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Biomedical subjects

H Katsuta

Publications and source records attributed to H Katsuta.

At least 37 records · Page 2Linked to original sources
PubMed1978

Pedigree analysis of the hypothermal recovery of the effects of gamma-rays on hepatoma cells.

Cultured hepatoma cells of the rat were irradiated with 400 to 900 R of gamma-rays. They were kept at room temperature for several hours before transferred to normal culture condition at 37 degrees C. The pedigree analysis of growing cells recorded by cinematography indicated a variety of lethal and non-lethal recovery events such as a decrease in lethal branch formation and an increase in locomotion. The so-called repair immediately after irradiation has many facets depending upon dose and duration of the low temperature treatment.

Animals↗
PubMed1977

Establishment of epithelial cell lines from rat glandular stomachs.

Nine epithelial cell lines were isolated from glandular stomach cells of fetal and suckling JAR-2 rats after the elimination of mesenchymal cells by mechanical and colonial isolation methods. Those cells have been proliferating continuously in a typical pavement-like arrangement. In early cultures a part of focal monolayers of small epithelial cells died simultaneously ("contact death"). The cells of three lines (RGS-2,RGS-5 and RGS-8) formed hemicysts when incubated for several weeks without subcultivation. Addition of But2cAMP and theophilline into culture medium enhanced the hemicyst formation by RGS-8 cells. Some evidence suggested that those three lines were originated from mucous epithelium of rat glandular stomach. These epithelial cell lines would be useful in the study of chemical carcinogenesis in culture.

Animals↗
PubMed1976

Kinetics of microcolonies of cultured mammalian cells after bleomycin treatment.

Cultured ascites hepatoma cells of the rat were treated with 400 to 900 R of gamma rays or 25 to 100 mug/ml of bleomycin for 30 min. The cells were followed up for 133 hr and the pedigrees were analyzed. Gamma rays and 2 low doses of bleomycin gave rise to typical reproductive death in generations 1 and 2 but 100 mug of the drug produced frequent interphase death. Natural death and induced death did not follow statistical randomness suggesting an existence of weak and sensitive clones in a population.

Bleomycin↗
PubMed1976

Carcinogenesis in tissue culture 27: heterotransplantation as an alternative in vivo assay for neoplastic state of cells in culture.

Cells of serially cultured strains and newly-isolated cells originating from rat liver and lung were heterotransplanted into pouches of golden hamsters treated with antithymocyte serum (ATS). The "take" was determined by histopathological examination of nodules formed by 3 weeks after the transplantation. The results obtained under this criteria showed that 9 of 11 cell strains backtransplantable to rats and 2 of 4 strains untransplantable were in parallel with those in rats. Three kinds of uncultured liver cells resulted in "no take".

Animals↗
PubMed1976

Carcinogenesis in tissue culture 26: malignant transformation of rat liver cells in vitro associated with paradoxical electrokinetic changes.

Periodical examinations of the electrophoretic mobility of rat liver cells of two culture strains, RLC-10(2) and JTC-25.P3, were performed after the treatment with 3.3 X 10(-6) M 4-nitroquinoline 1-oxide (4NQO) for 30 min in culture. As early as 47th day after a single treatment with 4NQO, both control and treated cells of RLC-10(2) were found to produce tumors in animals on back-transplantation. At this stage, however, little difference was yet observed in electrokinetic patterns between the treated cells and the original antecedent cells. In contrast, a subline, JTC-25.P3, treated twice with 4NQO under the same conditions demonstrated a malignant electrokinetic pattern resembling that of rat ascites hepatocarcinoma strains, although its tumorigenicity was not verified. These discrepancies between changes of electrokinetic pattern and tumorigenicity were discussed with special references to the antigenic difference between cultured cell strains and each original host rats.

Antibodies, Neoplasm↗
PubMed1975

Isolation and characterization of alkaline phosphatase-constitutive variants from Chinese hamster ovary cells (CHO-k1).

