3H-trienbolone acetate (TBA) was injected/implanted in cattle and the distribution of radioactivity in liver and muscular tissue was determined, applying rigorously standardized organic or aqueous extraction procedures, either directly or following enzymatic hydrolysis and proteolytic procedures. These steps yielded almost 100% recovery of the radioactivity and indicated that only between 5% and 15% of the total residues present was extractable with organic solvents. The remaining radioactivity was either soluble in aqueous media or stayed bound to tissue structures. Similarly processed liver tissue from a calf treated with 3500 mg TBA 68 days prior to slaughter was examined by applying radioimmunoassay for the determination of TBA/trienbolone (TBOH). Indications of the presence of trienic-steroid type residues were only obtained for fractions containing residues extractable with organic solvents.

Anabolic Agents↗

PubMedDate not supplied

Application of Synovex-H in veal calves: steroid release and residues.

Four experiments were carried out with veal calves which were implanted subcutaneously with Synovex-H. Testosterone in blood plasma and also total oestrogens in plasma, urine and faeces during the trial and in liver, kidney and muscle after slaughter (62-75 days after implantation) were determined. Prepurification methods and radioimmunoassays for faeces and tissue samples were elaborated and validated. Variables between or within the trials were the sex, the dose (four pellets versus eight pellets; 25 mg testosterone propionate + 2.5 mg 17 beta-oestradiol-3-benzoate per pellet) and the implantation site (either on the base of the ear or on the middle of the pinna). Results from the blood plasma and the excreta showed that using four pellets implanted in the middle of the pinna guaranteed a continuous release in order that oestrogen levels were maximally doubled in comparison to controls. On the other hand, application of the same dose at the base of the ear was characterized by an accentuated burst during the first three days (10-fold elevation over controls). In plasma and excreta of male controls, higher oestrogen concentrations were measured as compared to the corresponding substrates of females. Comparing all groups, untreated males showed the highest testosterone levels. In all animals, residues of total oestrogens were of the same order of magnitude (kidney and liver 0.1-1.5 ng/g; muscle 0-32 pg/g) with somewhat higher means in the treated groups. Since all results are within the physiological scope, no risk for the consumer is visible after correct use of Synovex-H.

Anabolic Agents↗

PubMedDate not supplied

[Influence of the timing of an early postnatal booster vaccination on some fertility parameters in sows].

In a large pig production unit, with high prevalence of E. coli caused postpartal urogenital diseases of the breeding animals and diarrhoea of the piglets, the pregnant sows received during their late pregnancy a Porcovac plus E. coli vaccination. The vaccinated sows were assigned to two experimental groups and were treated postpartal (p. p.) as follows: Group 1 (421 sows): received an E. Coli booster on the first p. p. day Group 2 (299 sows): received a booster on the 7th p. p. day The following parameters were evaluated: A: Weaning-estrus-intervals B: Farrowing Rate The results showed a significant (p < 0.05) shorter weaning-estrus-interval (Parameter A) in group 1 when compared to group 2 (7.21 +/- 1.04 vs. 9.84 +/- 1.11). Regarding parameter B (farrowing rate) the groups showed no significant difference (group 1: 89.9% vs. group 2: 91.8%). It is the opinion of the authors that in large pig production units with high incidence of urogenital diseases an early p. p. E. coli Booster should be performed, because of its positive effect on the weaning-estrus-intervals.

Animals↗