunohistochemical techniques for 5 types of monoclonal antibody. RESULTS: The contents of T-cell (CD(+)(3)), helper/inducer (CD(+)(4)), toxic T-cell (CD(+)(8)), macrophage (CD(+)(68)) and molecule-1 (intercellular adhesive molecule, CD(+)(54)) in the adult cornea were relatively low, mainly in the limbus but none at the corneal center. These positive cells were less in corneas of fetus, neonates and children than that in adults. CONCLUSION: In corneas of fetus, neonates and children, the T-cell, macrophage and molecule-1 were less than that of adults. Their corneas may be used as donors in corneal transplantation.

Adult↗
PubMed2001

[Raman analysis of SiGe films grown by UHV/CVD].

This paper presents a Raman method, which is no deconstructed, to measure Ge content and strain in SiGe alloy films and some samples, which is grown by UHV/CVD with different Ge content and thickness, have been tested by above method. Two samples among them were measured by DCXRD, the results tested by DCXRD are consistent with Raman results that proves that the method in this paper is reliable and accurate. The SiGe PHMOSFET has been fabricated by the samples, the transconductance reaches 112 ms.mm-1 for the device with 0.5 micron channel.

Alloys↗
PubMed2000

Characterization and transcriptional profiles of two rice MADS-box genes.

The plant MADS-box gene family plays a key role in plant development, especially in flower development. We designed degenerate primer according to the MADS-box conserved region and isolated two cDNA from rice, FDRMADS6 and FDRMADS7, which are homologous to AP1. RT-PCR expression analyses by using total RNA isolated from root, shoot and flower showed that the FDRMADS6 transcript was detectable only in flower while FDRMADS7 was expressed in all three tissues. In situ hybridization experiments indicated that at the early stage of rice flower development, the transcripts of FDRMADS6 and FDRMADS7 were detected in the spikelet apical meristem, which were same as AP1. At the late stage, when flower organ primordia started differentiating, the expression of FDRMADS6 appeared to be specifically localized in developing stamens and the pistil primordia, while the transcripts of FDRMADS7 were detectable abundantly throughout the organ primordia. Our results suggest the two MADS-box genes may be members of the AP1 family, but may have different functions.

Journal Article↗
PubMed2000

beta2-adrenoceptor gene polymorphisms and blood pressure variations in East Anglian Caucasians.

OBJECTIVE: The amino-terminal polymorphisms, Arg16Gly and Gln27Glu, of the beta2-adrenergic receptor (beta2AR) have been shown to affect regulation of the receptor expression by an agonist in cell culture studies. The Arg16Gly polymorphism has also been recently shown to be associated with essential hypertension. We therefore evaluated whether the amino-terminal polymorphisms of beta2AR are associated with hypertension in a Caucasian population. SUBJECTS AND METHODS: We performed an association study in 298 hypertensive patients and an equal number of age-matched normotensive controls from the East Anglian region, with blood pressure assessed categorically and quantitatively. We also examined the influence of the amino-terminal polymorphisms on blood pressure response to beta-blockade in 144 of the patients randomly assigned to this class of drug. Genotyping of the Arg16Gly polymorphism was undertaken by a newly designed mismatched polymerase chain reaction (PCR) and digestion with Nde I, whereas the Gln27Glu polymorphism was genotyped by PCR followed by Fnu4H I cleavage. RESULTS: We found no differences in the genotype or allele frequencies of the beta2AR polymorphisms between hypertensive and normotensive participants. There was also no association between the beta2AR genotypes and variations in either basal blood pressure or the blood pressure response to a beta-blocker. CONCLUSION: These findings suggest that the amino-terminal polymorphisms of the beta2AR gene are unlikely to constitute major susceptibility for essential hypertension in the East Anglian population.

Adrenergic beta-Antagonists↗
PubMed2000

What determines severity among patients with painful functional bowel disorders?

OBJECTIVE: For patients with painful functional bowel disorders (FBD), physicians frequently make diagnostic and treatment decisions based on the severity of the pain reported; patients with severe painful complaints may receive extensive diagnostic tests and treatments. Therefore, it would be important to determine what clinical factors contribute to the judgment of severity among patients with FBD. The aim of this study was to identify the psychosocial, behavioral, and physiological (visceral sensitivity) factors that predicted severity in patients with moderate to severe FBD. METHODS: Two hundred eleven female patients with moderate or severe FBD, as determined by the Functional Bowel Disorder Severity Index, entered a multicenter treatment trial at the University of North Carolina and the University of Toronto. Patients filled out diary cards and were given questionnaires and physiological testing (rectal sensitivity using barostat). Analysis of covariance and logistic regression adjusting for demographic factors were performed to determine which factors distinguished patients at study entry with moderate from those with severe FBD. RESULTS: Patients with severe FBD were characterized by greater depression and psychological distress, poorer physical functioning and health-related quality of life, more maladaptive coping strategies, and greater health care utilization. There was a trend for patients with severe FBD to have lower rectal sensation thresholds. Regression analysis indicated that severity was best predicted by behavioral features: poorer daily physical function, difficulties related to eating, more phone calls to the physician, and more days in bed for GI symptoms. CONCLUSIONS: We conclude that patient illness behaviors are best correlated with severity in FBD. The use of psychopharmacological agents (e.g., antidepressants) and psychological treatments to treat psychiatric comorbidity and to improve behavioral coping styles is recommended. Training to help medical physicians identify and respond to psychosocial and behavioral features of these conditions is likely to improve patient satisfaction with their care and the clinical outcome.

