Macromolecular interactions: tracing the roots.
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Enzymes↗
Biomedical subjects
H Knull
Publications and source records attributed to H Knull.
Structure within eukaryotic cytoplasm and its relationship to glycolytic metabolism.
Taken together, the results reviewed here indicate that both structural proteins and enzymes exist in a relatively mobile, uncomplexed form and in a relatively immobile form, complexed with the matrix. The relative amounts of free and complexed forms of each protein are dependent upon the local concentrations of both small molecules and other macromolecules and hence may vary in time and space throughout the cell. Free and cytomatrix-bound enzymes exchange rapidly, while free and cytomatrix-bound structural proteins exchange more slowly. These two distinct time scales suggest that the slowly exchanging structural proteins form the core of fibrous structural elements--having many stabilizing intermolecular contacts with near neighbours--whereas the more rapidly exchanging enzymes adsorb to the surface of the structural elements and have fewer near neighbour contacts. The hierarchical nature of these associations is depicted schematically in Figure 3. Metabolism is proposed to proceed primarily via transport of small metabolites rather than by transport of enzymes, which may be organized in functional clusters to facilitate, metabolic regulation.
Cell Count↗
Aldolase-tubulin interactions: removal of tubulin C-terminals impairs interactions.
Aldolase copelleted with taxol stabilized microtubules with a Bmax = 0.74 moles of aldolase per mole of tubulin dimer. Removal of the carboxy terminals from microtubules with limited subtilisin digestion, decreased binding to 0.16 moles of aldolase per mole of tubulin dimer. Aldolase inhibited subtilisin cleavage of the C-terminals while triose phosphate isomerase, an enzyme that does not interact with microtubules, did not affect subtilisin activity. These data indicate that the carboxy terminals are involved in tubulin-aldolase interactions.
Animals↗
A review of the biochemical pathways studied and abnormalities reported in the Rett syndrome.
A review of the current research on selected biochemical pathways and reported abnormalities in the Rett syndrome (RS) is presented. While RS has a clinical course that is similar to known metabolic diseases, consistent abnormal biochemical findings in RS patients have not been identified.
Biogenic Amines↗
"Montana gin": ingestion of commercial products containing denatured alcohol among native Americans.
A preliminary investigation of the ingestion of commercial products containing alcohol among Native Americans in the United States is described. A survey of Indian Health Service Clinic physicians found 1121 persons to be abusing these products. The products most frequently ingested were hair spray and spray disinfectants. The abuse of these products, nicknamed "Montana Gin," has been found in 14 states. Very little is known about the effects of ingestion of these products and further research is needed.
Alcoholism↗