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H Kresbach

Publications and source records attributed to H Kresbach.

At least 19 recordsLinked to original sources
PubMed1990

Quantitative evaluation of melanoma cell invasion in three-dimensional confrontation cultures in vitro using automated image analysis.

Tumor invasion is a crucial feature of tumor growth in vivo. Confrontation cultures of multicellular melanoma spheroids and embryonic chick heart fragments provide a model for invasive growth in vitro. We have developed an image analysis method, which facilitates the objective measurement of tumor cell invasion in this model. Cryostat sections of confrontation cultures were immunohistochemically stained with an antiserum directed against the stromal component for automated recognition of the stroma tissue. The slides were automatically processed by a grey level based computerized image analysis system. On Spearman's rank correlation test, 25 out of 39 parameters correlated with the reference value of invasion, which was derived from the subjective evaluation of five independent observers. Two parameters combining the stroma margin and the total amount of stroma tissue completely reproduced the judgement of the morphologists in our test set. The quantitative evaluation of tumor invasion in vitro by automated image analysis may be helpful in pharmacologic and pathogenetic studies of tumor growth.

Animals↗
PubMed1990

Histophotometry of protein thiols and disulphides in tissue samples from the human uterine cervix and the skin reveals a "field effect" as well as an "extended field effect" of malignant tumours.

Fresh frozen and fixed serial sections were stained with 2,2'-dihydroxy-6,6'-dinaphthyldisulfide (DDD) and Fast blue B for reactive protein thiols (PSHr) and total reactive protein sulfur (TRPS). The mean optical densities of PSHr and TRPS determined histophotometrically at a distinct part of a tissue were related to each other (PSHr: TRPS). If this quotient has been determined, e.g. for normal epithelium and the adjacent stroma, both quotients can be related to each other by a double quotient (Q PSHr: TRPS). With the aid of the double quotient highly significant differences could be found between tumours and normal tissue from patients without tumour of the human uterine cervix. Similar differences exist between normal skin from healthy patients and skin tumours. Q PSHr: TRPS revealed similar differences to exist between normal tissue of patients without tumour and apparently normal tissue in the neighbourhood of tumours of the uterine cervix and of skin ("field effect" of tumours). Histophotometric investigations on abdominal skin (and skin of breast) showed highly significant differences between normal skin of patients without tumour and patients with various kinds of tumours of the uterine cervix, ovaries, liver and breast ("extended field effect" of tumours).

Cervix Uteri↗
PubMed1990

[Acute febrile neutrophilic dermatosis (Sweet syndrome). A retrospective clinical and histological analysis].

Acute febrile neutrophilic dermatosis (Sweet's syndrome) was first described in 1964. The condition presents with rapidly developing inflammatory plaques, a neutrophilic dermal infiltrate, fever and leucocytosis. In this study, clinical and histological features of 18 patients with Sweet's syndrome have been analysed. The arms (83%), face (67%) and legs (67%) were the most common sites of involvement. Morphologically, plaques, nodes and pseudo-vesicles prevailed. Elevated ESR (89%), fever (72%) and peripheral blood leucocytosis (44%) were noted in many, but not all, cases. Histology revealed a dermal infiltrate with numerous neutrophils (100%) without vasculitis. Nuclear dust (72%) and extravasation of erythrocytes (44%) were also encountered. Collagen degeneration was not observed. Differential diagnosis included erythema multiforme, erythema nodosum and adverse drug reaction. Possible causative factors observed were upper respiratory tract infection, gastrointestinal infection, and malignancies. Sweet's syndrome may be considered as an extreme manifestation within a continuous spectrum of cutaneous reactions to various stimuli or as an ill-defined entity that has some overlap with other dermatoses.

Acute Disease↗
PubMed1989

[Polymyalgia rheumatica with drug eruption--an important differential diagnosis to dermatomyositis].

With regard to certain clinical features, polymyalgia rheumatica (PR) may closely resemble dermatomyositis. In contrast to dermatomyositis, PR usually does not show any cutaneous manifestations, although there might be seen concomitant giant cell arteritis. Furthermore, we do not find muscle enzymes in the serum with PR, and there is no histologic evidence of myositis. In rare cases, however, PR may be associated with cutaneous drug eruption and/or non-specific increase of muscle enzymes, which might cause considerable difficulties regarding the diagnostic differentiation from dermatomyositis.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMed1989

Histophotometric quantification of the field effect and the extended field effect of tumors.

