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Biomedical subjects

H Köhler

Publications and source records attributed to H Köhler.

At least 541 records · Page 30Linked to original sources
PubMed1975

[Cellular immunity to donor antigens after kidney transplants in humans].

Decisive for controlling renal transplantation is the knowledge of the immunological reactions. The cellular immuno-mechanisms, released via sensibilization by donor HL-A antigens, are specially important for acute rejection. To predict a crisis of rejection and to initiate increased immuno-suppressive measures it is important to know the degree of cellular immunologic response. The sensibilization was demonstrated in the experiments by means of the MEM test, using graft antigens isolated from spleen or kidney of the donor. In all patients an increasing sensibilization against donor antigen could be proved. The velocity of this sensibilization seems of value for predicting the duration of the graft's survival.

Electrophoresis↗
PubMed1975

The primary structure of a human lambda II chain.

The human myeloma protein Boh (gamma 2, lambda) was isolated and completely reduced and aminoethylated. The light chain was obtained by chromatography on Sephadex G-100 in 4 M guanidine HC1. The amino-terminal sequence on the blocked light chain could be determined by automatic sequence degradation after PCAase treatment. Twenty-one peptides were isolated from a tryptic digest and 12 peptides from a chymotryptic digest. The sequence determination on these peptides was performed by automatic sequencing methods. The light chain of Boh protein belongs to the lambda II subgroup. Unique substitutions have been found at position 8 (Arg) and position 62 (Tyr). Furthermore, the Boh light chain has six cysteine residues, the additional (sixth) cysteine being adjacent to the invariable intrachain-S-S linking cysteine at position 91. Sequence comparison of lambda II proteins reveals a high degree of homology emphasizing the biologic significance of the hypervariable region sequences;

Amino Acid Sequence↗
PubMed1974

Neonatal tolerance induced by antibody against antigen-specific receptor.

Specific immunologic unresponsiveness is induced by injecting adult or neonatal mice with antibody against antigen-specific receptor (antireceptor antibody). Suppression in mice treated as adults lasts several weeks, and cells from these suppressed mice respond normally in culture. In contrast, unresponsiveness induced in neonatal mice is long-lasting; cells from these mice do not respond in culture and do not affect the response of normal cells. Evidently, antireceptor antibody reversibly blocks antigen receptors in adult animals, but induces unresponsiveness in neonatal mice by depleting the clone of receptor-bearing cells.

Animals↗
PubMed1974

Clonal depletion in neonatal tolerance.

Specific unresponsiveness can be induced in neonatal and adult BALB/c mice by antibody against antigen-specific receptor (antireceptor antibody). When heterologous antireceptor antibody is used in the indirect fluorescence technique, the number of fluorescent cells in these animals is significantly lower than in normal animals. Fluorescent cells appear after a relatively brief incubation of cells from adult-suppressed animals, whereas no fluorescent cells are detected when cells from neonatally treated animals are incubated briefly. Evidently, treating neonatal mice with antireceptor antibody specifically depletes the antigen-responsive clone. In contrast, antireceptor antibody causes reversible blockade of responsive cells in adult-suppressed animals.

Animals↗
PubMed1974

Regulation of immune response by autogenous antibody against receptor.

BALB/c mice repeatedly immunized with Pneumococcus R36A vaccine produce antibodies to phosphorylcholine having the TEPC-15 myeloma idiotype (murine IgA myeloma protein that binds phosphorylcholine). The plaque-forming cell response to phosphorylcholine shows a decrease with repeated immunizations. In contrast, spleen cells from multiply immunized mice responded better in vitro than spleen cells from nonimmunized mice. The serum of animals immunized four or five times agglutinates TEPC-15-coated sheep erythrocytes. Inhibition of hemagglutination shows that the agglutinating activity is directed against the TEPC-15 idiotype. Sera from these mice, when added to cultures of normal spleen cells, specifically suppress the response to phosphorylcholine. The suppressive activity in the serum can be removed by solid absorption with TEPC-15. Evidently, repeated immunization with antigen induces two kinds of antibody responses: one directed against antigen and the other directed against the antibody to the antigen. It is proposed that this "auto" antibody against receptor is involved in the regulation of the immune response.

Adsorption↗
PubMed1974

[Pour-on method of administering chlormadinone acetate for estrus synchronization in heifers].

Experiments with chlormadinone (Bovisynchron) on 380 heifers and 60 cows showed that the drug was absorbed following pour-on application. A volume of 2 ml was rubbed into the skin of the back anterior to the sacrum, once daily for 15 days. Residual amounts were removed 24 hours after the final application by washing the hair coat with dimethyl sulphoxide solution. Insemination was performed at the same time as after oral administration. The pour-on method was particularly suitable for heifers and cows out at pasture.

Acetates↗