Adolescent↗
PubMed2000

Further validation of the IBS-QOL: a disease-specific quality-of-life questionnaire.

OBJECTIVE: There has been growing interest in the investigation of health-related quality of life (HRQOL) among patients with gastrointestinal (GI) disorders. We recently reported on the development and preliminary validation of the IBS-QOL, a specific quality-of-life measure for irritable bowel syndrome (IBS). The aim of this study was to determine the longitudinal construct validity (responsiveness) of the IBS-QOL. METHODS: Female patients enrolled in a multicenter treatment trial for functional bowel disorders were studied pre- and posttreatment with the IBS-QOL and other health status measures. Based on the response to treatment for several variables (pain/14-day score, daily function, and days in bed/3 months), patients were stratified into Responders, Partial Responders, and Nonresponders. Change scores in the IBS-QOL were then statistically compared with changes in the other variables to determine their correlation and whether Responders were significantly different from non- and Partial Responders on the IBS-QOL. RESULTS: There was a significant correlation between change scores on the IBS-QOL and the other measures of treatment effect (Pain/14 days, r = 0.25, p < 0.002; Sickness Impact Profile [SIP] Total Score, r = 0.28, p < 0.0004). In addition, the IBS-QOL scores significantly differentiated Responders from Nonresponders for most of the variables tested (regression trend test for Pain/14 days, p < 0.04; SIP Total, p < 0.0001; SIP Physical, p < 0.0001; SIP Psychosocial, p < 0.002, and SIP Eating, p < 0.04). CONCLUSION: The IBS-QOL is responsive to treatment in a referral-based clinical population of patients with functional bowel disorders.

Adult↗
PubMed2000

A genome-wide search for susceptibility loci to human essential hypertension.

We undertook a systematic search of the entire human genome with the affected sibling-pair model to identify major susceptibility loci to essential hypertension. Affected nuclear families (n=263) were recruited and divided according to definite or probable genetic contribution to hypertension depending on number of hypertensive siblings. The largest nuclear families were first screened with a set of microsatellite markers. Regions on the genome with P<0.05 were tested against the second set of smaller families. An exclusion map was generated to identify regions in which hypertension-causing genes are unlikely to reside. Sibling-pair linkage analysis identified a single locus on chromosome 11q (P<0.004) in the first pass. A second pass with nuclear families that had only affected sibling pairs was, as expected, insufficient to support linkage to 11q. Multipoint exclusion-linkage analysis showed that 3 genetic loci are necessary to explain familial aggregation of essential hypertension. Our preliminary findings suggest that no single region within the human genome contains genes with a major contribution to essential hypertension. We show that the disease is indeed polygenic, with each gene providing a relatively small risk. Our exclusion map will help future investigators to concentrate on areas likely to contain these genes. The region on chromosome 11 is the first to point to a new candidate gene for hypertension that has arisen out of a genome search, but replication of these results at a higher significance is necessary before positional cloning can be justified.

Aged↗
PubMed2000

A clinical investigation on garlicin injectio for treatment of unstable angina pectoris and its actions on plasma endothelin and blood sugar levels.

To investigate the therapeutic effects and mechanisms of garlicin for treatment of unstable angina pectoris (UAP), garlicin injectio was intravenously dripped 60 mg/day in 34 cases for 10 days. Nitroglycerine was used in 21 cases of the control group. The results showed that the total effective rates in improving symptoms and electrocardiogram after garlicin treatment were respectively 82% and 62%, and that the plasma endothelin and blood sugar levels were markedly lowered in cases with hyperglycemia.

Aged↗
PubMed2000

The expression kinetics of myogenin in facial muscle denervation.

