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Biomedical subjects

H K Andersen

Publications and source records attributed to H K Andersen.

78 records · Page 5Linked to original sources
PubMedDate not supplied

Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM.

IgM and IgG ELISA to Puumala virus were evaluated using sera from patients with haemorrhagic fever with renal syndrome (HFRS) from different geographical regions: Sweden, Denmark, Norway, Belgium and the European USSR. IgM ELISA proved useful in the diagnosis of HFRS in patients from all the regions mentioned above. Specific IgM could be detected as early as day 1 post onset of disease, and patients remained IgM-positive for several months. Specific IgG ELISA antibodies were also frequently detected in acute sera, and acute-convalescent serum pairs often failed to show a significant titre rise or increase in optical density (OD) values. This limits the use of IgG ELISA in patient diagnosis. Sera collected 2 years after infection revealed higher IgG ELISA OD readings than convalescent sera, and very high values were still detectable 10 to 20 years postinfection. IgG ELISA is therefore useful for the testing of immunity and in seroepidemiological studies. Acute and convalescent sera from HFRS patients in Korea and the Asian USSR showed no or only very weak reactivity in the Puumala virus IgG and IgM ELISA. These results are consistent with the "one-way" crossing described earlier.

Adolescent↗
PubMedDate not supplied

In vitro stimulation of human lymphocytes by purified cytomegalovirus.

Lymphocytes from 19 healthy donors were tested against purified cytomegalovirus (CMV) strain Ad 169, in a lymphocyte-transformation test. The test was performed in microcultures using various preparations and concentrations of antigen. These included, besides purified CMV, CMV-infected cells, herpes simplex virus type 1 antigen, and control antigens. Lymphocytes from CMV-seropositive donors were stimulated by purified virus and infected cells, whereas lymphocytes from seronegative donors did not respond. Similarly, only lymphocytes from herpes-seropositive donors did respond to herpes antigen. With these preparations of antigen the test was found to be sensitive and specific.

Antibodies, Viral↗
PubMedDate not supplied

High levels of complement fixing antibodies against cytomegalovirus in patients with primary Sjögren's syndrome.

Antibodies to cytomegalovirus (CMV) were examined in sera from 21 consecutive patients with primary Sjögren's syndrome (SS) and 19 consecutive patients with secondary SS, using a complement fixation (CF) test and an antibody capture enzyme-linked immunosorbent assay (ELISA). Sera from 15 CMV-negative subjects, 15 CMV-positive subjects, 3 patients with primary CMV infection and 3 patients with recurrent CMV infection served as controls. The prevalence of CMV antibodies in the patients with primary and secondary SS was found similar to the prevalence known to occur in the normal adult population. Unrelated to clinical parameters, 5 patients with primary SS (24%) had high levels of CF antibodies against CMV. Ig class antibodies to CMV were not elevated in these 5 patients. Preferential production of CF antibodies to CMV may be a pathogenetic factor in some patients with primary SS.

Adult↗