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H Köck

Publications and source records attributed to H Köck.

4 recordsLinked to original sources
PubMed2005

[Experiences and results with the locked compression plate for 603 fractures of the distal radius].

Between January 1999 and December 2003 we have treated 603 patients with a fracture of the distal radius without concomitant injuries with the Synthes locked compression plate. The locked compression plate, the concept of the postoperative treatment, and our results are presented. In most cases we have used the palmar plate. We see an indication for the palmar plate in the A2 and A3, B2 and B3 and in all C fractures. It is also practicable for fractures with dorsal comminutions. Because of its permanent retention due to the subchondral fixation of the splints (internal fixation), this new plate makes early mobilization possible. The palmar approach with its good soft-tissue coverage not only reduces the risk of infections but also offers the possibility of not having to remove the plate. A cancellous bone graft is only seldom necessary.

Adolescent↗
PubMed1996

Adenovirus-mediated p53 gene transfer suppresses growth of human glioblastoma cells in vitro and in vivo.

Alterations in the p53 tumor-suppressor gene occur in 35-60% of human glioblastomas, and re-introduction of p53 can suppress neoplastic growth. To evaluate the potential for p53 gene therapy of glioblastoma, we have analyzed the response of human glioblastoma cell lines in vitro and in vivo to experimental therapy with replication-deficient recombinant adenoviruses encoding wild-type p53 (rAd-p53). Western blot analyses showed high-level expression of p53 protein after treatment with rAd-p53, and transgene expression was dependent on promoter strength. A p53-specific dose-dependent inhibition of in vitro cellular proliferation was observed in 5 of 6 cell lines, and growth inhibition corresponded to adenovirus-mediated gene transfer and expression. p53-specific cell death was quantitated by release of the lactate dehydrogenase enzyme. Fragmentation of DNA into nucleosomal oligomers and the occurrence of a hypodiploid cell population detected by flow cytometry provided evidence for apoptosis. Studies in nude mice demonstrated that ex vivo infection with rAd-p53 suppressed the tumorigenic potential of human glioblastoma cells. Furthermore, direct injection of rAd-p53 into established s.c. xenografts inhibited tumor growth. Our observations suggest that re-introduction of wild-type p53 may have potential clinical utility for gene therapy of glioblastoma.

Adenoviridae↗