OBJECTIVE: To study the expression kinetics of myogenin in long-term denervated facial muscle and to explore the possibility of gene therapy for facial muscle paralysis with myogenin gene. MATERIALS AND METHODS: In 48 New Zealand rabbits, buccal muscle paralysis of one side was produced by excision of 1 cm segment buccal branch of the facial nerve. The opposite side served as controls. The animals were sacrificed and the buccal muscles of both sides were removed for examination at 1 day, 3 days, 1 week, 2 weeks, 1 month, 2 months, 4 months, and 6 months after the initial operation. The myogenin expression in denervated and innervated buccal muscles was analyzed by Western blot. Satellite cell proliferation was detected with proliferating cell nuclear antigen (PCNA) analysis. Muscle nucleic acid concentration was determined through acridine orange (AO) staining method. RESULTS: Myogenin expression increased to the highest level at 3 days after denervation, thereafter it gradually decreased. The Western blotting signal for myogenin intensified again after 1 month and at 4 months. In contrast, the highest expression of the controls (innervated muscles) were observed at 1 month. These changes in myogenin expression in denervated buccal muscles were consistent with the change in satellite cell proliferation and in muscle nucleic acid concentration. CONCLUSION: Myogenin protein expression in longterm facial muscle denervation is closely associated with satellite cell regeneration. Myogenin may promote satellite cell differentiation, and therefore may improve the treatment of facial paralysis.

Acridine Orange↗
PubMed2000

[Preliminary study on purification function of reed wetland for nutrients from land sources].

The preliminary study on purification function of reed wetland for nutrients from land sources were conducted by irrigation channels and lysimeters. The results show that the purification rates for N, P and CODcr are from 41.7%-64.71% in the channels and 60.0%-75.92% in the lysimeters. The contents of N and P reached the national second level standards of sea water after filtration by lysimeters. By harvest, the good recycle between wetland and land is formed without accumulation.

Ecology↗
PubMed2000

Effect of prenatal tetrandrine therapy on pulmonary vascular structural remodeling in the nitrofen-induced CDH rat model.

OBJECTIVE: To examine the effects of prenatal tetrandrine (Tet) therapy on pulmonary arterial structural remodeling in nitrofen-induced congenital diaphragmatic hernia (CDH). METHODS: CDH was induced in fetal rats by maternal administration of 100 mg nitrofen by gavage on day 9.5 of gestation (term, day 22). Control animals received olive oil (OO). Tet (24 mg/kg per day) or normal saline (NS) was given by gavage every day from 16 to 20 days of gestation, and fetuses were delivered by caesarean section on day 21.5. Lung sections from 3 fetuses in each group were studied. The number of vessels were calculated, the external diameter (ED), medial wall thickness (MT), percent of medial wall thickness, and wall structure were evaluated by image analysis software. RESULTS: In the pre-acinar arteries, CDH-NS pups had a significantly increased %MT compared with the OO-NS group (P < 0.05), while CDH-Tet animals had a reduced %MT compared with the CDH-NS rats (P < 0.05). Similar results were seen in the intra-acinar level. Significant differences were observed between CDH-NS animals and OO-NS controls in the percentage of muscularized intra-acinar blood vessels (P < 0.001). Tet-treated CDH pups had a reduced percentage of muscularized intra-acinar arteries compared with CDH-NS animals. CONCLUSIONS: Medial hypertrophy is present in both the pre-acinar and intra-acinar arteries in the nitrofen-induced CDH rat model. Tet treatment inhibits medial hypertrophy and reduces the percentage of muscularized intra-acinar vessels. Prenatal Tet therapy may be efficacious in reducing the risk of PH in human newborns with CDH.

Alkaloids↗
PubMed2000

[Effect on keratocyte-mediated collagen degradation by Pseudomonas aeruginosa].

OBJECTIVE: To study the pathogenesis of cornea melting (ulceration) by pseudomona (P) aeruginosa for instruction of clinical treatment. METHODS: Type I collagen gels with or without suspended keratocytes were incubated for 24 hours under medium containing sterile P. aeruginosa culture broth. Native collagen fibrils were removed from the media by ultrafiltration. The ultrafiltrates were then hydrolyzed, and the amount of hydroxyproline was measured spectrophotometrically. The effect of a synthetic matrix metalloproteinase (MMP) inhibitor, Galardin, on collagen degradation was also examined. RESULTS: P. aeruginosa broth induced type I collagen gel degradation directly. In the presence of keratocytes, degradation by P. aeruginosa broth was enhanced. Galardin significantly reduced the amount of collagen degraded by P. aeruginosa culture broth, no matter keratocytes were present or not. CONCLUSION: P. aeruginosa culture broth directly degrades type I collagen and also increases keratocyte-mediated collagen degradation. The result is helpful to the clinical treatment of cornea melting caused by P. aeruginosa, and the mechanism should be further studied.

Animals↗
PubMed2000

[3D finite element analysis of stress distributions in supporting tissues of clasp-type partial dentures of transferring occlusion force].

