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H Komiya

Publications and source records attributed to H Komiya.

At least 55 records · Page 3Linked to original sources
PubMed1981

Nucleotide sequence of 5S ribosomal RNA from the posterior silk glands of Bombyx mori.

A low molecular weight RNA fraction was obtained from the posterior silk glands of Bombyx mori. 5S rRNA was purified from this fraction by gel filtration on a column of Sephadex G-100 and electrophoresis on a discontinuous polyacrylamide gel devised for large scale preparation. The nucleotide sequence of this RNA was determined mainly by a chemical sequencing method on polyacrylamide gels using RNA labeled with [32P]pCp at the 3' terminus (1). An enzymatic sequencing method on gels using 32P-labeled RNA at the 5' end (2) and conventional sequence analyses of the complete digests of the unlabeled RNA with RNase T1 [EC 3.1.27.3] and RNase A [EC 3.1.27.5] were used for complete determination of the total sequence. 16 base substitutions were observed between B. mori and Drosophila melanogaster 5S rRNAs.

Animals↗
PubMed1981

The nucleotide sequence of 5S ribosomal RNA from Schizosaccharomyces pombe.

The nucleotide sequence of 5S rRNA from the fission yeast, S. pombe, has been established by post labeling procedures combined with cataloging RNase T1- and A-oligonucleotides derived from unlabeled 5S rRNA. The sequence consists of 119 nucleotides without a modified base and shows more dissimilarities (at 38 positions) from that of S. cerevisiae than from that of humans (at 33 positions).

Ascomycota↗
PubMed1981

The nucleotide sequence of 5S ribosomal RNA from slime mold Physarum polycephalum.

The nucleotide sequence of 5S ribosomal RNA from plasmodia of the slime mold Physarum polycephalum was determined as pppGGAUGCGGC CAUACUAAGG 20 AGAAAGCACC 30 UCAUCCCGUC 40 CGAUCUGAGA 50 AGUUAAGCUC 60 CUUCAGGCGU 70 GGUUAGUACU 80 GGGGUGGGGG 90 ACCACCUGGG 100 AAUCCCACGU 110 GCUGCAUUCU 120 Uoh by chemical and enzymatic gel sequencing technics using 3' and 5' end-labeled RNA. This RNA is very different from 5S rRNA of the cellular slime mold Dictyostelium discoideum (36 nucleotides are different), and shows greater similarity to 5S rRNAs from Protozoa and Metazoa than to those from fungi.

Base Sequence↗
PubMed1980

Nucleotide sequence of 5S ribosomal RNA from Lingula anatina. A study on the molecular evolution of 5S ribosomal RNA from a living fossil.

By chromatography on columns of DEAE-Sephadex A-50 and Sephadex G-100, and electrophoresis on polyacrylamide gel, 5S rRNA was purified from a low-molecular-weight RNA fraction extracted from the total tissues of Lingula anatina. Complete digests of the 5S rRNA with RNase T1 [EC 3.1.4.8] and pancreatic RNase [EC 3.1.4.22] were sequenced by conventional column chromatography procedures. The nucleotide sequence of this RNA was determined mainly by a chemical method for sequencing the RNA 3' end-labeled with 32P (1), with the complement of the oligonucleotide catalog obtained by the complete RNase digestions of the RNA. By comparing the sequences of several invertebrate, vertebrate, and Chlorella 5S rRNAs, a phylogenic tree of the rRNAs was constructed and the time of divergence of Lingula was estimated.

Animals↗
PubMed1980

Nucleotide sequences and evolutional aspect of 5S ribosomal RNAs from Lingula and silkworm.

Ribosomal 5S RNAs were highly purified from the total tissues of Lingula anatina and the posterior silk glands of Bombyx mori. The nucleotide sequences of the RNAs were determined by a chemical method for rapidly sequencing on gels of 3' end-labeled RNA, with the aid of conventional sequence analysis of the complete RNase digests of the unlabeled RNAs. By comparing the sequences with those of several animal and Chlorella 5S rRNAs, a phylogenic tree of these 5S rRNAs was constructed.

Animals↗
PubMed1979

A case of 21q--syndrome with normal SOD-1 activity.

A female infant with del(21)(pter leads to q22.1--2:) constitution identified by G, Q, and R banding is reported. She had marked mental and physical retardation, generalized hypertonia, microphthalmia with persistent hypoplastic primary vitreous, blepharochalasia, high nasal bridge, micrognathia, malformed ears with preauricular pits, and overlying fingers. The assay for superoxide dismutase-1 (SOD-1) activity in the patient revealed a normal value and it was suggested that the gene locus for SOD-1 in 21q22.2 leads to qter is not compatible with the presence of the gene dosage effect in the monosomic state.

Abnormalities, Multiple↗
PubMed1979

Nucleotide sequence of 5S ribosomal RNA from rainbow trout (Salmo gairdnerii) liver.

Staring from low molecular weight RNA obtained from rainbow trout (Salmo gairdnerii) liver, 5S ribosomal RNA (rRNA) was highly purified by successive chromatography on columns of DEAE-Sephadex A50 and Sephadex G100. Products of complete and partial digestions on this RNA with pancreatic ribonuclease (RNase A) [EC 3.1.4.22] and RNase T [EC 3.1.4.8] were isolated and sequenced by conventional and high-performance liquid chromatography (HPLC) procedures. The nucleotide sequence of this RNA thus established was compared with those of five other vertebrae 5S rRNAs, and the rates of base substitution per site per year were found to be nearly constant in these RNAs. The analyses of the partial digests of the trout 5S rRNA revealed several sites susceptible to RNase attack, which could be accounted for by the secondary structure model for eukaryotic 5S rRNAs proposed by Nishikawa and Takemura (1).

Animals↗
PubMed1979

Methods for sequencing oligoribonucleotides and RNA by high performance liquid chromatography.

Conditions were established for the separation and quantitative determination of ribonucleosides, mono- and oligo-ribonucleotides by high-performance liquid chromatography (HPLC) on columns of AS-Pellionex SAX and AL-Pellionex WAX. By combining a high-speed UV spectrum monitor with an HPLC apparatus, products of RNase digestions of oligonucleotides and 5S ribosomal RNA (rRNA) were identified by measuring their UV spectra under continuous solvent flow, and also from their retention times on the columns (positions of elution). It took only 10 to 30 min for one chromatography run and required less than 0.01 A260 unit of sample per nucleotide material in each peak.

Animals↗