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Biomedical subjects

H Krumme

Publications and source records attributed to H Krumme.

7 recordsLinked to original sources
PubMed1985

Nuclear polymorphism in osteosarcomas as a prognostic factor for the effect of chemotherapy. A quantitative study.

A current strategy for osteosarcoma treatment is neo-adjuvant chemotherapy prior to the resection of the tumour. It appears that some tumours respond very well to the cytostatic therapy, while others show little or no effect. It is desirable to be able predict the response of the tumour before starting chemotherapy. 16 biopsy specimens from patients with osteosarcoma who had been treated according to the protocol of the study COSS-80 and COSS-82 were examined. 100 tumour cells from each biopsy have been measured by an electronic interactive image analysis system (IBAS II; Kontron/ZEISS). After completion of chemotherapy en bloc resection of the tumour was performed. The entire surgical specimen was completely examined at two levels by means of undecalcified sections, and assigned a grade for the effect of chemotherapy analogous to the grading of Salzer-Kuntschik et al. (1983). The quantitative analysis of tumour cell nuclei revealed two different patterns of nuclear sizes, which were correlated significantly with the chemotherapy response (P less than 0.002). Tumour cell nuclei of well responders were significantly larger and showed a greater variance in size (mean value 66 + 41 micron 2), than those of poor responders (mean value 38 + 18 micron 2). We conclude from our results that quantitative analysis and classification of nuclear size of osteosarcoma cells may be useful for predicting chemotherapy response in patients with osteosarcoma.

Adolescent↗
PubMed1984

Computerized simulation of bone remodeling: graphic demonstration of dynamic processes.

A computerized simulation model has been developed to study the histologic patterns of dynamic remodeling processes in trabecular bone. The osteoid seam thickness and the distance between the tetracycline labels are calculated based on (partly hypothesized) parameter values, such as matrix appositional rate, mineralization rate, number and life span of the osteoclasts, resorption rate, and mineralization lag time. The results of a simulation run are demonstrated using computer graphics. Such simulation results cast doubts on the validity of some approaches that attempt to infer details of remodeling processes from histologic measurements. For example, the distance between the tetracycline labels obtained by simulation illustrates the difficulty of over-or underestimating the matrix appositional rate by measuring it from a histologic section.

Bone Resorption↗
PubMed1983

Morphological changes in osteosarcoma after chemotherapy--COSS 80.

Forty-five osteosarcomas were investigated by special methods such as preparation of undecalcified bone tumor tissue, imprint cytology, histochemistry, and quantitative analysis. The morphological regression grades and their relation to chemotherapy are reported by Salzer-Kuntschik et al. (1983). The results presented demonstrate that smaller osteosarcomas respond more favorably than larger tumors. The function of bone-tumor cells, e.g., osteoid production, trabecular tumor bone formation, and mineralization, seems to be more important for the sensitivity to chemotherapy than the cell polymorphism. The estimation of bone-tumor morphology in at least two total areas of the bone tumor is essential in borderline cases (grade III/grade IV). Imprint cytology is very helpful for rapid estimation of the effect of therapy and the demonstration of cellular polymorphism. At the moment, we are not able to determine the effect of chemotherapy from the first biopsy before starting chemotherapy.

Alkaline Phosphatase↗
PubMed1981

[A special case of familial colonic polyposis].

An unusual case of familial polyposis coli has been reported in which polypoid lesions of the stomach and the ileum were found in association to the adenomatosis coli. The histological examination showed that these lesions were not adenomatous polyps, as supposed by the radiographs, but non-inflammatory cysts located within the intact fundal glands of the stomach, and lymphoid polyps ("lymphcysts") of the ileum. The genetic cause is possibly hamartoma. Both lesions are benign and there is no indication for an operation. The familial adenomatosis coli itself has a well-known special tendency to become malignant. The only way to interrupt the tendency is to remove the colon by colectomy.

Adenocarcinoma↗
PubMed1980

[Transhepatic endless drainage according to Goetze-Dick in stenoses of the intrahepatic and extrahepatic bile ducts (author's transl)].

Results and experiences in 51 transhepatic endless drainage operations according to the method of Goetze-Dick are dealt with, the indication as well as the technique of the operation are discussed in detail. The advantage of this method is based on the fact, that the drain can be easily changed. So patients with inoperable obstructive jaundice can often be given many month of worthwhile life. The extracorporal part of the drain is hardly found to trouble the patients. Also benign intrahepatic obstructions, where no bile duct is left to be anastomized, can be operated on satisfactorily with this method. In spite of the blunt penetration of the liver necessitated by this technique, intra- and postoperative complications are rare. Postoperative care is paramount in avoiding cholangitis.

Adult↗