Pure clones with high alkaline phosphatase (ALP) activity were isolated from Chinese hamster ovary (CHO-K1) cells which showed no detectable ALP activity. ALP-positive cells appeared at the frequency of 10(-4) in the N-methyl-N'-nitrosoguanidine-treated cell population. With respect for cellular morphology, plating efficiency and several other enzymatic activities, no distinct difference was found between the original CHO-K1 cells and its ALP-positive variants, although some alterations in karyotype were found. Levels of ALP activity in these clones was stably maintained during serial cultivation. Some of the enzymological properties of ALP in the isolated clones were similar to those in bone or kidney.

Alkaline Phosphatase↗
PubMed1975

Kinetics of microcolonies of cultured mammalian cells after gamma irradiation.

The time of cell destruction was determined by pedigree analysis before macrocolony formation immediately after irradiation of gamma rays (400-900 R) in cultured rat hepatoma cells. Cell destruction occurred and was distributed to various generations in proportion to dose. The loss of reproductive integrity resulting in an extinction of a clone was limited in earlier generations, 0 to 2, but the loss of reproductive capacity was observed in later generations. It seems probable that two types of loss of reproduction come from random transmission of lethal signals produced in parent cells irrespective of the pattern of pedigrees.

Animals↗
PubMed1975

Interaction in culture between mouse ascites mammary carcinoma (MM2) cells and lymphoid cells of isologous mice.

MM2 cells, ascitic tumor originated from spontaneous mammary carcinoma of C3H/He mouse, were mix-cultured with lymphoid cells of thymus or mesenteric lymph nodes from isologous animals of the same sex. The interaction in culture between these cells was examined by time-lapse cinemicrography. In single culture, thymocytes, mesenteric lymph node lymphocytes and MM2 cells were kept for 7 days with little change in cell population. Lymphocytes of both sources showed a marked decrease in cell number when cultured together with MM2 cells, being evidently phagocytosed by MM2 cells. Lymphocytes from MM2-bearing mice or mice sensitized with deoxycholate-extracted MM2 antigen were also all phagocytosed. MM2 cells exhibited no sign of damage or degeneration due to the phagocytosis. Thymocytes were not phagocytosed by histiocytes obtained from ascitic fluid 3 days after i.p. injection of 5% starch suspension. Phagocytosis of erythrocytes or lymphoid cells from spleen by MM2 cells was not detected.

Animals↗
PubMed1975

A simple method for the cultivation of rat liver cells.

A simple method to culture rat liver parenchymal cells is described. Minced liver tissues were dissociated with no pre-perfusion by a newly developed enzyme, bacterial neutral proteinase, and cultured in the medium consisting of 10% fetal calf serum and 90% of a new synthetic medium, DM-153. By the adequate technique of primary dissociation and first subcultivation, parenchymal cells were selected. Cultivation in arginine-free medium was also useful in selecting them. The cultured cells exhibited activities of certain enzymes similar to those of liver cells in vivo. Cell strains have been successively established from liver tissues of embryo, suckling and adult rats. Liver cells, however, cultured in this way, can also be used for experiments in the early stage of serial cultivation.

Animals↗
PubMed1975

Action of bacterial neutral protease on the dispersion of mammalian cells in tissue culture.

The effect of a bacterial neutral protease on cultured cells was examined with special reference to its activity on the detachment of cells from glass surface, dissociation into single cells and to the inhibitory effect on cell proliferation. Fibroblasts of strains L-929 and CHO-K1, and epithelial cells of strains HeLa-S3, JTC-16 and RLC-10(2) were employed. Fibroblasts were detached and dissociated when this protease was added to MEM supplemented with fetal bovine serum. The epithelial cells were detached but were scarcely dissociated. Kinetic data were obtained concerning the dispersing characteristics of this bacterial neutral protease together with a few aspects on the difference between epithelial cells and fibroblasts in the nature of cell to cell, and cell to substrate interactions.

Bacillus↗
PubMed1975

An improved synthetic medium suitable for tissue culture of various mammalian cells.