Histophotometric investigations have been made on samples of human skin. Fresh frozen serial sections were fixed and stained for either reactive protein thiols (PSHr) or total reactive protein sulphur (TRPS) using modifications of the DDD-Fast blue B-method. In addition, total protein thiols (PSHt) were stained with the Mercurochromcyanide-method, and proteins were stained using a modified amido-black procedure. Significant differences were found between the different tumours investigated and normal tissue, and also between apparently normal tissue adjacent to the tumours and normal tissue from patients without tumour. To reveal such tumour-related changes of apparently normal tissue, termed the field effect of tumours, a double quotient had to be calculated from the PSHr-and TRPS-values determined from both epithelium (epidermis) and connective tissue. In addition, abdominal skin was investigated from patients without tumour and patients with tumours of the female genital tract, liver or breast. With the aid of the double quotient procedure, highly significant differences were found between normal abdominal skin of patients without tumours versus similar samples taken from patients with tumours. The tumour-related changes found with abdominal skin distant from the tumours have been termed the extended field effect of tumours. These general tumour-related changes, independent of the size, state or degree of malignancy of the distant tumour, could be shown to be due to changes in abdominal dermis.

Female↗
PubMed1988

[Direct light microscopy of pigment tumors of the skin].

Clinical guidelines for the diagnosis of pigmented skin lesions are not always reliable. Incident light microscopy provides an interesting approach to solving this problem. For this investigation a stereomicroscope, a glass slide and immersion oil are used. The various in vivo criteria of this method, which go beyond the changes discernible by the naked eye, are correlated with the histopathological structures. Incident light microscopy opens up a new dimension of clinical morphology for the diagnosis and differential diagnosis of malignant melanoma, dysplastic nevi or non-melanocytic pigmented neoplasms and facilitates a more precise preoperative assessment of these lesions.

Diagnosis, Differential↗
PubMed1988

[Kaposiform angiodermatitis (pseudo-Kaposi's disease) on an amputation stump. A new entity].

Kaposiform cutaneous lesions were observed on the amputation stump of a 65-year-old male whose leg had been amputated above the knee. Histological examination revealed pronounced stasis dermatitis. This finding, together with phlebectasias and fine arborized cutaneous varices on the stump end, suggested that the pathogenesis may involve venous congestion. This may be caused by suction in the prosthesis socket and compression of the stump at the socket edge.

Aged↗
PubMed1987

[Remarks on contact eczemas--etiopathogenetic considerations based on immunohistochemical studies].

Functionally defined inflammatory cells can be selectively labeled in tissue sections by immunohistochemical methods. We investigated the cellular infiltrate in acute toxic contact dermatitis and various forms of contact eczema and other inflammatory dermatoses. In all cases, the so-called "superficial T-cell pattern" was evident, which comprised T-lymphocytes, predominantly helper/inducer cells, as well as Langerhans cells/indeterminate cells. The numerical density of these cell types, which represent the cellular prerequisites for delayed hypersensitivity reactions, turned out to be markedly increased in affected skin. Compared with normal skin, therefore, lesional skin provides a different immunological microenvironment. We conclude that a negative patch test reaction in normal skin does not exclude the possibility that the same allergen may provoke and enhance immunological reactions in eczematous skin. Eczema is regarded as an abnormal reaction of the organism towards exogenous factors; the abnormal reaction may be confined to sites with a considerable infiltrate. This might explain the pronounced chronicity of contact eczemas, even after withdrawal of the presumed cause, and the existence of chronic allergic contact eczemas without positive patch test results. The inflammatory infiltrate may be interpreted as a major predisposing factor for further immunological reactions at the same site.

Antibodies, Monoclonal↗
PubMed1987

[Acroangiodermatitis (pseudo-Kaposi disease)].

We present clinical, angiological, and histopathological findings concerning different kinds of so-called "kaposiform" acroangiodermatitis. In particular, we refer to the etiopathogenesis, prognosis, differential diagnosis, and therapy of each entity. The similarities between the so-called "kaposiform" acroangiodermatitis and Kaposi's sarcoma are primarily derived from clinical criteria. In contrast to Kaposi's sarcoma, the histopathological picture of acroangiodermatitis usually shows stasis dermatitis.

Acrodermatitis↗
PubMed1986

[Balanitis and differential diagnosis].

In this synopsis the most common balanitides are presented and illustrated in a plate. Special consideration is given the differentiation of the balanitides from each other as well as from malignant diseases. Some modern concepts in the etiopathogenetic development and treatment are mentioned.

Bacterial Infections↗
PubMed1986

Histophotometric quantification of protein and reactive proteinthiols in invasive carcinomas of the skin.

Frozen sections cut from 14 samples of invasive carcinomas of the skin were stained with Amido black for protein determination and with dihydroxydinaphthyldisulphide fast blue to quantify reactive protein thiols (PSHr) and were then analysed microphotometrically. It was found that all of the samples exhibited significant reductions in protein levels (49%-74%) and PSHr levels (32%-53%) as compared to normal epidermis. Thus, the content of proteins of PSHr groups was 1.7 times greater in the malignant tissue examined than in normal epidermis. These results are in accordance with those previously obtained in basal-cell epitheliomas.

Amido Black↗
PubMed1986

[Retroviruses and the immune system].

