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H Knüttel

Publications and source records attributed to H Knüttel.

6 recordsLinked to original sources

Host-plant-derived variation in ultraviolet wing patterns influences mate selection by male butterflies.

We report on the first case in which sequestered secondary plant compounds determine an insect's external appearance in the ultraviolet spectrum and thereby influence visually mediated mate choice. Larvae of the common blue butterfly Polyommatus icarus specifically sequester flavonoids in different amounts and types, depending on the part or species of food plant. During late pupal development the majority of ultraviolet-absorbing flavonoids are deposited in the wing scales. The flavonoid content of the larval diet thereby determines ultraviolet wing patterns. In laboratory and field experiments, male butterflies clearly preferred flavonoid-rich, ultraviolet-absorbing female dummies. This preference is mediated visually by the ultraviolet pattern of the wings. Food-plant parts and species vary in value as a food source, so ultraviolet wing patterns may signal mate quality and are not a species-specific characteristic. We discuss the use of principal component analysis in analysing spectral data in the context of visual communication. We propose the alternative application of confidence intervals of averaged spectra as a novel straightforward statistical method for comparing groups of spectra in a manner that is independent of assumptions about the visual system of the receiver. In addition, they can be used to give confidence intervals to derived measures of colour such as quantum catch by photoreceptors.

Animals↗

Flavonoid wing pigments increase attractiveness of female common blue (Polyommatus icarus) butterflies to mate-searching males.

Common blue butterflies (Polyommatus icarus) sequester flavonoids from their larval host plants and allocate these UV-absorbing pigments to the wings. In field experiments using dummies constructed from female butterflies, mate-searching males inspected flavonoid-rich dummies more intensively than those with little or no flavonoids. Flavonoid content as signalled by UV-wing pattern may indicate ontogenetically determined female quality or enhance detectability to males.

Animals↗

Comparison of the membrane-bound hydrogenases from Alcaligenes eutrophus H16 and Alcaligenes eutrophus type strain.

Whereas the membrane-bound hydrogenase from Alcaligenes eutrophus H16 is an integral membrane protein and can only be solubilized by detergent treatment, the membrane-bound hydrogenase of Alcaligenes eutrophus type strain was found to be present in a soluble form after cell disruption. For the enzyme of A. eutrophus H16 a new, highly effective purification procedure was developed including phase separation with Triton X-114 and triazine dye chromatography on Procion Blue H-ERD-Sepharose. The purification led to an homogeneous hydrogenase preparation with a specific activity of 269 U/mg protein (methylene blue reduction) and a yield of 45%. During purification and storage the enzyme was optimally stabilized by the presence of 0.2 mM MnCl2. The hydrogenase of A. eutrophus type strain was purified from the soluble extract by a similar procedure, however, with less specific activity and activity yield. Comparison of the two purified enzymes revealed no significant differences: They have the same molecular weight, both consist of two different subunits (Mr = 62,000, 31,000) and both have an isoelectric point near pH 7.0. They have the same electron acceptor specificity reacting with similar high rates and similar Km values. The acceptors reduced include viologen dyes, flavins, quinones, cytochrome c, methylene blue, 2,6-dichlorophenolindophenol, phenazine methosulfate and ferricyanide. Ubiquinones and NAD were not reduced. The two hydrogenases were shown to be immunologically identical and both have identical electrophoretic mobility. For the membrane-bound hydrogenase of A. eutrophus H16 it was demonstrated that this type of hydrogenase in its solubilized, purified state is able to catalyze also the reverse reaction, the H2 evolution from reduced methyl viologen.

Alcaligenes↗

[Dialysis treatment of advanced diabetic nephropathy (author's transl)].

Dialysis treatment of patients with diabetic nephropathy turns out to be difficult because of numerous late complications which arise in addition to the renal disease and which often influence the direction of the course of the disease. But this experience does not in the least justify the exclusion generally of patients with diabetic nephropathy from dialysis the-rapy. Hemodialysis and peritoneal dialysis are equally suitable for the treatment of renal insufficiency; patients with accumulated hemorrhagic complications (e. g. vitreous hemorrhages) should be treated by peritoneal dialysis for preference, and those with a predominant hypertension by hemodialysis. Early preparation for dialysis treatment is of great importance.

Blood Glucose↗