Biomedical subjects

H Karlic

Publications and source records attributed to H Karlic.

22 records · Page 2Linked to original sources

PubMed1987

[Effect of ozone and ionizing radiation on an in vitro model--a pilot study of 4 gynecologic tumors].

In order to investigate the sensibility of cultured tumor cells to ozone and irradiation primary tissue cultures from one undifferentiated non-classified ovarian carcinoma, two solid adenocarcinomas of the ovary and one endometrial carcinoma were established. Cultivation was performed according to standard techniques involving the stem cell assay in soft agar as well as the monolayer technique in liquid nutrient media. The type of culture system did not influence the sensitivity of the culture cells to ozone and/or irradiation. Ozone treatment was performed with three different Ozone concentrations (0.03 ppm, 0.1 ppm, 0.3 ppm). Irradiation was done with 100 rd Ra226, Ir192 or Co60. A control experiment showed that the proliferative tendency of benign cells (skin fibroblasts) was not inhibited by the three ozone concentrations used in this study. Ra226 and even the combination of ozone and Radium did not influence the proliferative activity of these benign cells. Ir192 and Co60 were cytotoxic to benign as well as to carcinoma cells. Cultivated cells from endometrial carcinoma resisted to ozone treatment as well as to Ra226 (but they were destroyed by Ir119 and Co60). After pretreatment with ozone (0.1 ppm), Ra226 treatment of endometrial carcinoma cells induced a cytostatic effect implying that no cell divisions were observed after irradiation and the cells lysed within two weeks after irradiation. For the three ovarian carcinoma cell lines analysed in this study ozone treatment had a cytostatic effect even at the lowest concentration (0.03 ppm), with the two higher ozone concentrations a cytotoxic effect could be induced in ovarian carcinoma cells. Exclusive treatment with Ra226 induced a cytostatic effect, but it was cytotoxic after combination with ozone treatment of the lowest concentration (Ir192 and Co60 were cytotoxic in all cases). Our investigation confirmed the radiosensitizing effect of ozone treatment. Furthermore it was shown that exclusive ozone treatment even without combined irradiation displayed a selective cytotoxic action at the ovarian carcinoma cells.

Cells, Cultured↗

PubMed1986

[Simplified hamster ovum-sperm penetration test (HSPT) in routine sterility testing].

In 67 patients, in most of whom the spermiogram did not, a priori, furnish definitive proof of fertility, the hamster egg human sperm penetration test was performed as part of a screening programme for infertility therapy conducted from February 1984 to February 1985. Evaluation of the test was facilitated by identification of the decondensed spermatozoon head in the penetrated hamster egg with a simple fluorescein staining instead of the usual aceto-lacmoid staining. The penetration rate (= percentage proportion of the hamster ova liberated from the zona pellucida which are penetrated within 3 hours following incubation with 1 million motile spermatozoa) was evaluated statistically in comparison to the fertility criteria of the spermiogram (motility, density, pathoforms), in order to verify the existence of possible fertility disturbances due to inability to penetrate despite the motility of the spermatozoa. In 42 patients the diagnosis of infertility was confirmed by a penetration rate of less than 10%. In 17 patients fertility was conformed according to HSPT (penetration rate of over 20%), while in 7 patients fertility was in the lower normal range. In the light of the authors' findings fertility confirmed by the HSPT is a precondition of referral for extracorporeal insemination. In the light of the authors' findings intrauterine insemination appears advisable for women whose fertility is in the lower normal range according to HSPT.

Adult↗

PubMed1983

[Molecular-biological studies of the cervical secretion with one-dimensional electrophoresis].

In the present study the polypeptide composition of cervical mucus was analysed during the human menstrual cycle. Changes in the polypeptide profile as displayed by a very sensitive silverstaining method were consistent with cycle dependent variations in observed rheological properties of the mucus and activities (concentrations) of luteinising hormone (LH) (from comparatively analysed blood). It could be shown that whereas three main polypeptide components of the mucus (50 kd [kilodalton], 14 kd, 12 kd) remained constant the amount and intensity of other polypeptides increased towards midcycle (ovulation) and diminished on the days after ovulation. A 25 kd polypeptide (being a main component on the days before ovulation) disappeared at midcycle (on the day of ovulation). On the same day a new main peptide (8 kd) was observed, which was decreasing (in favour of the 25 kd peptide) on the days after ovulation. It is postulated that the described shift in the polypeptide pattern of the cervical mucus may be a result of the degradation of the light chain of IgG at midcycle.

Cervix Mucus↗

PubMedDate not supplied

Monitoring of hematopoietic recovery after autologous stem cell transplantation by analysis of G alpha 16 mRNA and CD34 surface glycoprotein.

The hematopoiesis-specific G protein alpha subunit G alpha16 was shown to be expressed in early normal and malignant hematopoietic cell lines and has been suggested to play an important role in signal transduction of hematopoiesis. We previously demonstrated a strict correlation of G alpha16 mRNA and CD34 antigen expression in peripheral blood stem cells (PBSC). In PBSC mobilization, both markers are detectable at the time of hematopoietic recovery and progenitor cell release. In this study the possible use of G alpha16 determination in peripheral blood samples for monitoring patients undergoing stem cell transplantation was investigated. Normal peripheral blood is negative for G alpha16 expression. In all five patients G alpha16 mRNA expression appeared shortly before the time of blood cell recovery. When tested together with CD34 (three cases) a pattern different from CD34 antigen expression was found, reflecting a different mechanism of action. In two cases with different time points of leukocyte and platelet recovery G alpha16 mRNA was detected at both time points but not in the interval, thus suggesting a role of G alpha16 in multipotent precursor cells. CD34 mRNA tested in three patients was not detected at any time; this argues for different regulation of CD34 and G alpha16 mRNA. G alpha16 may be used as an indicator of hematopoietic recovery after autologous stem cell transplantation, suggesting that there are cell type-specific G protein-mediated signal transduction pathways of early hematopoiesis.

Adult↗