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Biomedical subjects

H Kaya

Publications and source records attributed to H Kaya.

At least 91 records · Page 5Linked to original sources
PubMed1988

[Blood reflux into cartridge in dental anesthesia. Detection of total protein and human hemoglobin in residual anesthetic solution].

The present study was designed to elucidate the blood reflux into cartridge under infiltration anesthesia. When imitation examination was carried out using pontamine blue dye solution in 7 kinds of syringes for the use of cartridge, dye reflux was observed in all of them. Compared 4 kinds of cartridges on the market, dye reflux was observed in all except one. The amounts of protein in residual anesthetic solution of cartridges after infiltration anesthesia were quantitatively measured by dye binding method. Human hemoglobin was also measured by enzyme immunoassay (EIA). The detection rates of protein and human hemoglobin were 26.2% (85/324) and 24.2% (24/99), respectively. From these facts that blood reflux cannot be avoided in the cartridge system, it is necessary to prohibit the reuse of residual anesthetic solution of cartridge as quickly as possible to avert the risk of cross infection of HB and AIDS virus.

Acquired Immunodeficiency Syndrome↗
PubMed1986

Differential inhibitory properties of dog and human alpha 1-proteinase inhibitors.

Dog alpha 1-proteinase inhibitor (alpha 1-PI) was found to be an effective inhibitor of bovine chymotrypsin and also of porcine pancreatic elastase as in the case of human inhibitor. The dog inhibitor inactivated both proteinases at a molar ratio of 1:1. However, compared to the human inhibitor, dog alpha 1-PI was a relatively poor inhibitor of bovine trypsin. The association rate constants (kass) of the interactions of dog alpha 1-PI with bovine chymotrypsin and with porcine elastase were determined to be 6.9 +/- 0.3 X 10(6) M-1 s-1 and 6.4 +/- 0.1 X 10(5) M-1 s-1, respectively. These values are 1.3- and 2.7-fold higher than the corresponding values for the human inhibitor. On the other hand, kass for the dog inhibitor with bovine trypsin (2.6 +/- 0.3 X 10(4)M-1 s-1) was found to be about 5 times smaller than that of the human inhibitor.

Amino Acid Sequence↗
PubMed1985

Effect of progesterone and its 17 alpha-hydroxyl derivative on human erythrocyte membrane.

Effect of progesterone and 17 alpha-hydroxyprogesterone on human erythrocyte membrane was investigated by three methods. Prompt hemolysis test: Progesterone progressively reduced the membrane fragility at the concentration of 1.25 X 10(-5)-2.0 X 10(-4) M, but 17 alpha-hydroxyprogesterone had almost no effect. Continuous MCV analysis: When erythrocytes were transferred into 0.9 (w/v) % NaCl solution without progesterone after incubation with 0.5 M d-glucose for 2 hr at 37 degrees C, erythrocytes markedly expanded up to about 140 microns 3 and subsequently returned to nearly the original volume (about 95 microns 3) within 30-40 sec. Progesterone reduced the reversibility of expanded erythrocytes at the concentration of 1.25 X 10(-5)-1.0 X 10(-4) M. 17 alpha-Hydroxyprogesterone had little effect on the reversibility. Scanning electron microscopy: After incubation in isotonic buffered saline solution without progesterone for 12 hr at 37 degrees C, most of the erythrocytes were transformed from discocytes to echinocytes and sphero-echinocytes (echinocytes+sphero-echinocytes, greater than 80%). Progesterone (5.0 X 10(-5) and 1.0 X 10(-4) M) inhibited this transformation, so that a greater number of erythrocytes remained as discocytes than in the control. Moreover, at higher concentration of progesterone, stomatocytes emerged prominently (1.0 X 10(-4) M progesterone), followed by the increase in spherocytes (2.0 X 10(-4) M). 17 alpha-Hydroxyprogesterone, however, merely prevented the echinocytic progress from I to II or III at the concentration of 5.0 X 10(-5)-2.0 X 10(-4) M and slightly increased the number of discocytes at the higher concentration (2.0 X 10(-4) M).(ABSTRACT TRUNCATED AT 250 WORDS)

17-alpha-Hydroxyprogesterone↗