A new mixture of synthetic medium, DM-153, was designed to obtain a higher rate of proliferation of various cells and to be adoptable for both of closed and opened systems of culture. The activity was compared to that of other synthetic media by cultivation for 2 weeks. In the presence of 10% (v/v) fetal calf serum, DM-153 exhibited the highest rate of proliferation of rat liver cells, strain RLC-10(2), than did Eagle MEM, DM-145 or lactalbumin hydrolysate which had been employed as a routine growth medium for the cells. In the absence of any high molecular weight substances in the medium, both of DM-153 and DM-145 showed higher rate of proliferation of rat liver cells, strain JTC-25-P3, than that obtained by MEM. In the case of primary culture of human blood lymphoid cells supplemented with 10% fetal calf serum, DM-153 resulted in apparently higher rate of cell survival than did RPMI-1640. The mixture DM-153 was confirmed to be suitable as a multi-purpose synthetic medium.

Animals↗
PubMed1975

Proliferation of mouse ascites mammary carcinoma MM2 cells in the primary culture.

Culture conditions suitable for active proliferation in primary culture of transplantable mouse ascites mammary carcinoma MM2 cells were investigated; kind of serum, its optimal concentration, composition of medium, inoculum size of cells, addition of ascites, conditioned medium or hormones, and others. The most active growth was obtained in the medium consisting of 5% fetal calf serum and 95% RPMI-1640, so far as examined. The addition of 1 mug/ml of estriol more accelerated the proliferation. MM2 cells were found to have preserved their tumorigenicity after 5 months of cultivation.

Animals↗
PubMed1975

Interaction in culture between mouse ascites hepatoma (MH-134) cells and lymphoid cells of isologous mice.

Thymocytes or lymphocytes of mesenteric lymph nodes were obtained from mice bearing subcutaneously mouse ascites hepatoma MH-134 for 5 to 40 days. These lymphoid cells were added into the cultures of MH-134 cells. Morphological changes of cells in the mixed cultures were observed by time-lapse cinemicrography for the period of 4 weeks. Lymphoid cells were phagocytosed by MH-134 cells, and, in most cases, the tumor cells did not undergo any damage due to the phagocytosis. The exceptional cases were as follows: When MH-134 cells were mixed with thymocytes from mice bearing MH-134 tumor for 5 days, MH-134 cells phagocytosed thymocytes but some of them died later. In the mixed cultures of MH-134 cells and thymocytes or mesenteric lymph node lymphocytes from mice bearing MH-134 for 14 or 15 days, MH-134 cells phagocytosed lymphoid cells but died later by the burst of cytoplasm. By the burst many lymphoid cells phagocytosed appeared from the broken cytoplasm of MH-134 cells and, in some cases, the lymphoid cells looked to be alive. These findings suggest the possibility that lymphoid cells attack tumor cells not only from the cell surface but also from the inside being phagocytosed by tumor cells.

Animals↗
PubMed1975

Carcinogenesis in tissue culture 24: tumorigenicity and aggregate-forming capacity of mammalian cells in culture.

Twenty two kinds of strains and substrains of mammalian cells were cultivated on a gyratory shaker to obtain cell aggregates. The relationship between the morphology of aggregates and tumorigenicity of the cells was investigated. In some groups of cells, the size of aggregates corresponded with their tumorigenicity, e.g. among rat liver cells untreated, hepatoma cells produced by back-transplantation of rat liver cells after the treatment with a chemical carcinogen in tissue culture, and the hepatoma cells passaged serially through rats. The correspondence, however, was generalized to not all the cell strains. It was impossible to identify the tumorigenicity of cells by their aggregate-forming capacity.

4-Nitroquinoline-1-oxide↗
PubMed1975

Effects of polyamines on the proliferation of mammalian cells in tissue culture.

Spermine, spermidine, putrescine and agmatine were examined for their cytotoxicity to mammalian cells in tissue culture. Spermine exhibited the highest cytotoxicity among them. It was followed by spermidine. Putrescine and agmatine showed little toxicity but rather acceleration of cell proliferation in some concentrations. Among 16 kinds of rat cells and strains and a mouse cell strain examined, liver cells, fibroblasts, and cells serially grown in a protein-free synthetic medium were sensitive to spermine in the order described. Normal liver cells were more sensitive than hepatoma cells, and normal fibroblasts were also more sensitive than sarcoma cells. Bovine serum albumin fraction markedly accelerated the cytotoxicity of spermine.

Agmatine↗