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H Kramer

Publications and source records attributed to H Kramer.

At least 19 recordsLinked to original sources
PubMed1992

Subtilisin removes the surface layer of the phage fd coat.

The major coat protein of native filamentous phage fd is vulnerable to digestion by subtilisin, but not by any of a number of other proteolytic enzymes. Degradation by the non-specific protease subtilisin occurs at specific sites in the N-terminal portion of g8p. The N-terminal part of the protein is considered to be the outer layer of a two-layered coat. Thus, subtilisin treatment results in a monolayered phage particle. These particles possess the morphology and stability of native phage fd. Furthermore, subtilisin proteolysis proved to be an efficient instrument in detecting variations in the topology of the g8p of related filamentous phages.

Amino Acid Sequence↗
PubMed1979

[Immunologic diagnosis].

The development of the immunologic basic research and a deeper insight into the pathogenesis of diseases with changed immune reactivity led to an intensivation of the clinic-immunologic in-vivo- and in-vitro-diagnostics. At the instance of the pulmonary diseases according to the classification of Gell and Coombs the immunologic-diagnostic possibilities and problems are shown. Here is referred to the importance of the use of standardized tests and of the wide replaceability of in-vivo-techniques by in-vitro-methods.

Anti-Glomerular Basement Membrane Disease↗
PubMed1979

[Evaluation of lymphocyte transformation test: relationship between nucleus and cell sizes of PHA-stimulated lymphocyes (author's transl)].

A methodological comparision of evaluation of lymphocyte transformation test is described. Three experts examined smear-slides of cells from five lymphocyte cultures and the corresponding controls in a blind study. In the morphological differentiation of 100 cells per slide in comparison to 1 000 cells per slide it was found a good intraindividually but a unsatisfactory interindividually correlation. 1 000 cells from stimulated and non-stimulated cultures shows the greatest difference for the parameter "total cell size" and the lowest difference for the "nucleus plasma relation" by the planimetrically imeasurement.

Cell Nucleus↗
PubMed1979

[Investigations for the use of micromodification of lymphocyte transformation test in patients with lung disease (author's transl)].

In a preliminary study a micromodification of lymphocyte transformation test was examined for use in patients with specific and nonspecific lung diseases. Investigation was carried out about the influence of different conditions in the cell culture system. A micromodification was defined and introduced in the clinic simultaneously to the conventional macromodification of lymphocyte transformation test. This micro lymphocyte transformation test is of use for determination of cell mediated immunity. However, in the described form it is doubtful for the detection of present specific sensibilization.

Dose-Response Relationship, Drug↗
PubMed1978

[Use of delayed cutaneous hypersensitivity skin test and in vitro assays of lymphocyte function to tumour associated antigen in patients with lung cancer (author's transl)].

Twenty one patients with lung cancer and twelve patients with unspecified lung disease were evaluated by means of delayed cutaneous hypersensitivity skin tests with tumour associated antigen. Nine of these patients with lung cancer and seven healthy persons were evaluated by in vitro assays of lymphocyte function to tumour associated antigen (TAA) from "Cancerotest Dessau". We did not find a statistically significant lymphocyte transformation to the testing concentration of TAA. For the same patients with lung cancer and controls we found statistically significant results of the mean lymphocyte transformation to phytohemagglutinin. It has been shown that the delayed cutaneous hypersensitivity skin tests demonstrated "false positive" reactions to TAA. It is possible that this is an expression of the irritative effects of TAA. It is estimated that the application of the antigens in this form presented and in this chosen examination conditions, does not allow for definite diagnostic assessment to be expected.

Antigens, Neoplasm↗