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Biomedical subjects

H KURTZ

Publications and source records attributed to H KURTZ.

7 recordsLinked to original sources
PubMed1955

Observations concerning a persisting infection of HeLa cells with poliomyelitis virus.

A culture of HeLa cells has been subjected to prolonged observation with the finding that periodically Type III poliomyelitis virus could be isolated from it. A requirement of the culture for survival was the presence in it of serum of certain individuals who had had previous experience with poliomyelitis virus. In the presence of serum containing no antibodies to poliomyelitis virus, the culture demonstrated spontaneous cytopathology. From certain series of passages virus could be isolated while attempts were unsuccessful from others also showing cellular disintegration. The conclusion is reached that the virus does not persist in the culture always in a state exhibiting the infectious property, rather what persists is the potentiality of the culture to give rise to fully active virus. The immune serum could inhibit the cytopathogenic effect of the virus without eliminating the infection.

Animals↗
PubMed1954

Growth characteristics of poliomyelitis virus in HeLa cell cultures; lack of parallelism in cellular injury and virus increase.

A detailed study of the cytological changes which are induced in HeLa cells by the Saukett strain of Type III poliomyelitis virus has been made. The observations were of cultures in which a single sequence of infection was induced. The cytological changes were examined in relation to the growth curve of the virus in the same type of culture. This curve showed a latent period of 4 to 5 hours, followed by a gradual release of virus over an interval of 6 to 7 hours. Changes in the staining character of the cells occurred before the major portion of the viral yield appeared. The infected cells exhibited a striking cytopathology with increased basophilia, nuclear pyknosis, and basophilic cytoplasmic granules. Individual cells showed characteristic differences in the rate at which the cytopathology progresses. The multiplication of the virus in HeLa cells was inhibited by fluorophenylalanine. The inhibitory effectiveness of the antimetabolite was related to the age of the infection. It apparently inhibits only an early stage of viral development. The inhibition is completely reversed by phenylalanine if the amino acid is added within 6 hours, not later, after the induction of virostasis. The data are interpreted in terms of the rate at which the ability of the infected cell to support viral synthesis was lost. Flurophenylalanine also inhibited the multiplication of HeLa cells; however, the effect upon the uninfected cell was reversible after 3 days, as indicated by viability after such treatment. While the fluoro derivative completely inhibited viral multiplication, it did not prevent the cytopathogenic effect of the virus. In the presence of fluorophenylalanine, the disintegration of an infected cell proceeded at what appeared to be the ordinary rate, without any increase of the infectious agent. Experimentally the processes leading to viral increase and to cellular injury have been shown to possess a significant degree of autonomy.

HeLa Cells↗
PubMed1952

The relation of herpes virus to host cell mitochondria.

The intracellular distribution of herpes virus in embryonic liver was investigated. 80 per cent of the virus was found in the cytoplasm in what appears to be a form uncombined with mitochondria or nuclei. A significant amount of the virus (16 per cent) was found bound to the mitochondria by an intimate attachment. Furthermore evidence was obtained that the mitochondria from liver tissue undergo a selective deterioration when infected with herpes virus. The concept is advanced that these organelles function in the development of virus.

Cell Nucleus↗