Three examples are presented to illustrate the importance of retroviruses and associated oncogenes in neoplasms of the immune system. In adult T-cell leukemia/lymphoma the HTLV I-provirus is often integrated in the human genome on chromosome 8 adjacent to the c-myc protooncogene. Cytogenetic studies of Burkitt's lymphoma (translocation of a chromosome 8 fragment) and a case of mycosis fungoides (trisomy of chromosome 8) indicate that the same oncogene may possibly be activated not only by retroviruses, but also by chromosomal aberrations.

Acquired Immunodeficiency Syndrome↗
PubMed1985

Significant decreases in the intensity of staining for proteins and protein thiols in basal-cell epitheliomas (basaliomas) as compared to normal skin.

Microphotometric measurements of fast reacting protein thiols (PSHr) and proteins were performed on freshly frozen sections of samples from normal skin (26 cases as controls) and from 45 basal cell epitheliomas (basalioma; BCE). The intensity of the staining (E/micron2) for both proteins and PSHr was significantly higher in normal epidermis than in the adjacent dermis. The values of QE (quotient of values observed in the epidermis divided by those observed in the dermis) were calculated to be 3.48 for proteins (QE, Prot) and 4.62 for PSHr (QE, PSHr). In cases of BCE, significantly lower QE values were found: QE, Prot = 2.16 and QE, PSHr = 1.72. The decrease of QE, PSHr was due to a decrease in the staining intensity observed in the BCEs, whereas practically no changes occurred in the adjacent dermis. The decrease of QE, Prot was mainly caused by a decrease in the staining intensity in the BCE (by 68%) as well as in the adjacent dermis (by 36%). By dividing the mean extinction value (E/micron2) for PSHr by the E/micron2 for proteins, a new quotient, PSHr/Prot, is obtained which can serve as a quantitative measure of the content of the tissue proteins of PSHr. The proteins of normal epidermis contained more PSHr than dermal proteins. The proteins of BCEs also contained more PSHr than those of the adjacent dermis, but the PSHr/Prot values of both tissues were 1.5 to 1.6 times greater than the corresponding values for normal epidermis and dermis, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Basal Cell Carcinoma↗
PubMed1985

Significance of non-lymphoid ('accessory') cells in malignant lymphomas and pseudolymphomas of the skin.

We investigated the co-distribution of lymphocyte subpopulations and non-lymphoid 'accessory' cells in 35 cases of cutaneous lymphoproliferative diseases (T-cell lymphoma, 10 cases; B-cell lymphoma, 17 cases; pseudolymphoma, 8 cases) using immunohistochemical methods. T-zone histiocytes and particularly Langerhans cells were abundant in all cutaneous T-cell lymphomas, but were also found in B-cell lymphomas. T-zone histiocytes were associated with T-lymphocytes, especially T-helper cells, but not with T-suppressor cells. Dendritic reticulum cells were essentially confined to well differentiated germinal centres. Macrophages occurred in both lymphomas and pseudolymphomas without definite relationship with either B- or T-cells. In malignant lymphomas of high grade malignancy, macrophages represented the only non-lymphoid cell type. Our results indicate that malignant lymphoid cells, like normal lymphocytes, require definite micro-environments which are, at least in part, maintained by certain non-lymphoid cells.

Antigen-Presenting Cells↗
PubMed1984

[A tricho-odonto-onychial subtype of ectodermal dysplasia].

We report on a case of " uncombable hair syndrome" combined with additional hair changes ( peripilar casts), periodic shedding of nails, abnormality of teeth, dysplasia of the nipples , Morbus Scheuermann, and atopic disorders with specific IgE sensibilisation . Pili trianguli et canaliculi as well as peripilar casts were electron-microscopically examined. The presented case might be interpreted as a special tricho-odonto- onychial subtype of ectodermal dysplasia.

Adolescent↗
PubMed1984

Germinal center cell-derived lymphomas of the skin.

Germinal center cell-derived (GCC) malignant lymphomas are B-cell tumors that represent neoplastic equivalents of normal lymphoid follicles. These lymphomas include, in the Kiel classification, three main types: centrocytic lymphoma, centroblastic-centrocytic lymphoma, and centroblastic lymphoma. We have diagnosed 13 cases of germinal center cell-derived cutaneous lymphomas among 178 evaluated malignant lymphomas of the skin. Some clinical, histologic, and immunologic aspects are discussed in this article.

Aged↗
PubMed1981

[Applications of the peroxidase-anti-peroxidase technique in dermatology (author's transl)].

The immunohistological findings are reported in 62 cases of malignant lymphomas, pseudolymphomas and malignant histiocytic disorders of the skin. Paraffin-embedded tissue was analyzed with the Peroxidase-Antiperoxidase (PAP) Technique for the presence of intracytoplasmic immunoglobulin (IgM, IgA, IgG, Kappa, Lambda) ana lysozyme. It can be stated that the PAP technique appears to be a valuable aid in interpreting and differentiating selected material of cutaneous lymphoproliferative and histiocytic disorders. The method supplements routine histological and histochemical staining procedures.

Histiocytoma, Benign Fibrous↗