OBJECTIVE: To provide a theoretical basis for clinical application of the clasp-type partial denture of transferring occlusion force, the stress distributions in its supporting tissue were analyzed. METHODS: The 3D finite element model was made, and the features of stress distributions in the supporting tissues of the clasp-type partial dentures which had different rest recess angles were discussed. RESULTS: The medial stress component was significantly larger than the distal one in the supporting tissue around main abutment root. When the rest recess angles were equal or greater to 60 degrees, the stress values were greatly reduced in the supporting tissue around the roots of the three abutments. When the rest recess angle reached 75 degrees, the stress values in the supporting tissue around the main abutment root wore decreased by 30% than the angle 0 degree and by 52% than the angle 45 degrees, the stress values in the supporting tissue around the root of second abutment (adjacent to the main abutment) were wholly increased by 44% than the angle 0 degree and reduced by 23% than the angle 45 degrees, the stress values in supporting tissue around the root of third abutment (outside of the second abutment) were wholly increased by 53% than the angle 0 degree and decreased by 31% than the angle 45 degrees, the stress values in the medial root of the main abutment were reduced by 73%. CONCLUSION: The clasp-type partial denture can resolve part of the occlusion force into two components the medial and distal components, and the occlusion force is distributed to several abutments, at the same time the conversed force components can be canceled by each other. So the whole stress values are greatly reduced and the stress around abutment root is greatly decreased. As a result the damage to the abutment will be eliminated. A design with centralized multi abutments and multi fixed bodies are used in this denture, which are more fixed and stable and have no obvious sinkage in the plate base. Therefore, the mastication function can be satisfactorily recovered, and the reasonable rest recess angle for the clasp-type partial denture of transferring occlusion force is about 75 degrees.

Bite Force↗
PubMed2000

[The structure of DL-homocysteic acid in D2O solution].

DL-Homocysteic acid (DLH) is an important free amino acid in central nervous system of human body. In this paper the structure of DLH in D2O solution was discussed. The FTIR and FT-Raman spectra of DLH, which were obtained by Nicolet Magna-IR 750 II FTIR spectrometer and 950 FT-Raman spectrometer, were compared between its solid state and its D2O solution. The results indicated that the H-bonding structure of DLH in solid state was rearranged in D2O solution. The appearance of 1,620 and 1,408 cm-1 suggests the partly ionization of -COOH. From above, we can conclude that a new H-bonding structure of DLH was formed in D2O solution. The H-bond of C-O...H-N was replaced by C-O...D-O, and the H-bonds of -NH3+ and -SO3- were also varied by D2O molecular. Furthermore, the peak shift and the intensity changes of -CH2 in FTIR and FT-Raman spectra were also proved that the skeleton structure of DLH was changed in D2O solution. In addition, this new structure did not alter with the change of concentration of DLH.

Deuterium Oxide↗
PubMed2000

[Spectroscopic study on DL-homocysteic acid and it's complexes].

The main IR bands of the DL-Homocysteic acid was preliminary assigned. The complexes of DL-Homocysteic acid with Na+, K+, Ca2+ were synthesized. Element analysis and TGA-DTA were used to conclude the compositions of these complexes. The FTIR and FT-Raman spectra were studied to distinguish the different coordination structures. The bands of carboxyl, sulfonate and amino groups are all shifted. The peaks of-CH2- also changed a lot. The results indicated that the H-bonding structures among the moleculars were rearranged. The carboxyl and sulfonate groups of DLH both coordinated with the metal ions. And the coordination structure of C=O group in the three complexes were all monodentated.

Calcium↗
PubMed1999

Isolation and mapping of rFUS6, a rice orthologue of Arabidopsis thaliana FUS6.

COP9 complex is one of the most important components that act in repressing photomorphogenesis in Arabidopsis thaliana. FUS6 has been identified as one of eight subunits of the COP9 complex in Arabidopsis. Using Arabidopsis Fus6 cDNA as a probe, we screened a rice root cDNA library and a rice genomic library. A 1730-bp cDNA was obtained, which has an open reading frame corresponding to 441-amino-acid. This 441 amino acids putative protein has 67% identity with Arabidopsis COP11/FUS6 (AtFUS6) and 40% identity with human GPS1, an AtFUS6 orthologue. So we designated this novel gene as rFUS6. The 6.2-kb genomic sequence of rFUS6 was also obtained. Sequence comparison showed that the rFUS6 gene had six exons and five introns. Sequence inspection of the 5'-flanking region revealed the presence of some potential light-regulated cis-elements such as a G-box, GT-1 binding sites, and a TGACG motif. Southern hybridization with rice total DNA showed that rFUS6 was perhaps a single copy gene. The rFUS6 locus was mapped by hybridization with a rice BAC library membrane and the results showed that rFUS6 had a locus at 16.3 cM of chromosome 1.

Amino Acid Sequence↗